{"title":"Genome-wide identification of bHLH transcription factors and expression analysis under drought stress in Pseudoroegneria libanotica at germination","authors":"Xingguan Zhai, Xia Wang, Xunzhe Yang, Qingxiang Huang, Dandan Wu, Yi Wang, Houyang Kang, Lina Sha, Xing Fan, Yonghong Zhou, Haiqin Zhang","doi":"10.1007/s12298-024-01433-w","DOIUrl":null,"url":null,"abstract":"<p>The basic helix-loop-helix (bHLH) transcription factor family is the second largest in plants. bHLH transcription factor is not only universally involved in plant growth and metabolism, including photomorphogenesis, light signal transduction, and secondary metabolism, but also plays an important role in plant response to stress. However, the function of bHLH TFs in <i>Pseudoroegneria</i> species has not been studied yet. <i>Pseudoroegneria</i> (Nevski) Á. Löve is a perennial genus of the <i>Triticeae</i>. <i>Pseudoroegneria</i> species are mostly distributed in arid/semi-arid areas and they show good drought tolerance. In this study, we identified 152 PlbHLH TFs in <i>Pseudoroegneria libanotica</i>, which could be classified into 15 groups. Collinearity analysis indicates that 122 <i>PlbHLH</i> genes share homology with <i>wbHLH</i> genes in wheat, and it has lower homology with <i>AtbHLH</i> genes in <i>Arabidopsis</i>. Based on transcriptome profiling under an experiment with three PEG concentrations (0%, 10%, and 20%), 10 up-regulated genes and 11 down-regulated <i>PlbHLH</i> genes were screened. Among them, <i>PlbHLH6</i>, <i>PlbHLH55</i> and <i>PlbHLH64</i> as candidate genes may be the key genes related to drought tolerance response at germination, and they have been demonstrated to respond to drought, salt, oxidative, heat, and heavy metal stress in yeast. This study lays the foundation for an in-depth study of the biological roles of <i>PlbHLHs</i> in <i>Pse. libanotica</i>, and discovered new drought-tolerance candidate genes to enhance the genetic background of <i>Triticeae</i> crops.</p>","PeriodicalId":20148,"journal":{"name":"Physiology and Molecular Biology of Plants","volume":"284 1","pages":""},"PeriodicalIF":3.4000,"publicationDate":"2024-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Physiology and Molecular Biology of Plants","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s12298-024-01433-w","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
The basic helix-loop-helix (bHLH) transcription factor family is the second largest in plants. bHLH transcription factor is not only universally involved in plant growth and metabolism, including photomorphogenesis, light signal transduction, and secondary metabolism, but also plays an important role in plant response to stress. However, the function of bHLH TFs in Pseudoroegneria species has not been studied yet. Pseudoroegneria (Nevski) Á. Löve is a perennial genus of the Triticeae. Pseudoroegneria species are mostly distributed in arid/semi-arid areas and they show good drought tolerance. In this study, we identified 152 PlbHLH TFs in Pseudoroegneria libanotica, which could be classified into 15 groups. Collinearity analysis indicates that 122 PlbHLH genes share homology with wbHLH genes in wheat, and it has lower homology with AtbHLH genes in Arabidopsis. Based on transcriptome profiling under an experiment with three PEG concentrations (0%, 10%, and 20%), 10 up-regulated genes and 11 down-regulated PlbHLH genes were screened. Among them, PlbHLH6, PlbHLH55 and PlbHLH64 as candidate genes may be the key genes related to drought tolerance response at germination, and they have been demonstrated to respond to drought, salt, oxidative, heat, and heavy metal stress in yeast. This study lays the foundation for an in-depth study of the biological roles of PlbHLHs in Pse. libanotica, and discovered new drought-tolerance candidate genes to enhance the genetic background of Triticeae crops.
期刊介绍:
Founded in 1995, Physiology and Molecular Biology of Plants (PMBP) is a peer reviewed monthly journal co-published by Springer Nature. It contains research and review articles, short communications, commentaries, book reviews etc., in all areas of functional plant biology including, but not limited to plant physiology, biochemistry, molecular genetics, molecular pathology, biophysics, cell and molecular biology, genetics, genomics and bioinformatics. Its integrated and interdisciplinary approach reflects the global growth trajectories in functional plant biology, attracting authors/editors/reviewers from over 98 countries.