Genome-wide identification of bHLH transcription factors and expression analysis under drought stress in Pseudoroegneria libanotica at germination

IF 3.4 3区 生物学 Q1 PLANT SCIENCES
Xingguan Zhai, Xia Wang, Xunzhe Yang, Qingxiang Huang, Dandan Wu, Yi Wang, Houyang Kang, Lina Sha, Xing Fan, Yonghong Zhou, Haiqin Zhang
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Abstract

The basic helix-loop-helix (bHLH) transcription factor family is the second largest in plants. bHLH transcription factor is not only universally involved in plant growth and metabolism, including photomorphogenesis, light signal transduction, and secondary metabolism, but also plays an important role in plant response to stress. However, the function of bHLH TFs in Pseudoroegneria species has not been studied yet. Pseudoroegneria (Nevski) Á. Löve is a perennial genus of the Triticeae. Pseudoroegneria species are mostly distributed in arid/semi-arid areas and they show good drought tolerance. In this study, we identified 152 PlbHLH TFs in Pseudoroegneria libanotica, which could be classified into 15 groups. Collinearity analysis indicates that 122 PlbHLH genes share homology with wbHLH genes in wheat, and it has lower homology with AtbHLH genes in Arabidopsis. Based on transcriptome profiling under an experiment with three PEG concentrations (0%, 10%, and 20%), 10 up-regulated genes and 11 down-regulated PlbHLH genes were screened. Among them, PlbHLH6, PlbHLH55 and PlbHLH64 as candidate genes may be the key genes related to drought tolerance response at germination, and they have been demonstrated to respond to drought, salt, oxidative, heat, and heavy metal stress in yeast. This study lays the foundation for an in-depth study of the biological roles of PlbHLHs in Pse. libanotica, and discovered new drought-tolerance candidate genes to enhance the genetic background of Triticeae crops.

Abstract Image

bHLH 转录因子的全基因组鉴定及干旱胁迫下假鹅掌楸萌芽期的表达分析
基本螺旋-环-螺旋(bHLH)转录因子家族是植物中第二大转录因子家族。bHLH转录因子不仅普遍参与植物的生长和代谢,包括光形态发生、光信号转导和次生代谢,而且在植物对胁迫的响应中也发挥着重要作用。然而,bHLH TFs 在假鹅掌楸物种中的功能尚未得到研究。Pseudoroegneria (Nevski) Á.Löve 是一种多年生三尖杉科属植物。Pseudoroegneria 物种主要分布在干旱/半干旱地区,具有良好的耐旱性。本研究在 Pseudoroegneria libanotica 中发现了 152 个 PlbHLH TFs,可分为 15 组。共线性分析表明,122个PlbHLH基因与小麦中的wbHLH基因具有同源性,而与拟南芥中的AtbHLH基因同源性较低。根据三种 PEG 浓度(0%、10% 和 20%)实验下的转录组分析,筛选出 10 个上调基因和 11 个下调 PlbHLH 基因。其中,PlbHLH6、PlbHLH55和PlbHLH64作为候选基因可能是萌芽期耐旱响应的关键基因,它们在酵母中对干旱、盐胁迫、氧化胁迫、热胁迫和重金属胁迫均有响应。该研究为深入研究PlbHLHs在Pse.libanotica中的生物学作用奠定了基础,并发现了新的耐旱候选基因,有助于增强三尖杉科作物的遗传背景。
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来源期刊
CiteScore
7.10
自引率
0.00%
发文量
126
期刊介绍: Founded in 1995, Physiology and Molecular Biology of Plants (PMBP) is a peer reviewed monthly journal co-published by Springer Nature. It contains research and review articles, short communications, commentaries, book reviews etc., in all areas of functional plant biology including, but not limited to plant physiology, biochemistry, molecular genetics, molecular pathology, biophysics, cell and molecular biology, genetics, genomics and bioinformatics. Its integrated and interdisciplinary approach reflects the global growth trajectories in functional plant biology, attracting authors/editors/reviewers from over 98 countries.
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