Association between MUC1 rs4072037 polymorphism and Helicobacter pylori in patients with gastric cancer.

Pub Date : 2024-01-01 DOI:10.22088/cjim.15.1.15

Ramin Shekarriz, Hadi Jabbari, Reza Alikhani, Reza Alizadeh-Navaei, Mohammad Bagher Hashemi-Soteh

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Abstract

Background: The MUC1 gene encodes glycoproteins attached to cell membrane that play a protective role in gastric cancer and protect epithelial surfaces against external factors such as Helicobacter pylori. H. pylori infection can induce a cascade of innate and acquired immune responses in gastric mucosa. Relationship between rs4072037G>A polymorphism of MUC1 gene and increased susceptibility to H. pylori infection aimed to investigate in patients with gastric cancer in Mazandaran, northern Iran.

Methods: A case-control study was conducted on 99 patients with gastric cancer (H. pylori positive and negative) and 98 controls (H. pylori positive and negative) without gastric cancer (confirmed by pathological biopsy samples obtained during endoscopy). H. pylori infection was diagnosed by histological examination using Giemsa staining. Genomic DNA extracted from peripheral blood was analyzed by PCR-RFLP technique.

Results: Analysis of all genetic models showed no significant relationship between rs4072037G>A polymorphism and risk of gastric cancer (GC). The relationship between H. pylori infection and rs4072037G>A polymorphism showed an increased susceptibility to gastric cancer in both positive and negative H. pylori groups (including case and control groups). The genetic model of GA/GG and H. pylori- positive versus GA/GG and H. pylori-negative showed a significantly increased susceptibility to gastric cancer (OR=0.251, CI: 0.128-0.493, P=0.000).

Conclusion: These findings indicate that rs4072037G>A polymorphism may interact with H. pylori infection to increase the risk of GC.

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胃癌患者的 MUC1 rs4072037 多态性与幽门螺旋杆菌之间的关系

背景：MUC1 基因编码附着在细胞膜上的糖蛋白，在胃癌中发挥保护作用，并保护上皮表面免受幽门螺杆菌等外界因素的侵害。幽门螺杆菌感染可诱导胃黏膜产生一系列先天性和获得性免疫反应。旨在研究伊朗北部马赞达兰地区胃癌患者中 MUC1 基因 rs4072037G>A 多态性与幽门螺杆菌感染易感性增加之间的关系：对 99 名胃癌患者（幽门螺杆菌阳性和阴性）和 98 名未患胃癌的对照组（幽门螺杆菌阳性和阴性）（通过内窥镜检查获得的病理活检样本确认）进行了病例对照研究。幽门螺杆菌感染是通过吉氏染色法进行组织学检查确诊的。从外周血中提取的基因组 DNA 采用 PCR-RFLP 技术进行分析：结果：对所有遗传模型的分析表明，rs4072037G>A 多态性与胃癌（GC）风险无显著关系。幽门螺杆菌感染与 rs4072037G>A 多态性之间的关系显示，幽门螺杆菌阳性组和阴性组（包括病例组和对照组）的胃癌易感性均增加。GA/GG和幽门螺杆菌阳性与GA/GG和幽门螺杆菌阴性的遗传模型显示，胃癌易感性显著增加（OR=0.251，CI：0.128-0.493，P=0.000）：这些研究结果表明，rs4072037G>A 多态性可能与幽门螺杆菌感染相互作用，从而增加患胃癌的风险。

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