The structure of inactivated mature tick-borne encephalitis virus at 3.0 Å resolution.

IF 8.4 2区医学 Q1 IMMUNOLOGY

Emerging Microbes & Infections Pub Date : 2024-12-01 Epub Date: 2024-03-11 DOI:10.1080/22221751.2024.2313849

Evgeny B Pichkur, Mikhail F Vorovitch, Alla L Ivanova, Elena V Protopopova, Valery B Loktev, Dmitry I Osolodkin, Aydar A Ishmukhametov, Valeriya R Samygina

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Abstract

Tick-borne encephalitis virus (TBEV) causes a severe disease, tick-borne encephalitis (TBE), that has a substantial epidemiological importance for Northern Eurasia. Between 10,000 and 15,000 TBE cases are registered annually despite the availability of effective formaldehyde-inactivated full-virion vaccines due to insufficient vaccination coverage, as well as sporadic cases of vaccine breakthrough. The development of improved vaccines would benefit from the atomic resolution structure of the antigen. Here we report the refined single-particle cryo-electron microscopy (cryo-EM) structure of the inactivated mature TBEV vaccine strain Sofjin-Chumakov (Far-Eastern subtype) at a resolution of 3.0 Å. The increase of the resolution with respect to the previously published structures of TBEV strains Hypr and Kuutsalo-14 (European subtype) was reached due to improvement of the virus sample quality achieved by the optimized preparation methods. All the surface epitopes of TBEV were structurally conserved in the inactivated virions. ELISA studies with monoclonal antibodies supported the hypothesis of TBEV protein shell cross-linking upon inactivation with formaldehyde.

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分辨率为 3.0 Å 的灭活成熟蜱传脑炎病毒结构。

蜱传脑炎病毒（TBEV）会引起一种严重的疾病--蜱传脑炎（TBE），这种疾病在欧亚大陆北部具有重要的流行病学意义。尽管有有效的甲醛灭活全病毒疫苗，但由于疫苗接种覆盖率不足，每年仍有 10,000 到 15,000 例蜱传脑炎病例登记在册，而且还有零星的疫苗突破病例。抗原的原子分辨率结构将有利于改良疫苗的开发。与之前公布的 TBEV 毒株 Hypr 和 Kuutsalo-14（欧洲亚型）的结构相比，分辨率的提高得益于通过优化制备方法提高了病毒样本的质量。在灭活的病毒中，TBEV 的所有表面表位在结构上都保持一致。用单克隆抗体进行的 ELISA 研究支持了 TBEV 蛋白外壳在甲醛灭活后发生交联的假设。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Emerging Microbes & Infections IMMUNOLOGY-MICROBIOLOGY

CiteScore

26.20

自引率

2.30%

发文量

276

审稿时长

20 weeks

期刊介绍： Emerging Microbes & Infections is a peer-reviewed, open-access journal dedicated to publishing research at the intersection of emerging immunology and microbiology viruses. The journal's mission is to share information on microbes and infections, particularly those gaining significance in both biological and clinical realms due to increased pathogenic frequency. Emerging Microbes & Infections is committed to bridging the scientific gap between developed and developing countries. This journal addresses topics of critical biological and clinical importance, including but not limited to: - Epidemic surveillance - Clinical manifestations - Diagnosis and management - Cellular and molecular pathogenesis - Innate and acquired immune responses between emerging microbes and their hosts - Drug discovery - Vaccine development research Emerging Microbes & Infections invites submissions of original research articles, review articles, letters, and commentaries, fostering a platform for the dissemination of impactful research in the field.