Breeding rice for yield improvement through CRISPR/Cas9 genome editing method: current technologies and examples

IF 3.4 3区 生物学 Q1 PLANT SCIENCES
Balakrishnan Rengasamy, Mrinalini Manna, Nargis Begum Thajuddin, Muthukrishnan Sathiyabama, Alok Krishna Sinha
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引用次数: 0

Abstract

The impending climate change is threatening the rice productivity of the Asian subcontinent as instances of crop failures due to adverse abiotic and biotic stress factors are becoming common occurrences. CRISPR-Cas9 mediated genome editing offers a potential solution for improving rice yield as well as its stress adaptation. This technology allows modification of plant’s genetic elements and is not dependent on foreign DNA/gene insertion for incorporating a particular trait. In this review, we have discussed various CRISPR-Cas9 mediated genome editing tools for gene knockout, gene knock-in, simultaneously disrupting multiple genes by multiplexing, base editing and prime editing the genes. The review here also presents how these genome editing technologies have been employed to improve rice productivity by directly targeting the yield related genes or by indirectly manipulating various abiotic and biotic stress responsive genes. Lately, many countries treat genome-edited crops as non-GMOs because of the absence of foreign DNA in the final product. Thus, genome edited rice plants with improved yield attributes and stress resilience are expected to be accepted by the public and solve food crisis of a major portion of the globe.

Abstract Image

通过 CRISPR/Cas9 基因组编辑方法培育水稻以提高产量:现有技术与实例
迫在眉睫的气候变化正威胁着亚洲次大陆的水稻生产力,因为不利的非生物和生物胁迫因素导致作物歉收的情况正变得屡见不鲜。CRISPR-Cas9 介导的基因组编辑技术为提高水稻产量及其胁迫适应性提供了一种潜在的解决方案。这项技术可以修改植物的遗传因子,不依赖外来 DNA/基因的插入来整合特定的性状。在这篇综述中,我们讨论了各种 CRISPR-Cas9 介导的基因组编辑工具,包括基因敲除、基因敲入、通过多路复用同时破坏多个基因、碱基编辑和基因素编辑。本综述还介绍了如何利用这些基因组编辑技术,通过直接针对产量相关基因或间接操纵各种非生物和生物胁迫响应基因来提高水稻产量。最近,许多国家将基因组编辑作物视为非转基因作物,因为最终产品中没有外来 DNA。因此,经过基因组编辑的水稻植物具有更好的产量属性和抗逆性,有望被公众接受,并解决全球大部分地区的粮食危机。
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来源期刊
CiteScore
7.10
自引率
0.00%
发文量
126
期刊介绍: Founded in 1995, Physiology and Molecular Biology of Plants (PMBP) is a peer reviewed monthly journal co-published by Springer Nature. It contains research and review articles, short communications, commentaries, book reviews etc., in all areas of functional plant biology including, but not limited to plant physiology, biochemistry, molecular genetics, molecular pathology, biophysics, cell and molecular biology, genetics, genomics and bioinformatics. Its integrated and interdisciplinary approach reflects the global growth trajectories in functional plant biology, attracting authors/editors/reviewers from over 98 countries.
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