Targeted Metagenomics Identification of Microbiome in Preschools exposed to air Pollutants and Their Association with Respiratory Health symptom, Allergy and Eczema

IF 2.9 4区 环境科学与生态学 Q3 ENVIRONMENTAL SCIENCES
Cynthia Oluchi Onwusereaka, Juliana Jalaludin, Khairul Nizam Mohd Isa, Syafinaz Binti Amin Nordin, Suhaili Abubakar, Veronica Chua Poo Choo
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引用次数: 0

Abstract

Microorganisms are an important aspect of the environment which have significant effects on human health. Children are more vulnerable to the effects of microbial pollutants, pathogens and allergens. Microbial components of indoor air cannot be overemphasized due to its vastness and ubiquitous nature. This is a cross sectional comparative study which analysed microbiome in 10 preschools according to their level of exposure to outdoor air pollutant sources using amplicon sequencing technique. A total of 20 dust samples were collected from indoor and outdoor environment and analysed for the presence of bacteria and fungi. A detailed questionnaire was used to assess the respiratory health status of the respondents. Indoor air quality was assessed by measuring the level of particulate matter (PM2.5 and PM10), temperature, relative humidity, air velocity, and CO2. Exposed area recorded higher level of PM10 and CO2 (p <0.05) while temperature, relative humidity and air velocity were higher in the comparative area (p <0.05). Health symptoms were significantly higher in exposed area for cough, eczema and allergy symptoms (p <0.05). Predominant bacteria species includes Acinetobacter_radioresistens, Acinetobacter_seifertii, Lactobaccillus_iners, Pseudomonas_B_luteola, and Franconibacter_pulveris while predominant fungi species includes Rhodosporidioboluc_fluvialis, Rhodotorula_kratochvilovae, Aspergillus_penicilloides, Aspergillus_gracilis, Curvularia_sorghina, Eupenidiella_venezuelensis, Rhodosporidiobolus_ruineniae, Bipolaris_papendorfii and Curvularia_lunata. Cough symptom was strongly associated with PM10 (AOR =1.27), carpeting (AOR =1.62), Cladosporium dominicanum (AOR =2.12) and Aureobasidium gracilis (AOR =2.04). Eczema was strongly associated with keeping furry pet (AOR =5.46) and Rhodotorula_toruloides (1.02). Allergy was strongly associated with PM10 (AOR =3.23), Megasporoporia_bannaensis (AOR =1.43) and Malassezia_restricta (AOR =2.10). Preschools located close to outdoor air pollutants were more exposed to pollutants and microbiome from indoor and outdoor sources. Health symptoms were significantly associated with air pollutants, therefore proper measures should be implemented to mitigate the exposure to microbiome, infiltration of outdoor air pollutants and health effects on children.

Abstract Image

暴露于空气污染物的学龄前儿童微生物组的靶向元基因组学鉴定及其与呼吸道健康症状、过敏和湿疹的关系
微生物是环境的一个重要方面,对人类健康有重大影响。儿童更容易受到微生物污染物、病原体和过敏原的影响。室内空气中的微生物成分种类繁多,无处不在,因此怎么强调都不为过。这是一项横断面比较研究,利用扩增子测序技术,根据 10 所幼儿园暴露于室外空气污染源的程度,对其微生物组进行了分析。研究人员从室内和室外环境中共收集了 20 份灰尘样本,并对样本中的细菌和真菌进行了分析。采用详细的问卷调查来评估受访者的呼吸系统健康状况。室内空气质量通过测量颗粒物(PM2.5 和 PM10)、温度、相对湿度、风速和二氧化碳水平进行评估。暴露区的 PM10 和 CO2 水平较高(p <0.05),而对比区的温度、相对湿度和风速较高(p <0.05)。暴露区的咳嗽、湿疹和过敏症状等健康症状明显较高(p <0.05)。主要细菌种类包括放线杆菌(Acinetobacter_radioresistens)、塞弗提杆菌(Acinetobacter_seifertii)、乳酸杆菌(Lactobaccillus_iners)、假单胞菌(Pseudomonas_B_luteola)和弗兰科尼氏菌(Franconibacter_pulveris),主要真菌种类包括红孢子菌(Rhodosporidioboluc_fluvialis)、而主要的真菌种类包括Rhodotorula_kratochvilovae、Aspergillus_penicilloides、Aspergillus_gracilis、Curvularia_sorghina、Eupenidiella_venezuelensis、Rhodosporidiobolus_ruineniae、Bipolaris_papendorfii 和 Curvularia_lunata。咳嗽症状与 PM10(AOR =1.27)、地毯(AOR =1.62)、Cladosporium dominicanum(AOR =2.12)和 Aureobasidium gracilis(AOR =2.04)密切相关。湿疹与饲养毛茸茸的宠物(AOR =5.46)和Rhodotorula_toruloides(1.02)密切相关。过敏与 PM10(AOR =3.23)、Megasporoporia_bannaensis(AOR =1.43)和 Malassezia_restricta (AOR =2.10)密切相关。距离室外空气污染物较近的学龄前儿童更容易受到来自室内和室外的污染物和微生物群的影响。健康症状与空气污染物密切相关,因此应采取适当措施,减少微生物群的暴露、室外空气污染物的渗入以及对儿童健康的影响。
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来源期刊
Air Quality Atmosphere and Health
Air Quality Atmosphere and Health ENVIRONMENTAL SCIENCES-
CiteScore
8.80
自引率
2.00%
发文量
146
审稿时长
>12 weeks
期刊介绍: Air Quality, Atmosphere, and Health is a multidisciplinary journal which, by its very name, illustrates the broad range of work it publishes and which focuses on atmospheric consequences of human activities and their implications for human and ecological health. It offers research papers, critical literature reviews and commentaries, as well as special issues devoted to topical subjects or themes. International in scope, the journal presents papers that inform and stimulate a global readership, as the topic addressed are global in their import. Consequently, we do not encourage submission of papers involving local data that relate to local problems. Unless they demonstrate wide applicability, these are better submitted to national or regional journals. Air Quality, Atmosphere & Health addresses such topics as acid precipitation; airborne particulate matter; air quality monitoring and management; exposure assessment; risk assessment; indoor air quality; atmospheric chemistry; atmospheric modeling and prediction; air pollution climatology; climate change and air quality; air pollution measurement; atmospheric impact assessment; forest-fire emissions; atmospheric science; greenhouse gases; health and ecological effects; clean air technology; regional and global change and satellite measurements. This journal benefits a diverse audience of researchers, public health officials and policy makers addressing problems that call for solutions based in evidence from atmospheric and exposure assessment scientists, epidemiologists, and risk assessors. Publication in the journal affords the opportunity to reach beyond defined disciplinary niches to this broader readership.
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