The neuroprotective effect of exogen melatonin upon fetal hippocampus damage caused by high-dose caffeine administration in pregnant rats

IF 1.7 4区医学 Q3 DEVELOPMENTAL BIOLOGY

International Journal of Developmental Neuroscience Pub Date : 2024-03-12 DOI:10.1002/jdn.10323

Yağmur Köse, Cansın Şirin, Ali Çağlar Turgut, Canberk Tomruk, Yiğit Uyanıkgil, Mehmet Turgut

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Abstract

Objective

The aim of this study is to evaluate whether exogenous melatonin (MEL) mitigates the deleterious effects of high-dose caffeine (CAF) administration in pregnant rats upon the fetal hippocampus.

Materials and methods

A total of 32 adult Wistar albino female rats were divided into four groups after conception (n = 8). At 9–20 days of pregnancy, intraperitoneal (i.p.) MEL was administered at a dose of 10 mg/kg/day in the MEL group, while i.p. CAF was administered at a dose of 60 mg/kg/day in the CAF group. In the CAF plus MEL group, i.p. CAF and MEL were administered at a dose of 60 and 10 mg/kg/day, respectively, at the same period. Following extraction of the brains of the fetuses sacrificed on the 21st day of pregnancy, their hippocampal regions were analyzed by hematoxylin and eosin and Cresyl Echt Violet, anti-GFAP, and antisynaptophysin staining methods.

Results

While there was a decrease in fetal and brain weights in the CAF group, it was found that the CAF plus MEL group had a closer weight average to that of the control group. Histologically, it was observed that the pyramidal cell layer consisted of 8–10 layers of cells due to the delay in migration in hippocampal neurons in the CAF group, while the MEL group showed similar characteristics with the control group. It was found that these findings decreased in the CAF plus MEL group.

Conclusion

It is concluded that high-dose CAF administration causes a delay in neurogenesis of the fetal hippocampus, and exogenous MEL is able to mitigate its deleterious effects.

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外源性褪黑素对孕鼠高剂量咖啡因导致的胎儿海马损伤的神经保护作用

研究目的本研究旨在评估外源性褪黑素（MEL）是否能减轻大剂量咖啡因（CAF）对妊娠大鼠胎儿海马的有害影响：将32只成年Wistar白化雌性大鼠受孕后分为四组（n = 8）。怀孕 9-20 天时，MEL 组腹腔注射 MEL，剂量为 10 毫克/千克/天；CAF 组腹腔注射 CAF，剂量为 60 毫克/千克/天。在CAF加MEL组中，同期给药的i.p. CAF和MEL剂量分别为60毫克/千克/天和10毫克/千克/天。在妊娠第21天时剖腹取出胎儿大脑，用苏木精、伊红、甲酚紫、抗GFAP和抗嗜酸性粒细胞蛋白染色法对其海马区进行分析：虽然CAF组的胎儿和大脑体重有所下降，但发现CAF加MEL组的平均体重与对照组更接近。组织学观察发现，由于 CAF 组海马神经元迁移延迟，锥体细胞层由 8-10 层细胞组成，而 MEL 组与对照组表现出相似的特征。研究发现，这些结果在 CAF 加 MEL 组中有所减少：结论：大剂量 CAF 会导致胎儿海马神经元发生延迟，而外源性 MEL 能够减轻其有害影响。

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来源期刊

International Journal of Developmental Neuroscience 医学-发育生物学

CiteScore

3.30

自引率

5.60%

发文量

审稿时长

6-12 weeks

期刊介绍： International Journal of Developmental Neuroscience publishes original research articles and critical review papers on all fundamental and clinical aspects of nervous system development, renewal and regeneration, as well as on the effects of genetic and environmental perturbations of brain development and homeostasis leading to neurodevelopmental disorders and neurological conditions. Studies describing the involvement of stem cells in nervous system maintenance and disease (including brain tumours), stem cell-based approaches for the investigation of neurodegenerative diseases, roles of neuroinflammation in development and disease, and neuroevolution are also encouraged. Investigations using molecular, cellular, physiological, genetic and epigenetic approaches in model systems ranging from simple invertebrates to human iPSC-based 2D and 3D models are encouraged, as are studies using experimental models that provide behavioural or evolutionary insights. The journal also publishes Special Issues dealing with topics at the cutting edge of research edited by Guest Editors appointed by the Editor in Chief. A major aim of the journal is to facilitate the transfer of fundamental studies of nervous system development, maintenance, and disease to clinical applications. The journal thus intends to disseminate valuable information for both biologists and physicians. International Journal of Developmental Neuroscience is owned and supported by The International Society for Developmental Neuroscience (ISDN), an organization of scientists interested in advancing developmental neuroscience research in the broadest sense.