The Specificity of Interactions between Endoinulinase from Aspergillus ficuum and Mono-, Di-, and Polysaccharides

IF 4.033 Q4 Biochemistry, Genetics and Molecular Biology
S. M. Makin, A. N. Dubovitskaya, D. Yu. Bogomolov, M. S. Kondratyev, M. G. Holyavka, V. G. Artyukhov
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引用次数: 0

Abstract

The aim of this study was to analyze the features of the spatial organization of the endoinulinase molecule from Aspergillus ficuum after its binding to mono-, di-, and polysaccharides. This study examined changes in volume and number of internal cavities upon binding of inulinase to mono- (glucose, fructose), di- (sucrose, mannose), and polysaccharides (inulin). Transformations in the quantity and length of tunnels and pores were described, and the reorganization of the composition and localization of charged and hydrophobic amino acid residues clusters on the surface of the enzyme molecule was analyzed. It was shown that the models of inulinase in the complex with sucrose (an alternative substrate) and mannose (an activator) exhibit the same types of internal structures. A similar pattern was found in the formation of complexes with fructose (a reaction product) and glucose (an inhibitor). In addition, it was established that both charged and hydrophobic clusters do not undergo significant changes in chemical composition after the binding of inulinase to mono-, di-, and polysaccharides, i.e., the interaction between inulinase and carbohydrates mentioned above primarily affects the internal structures of the enzyme. The specificity of the binding of inulinases to various ligands should be taken into account while developing modern industrial biocatalysts based on inulinase.

Abstract Image

Abstract Image

黑曲霉内切酶与单糖、双糖和多糖相互作用的特异性
摘要 本研究旨在分析米曲霉内切酶分子与单糖、双糖和多糖结合后的空间组织特征。本研究考察了菊粉酶与单糖(葡萄糖、果糖)、双糖(蔗糖、甘露糖)和多糖(菊粉)结合后内腔体积和数量的变化。研究描述了隧道和孔的数量和长度的变化,分析了酶分子表面带电和疏水氨基酸残基簇的组成和定位的重组。研究表明,菊粉酶与蔗糖(替代底物)和甘露糖(激活剂)的复合物模型表现出相同类型的内部结构。在与果糖(反应产物)和葡萄糖(抑制剂)形成复合物时,也发现了类似的模式。此外,研究还证实,菊粉酶与单糖、二糖和多糖结合后,带电簇和疏水簇的化学成分都不会发生显著变化,也就是说,上述菊粉酶与碳水化合物之间的相互作用主要影响的是酶的内部结构。在开发基于菊粉酶的现代工业生物催化剂时,应考虑到菊粉酶与各种配体结合的特异性。
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来源期刊
Biophysics
Biophysics Biochemistry, Genetics and Molecular Biology-Biophysics
CiteScore
1.20
自引率
0.00%
发文量
67
期刊介绍: Biophysics is a multidisciplinary international peer reviewed journal that covers a wide scope of problems related to the main physical mechanisms of processes taking place at different organization levels in biosystems. It includes structure and dynamics of macromolecules, cells and tissues; the influence of environment; energy transformation and transfer; thermodynamics; biological motility; population dynamics and cell differentiation modeling; biomechanics and tissue rheology; nonlinear phenomena, mathematical and cybernetics modeling of complex systems; and computational biology. The journal publishes short communications devoted and review articles.
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