{"title":"Treatment Promotion of Osteoporotic Fractures by microRNA-320 Nanocapsules Through Stimulating Bone Marrow Mesenchymal Stem Cells","authors":"Ligang Qian, Qinggui Li, Qiao Ren","doi":"10.1166/jbn.2024.3784","DOIUrl":null,"url":null,"abstract":"We aimed to explore the mechanism underlying microRNA-320 (miR-320)’s role in osteoporotic fractures. miR-320 nanoparticles were prepared and their characterization was detected by Zetasizer Nano and triethylamine (TEA). miR-320 nanoparticles were interacted with bone marrow mesenchymal\n stem cells (BMSCs). Then we conducted MTT to assess cytotoxicity in BMSCs and determined genes expression. A mouse fracture model was established and treated with miR-320 nanoparticles or pore nanoparticles. The release of miR-320 and the bone repair at the fracture site were detected. Treatment\n of Ceramic matrix composites (CMCS) (miR-320) sensitive to Matrix metalloproteinase (MMP) released miR-320 to bone defect, which promoted the transcription of osteogenic genes and stimulated the osteogenesis. Finally, treatment of miR-320 nanoparticles facilitated bone repair of mouse osteoporotic\n defect. MMP-sensitive nanocapsules loaded with miR-320 can promote osteogenic potential and stimulate fracture repair, providing insight into novel treatment against osteoporotic fracture.","PeriodicalId":15260,"journal":{"name":"Journal of biomedical nanotechnology","volume":null,"pages":null},"PeriodicalIF":2.9000,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of biomedical nanotechnology","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1166/jbn.2024.3784","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Medicine","Score":null,"Total":0}
引用次数: 0
Abstract
We aimed to explore the mechanism underlying microRNA-320 (miR-320)’s role in osteoporotic fractures. miR-320 nanoparticles were prepared and their characterization was detected by Zetasizer Nano and triethylamine (TEA). miR-320 nanoparticles were interacted with bone marrow mesenchymal
stem cells (BMSCs). Then we conducted MTT to assess cytotoxicity in BMSCs and determined genes expression. A mouse fracture model was established and treated with miR-320 nanoparticles or pore nanoparticles. The release of miR-320 and the bone repair at the fracture site were detected. Treatment
of Ceramic matrix composites (CMCS) (miR-320) sensitive to Matrix metalloproteinase (MMP) released miR-320 to bone defect, which promoted the transcription of osteogenic genes and stimulated the osteogenesis. Finally, treatment of miR-320 nanoparticles facilitated bone repair of mouse osteoporotic
defect. MMP-sensitive nanocapsules loaded with miR-320 can promote osteogenic potential and stimulate fracture repair, providing insight into novel treatment against osteoporotic fracture.