A Raman Spectroscopic Study of the Conformation of Flavin Adenine Dinucleotide, a CoEnzyme of D-Amino Acid Oxidase

IF 4.033 Q4 Biochemistry, Genetics and Molecular Biology

Biophysics Pub Date : 2024-03-07 DOI:10.1134/S0006350923050068

J. V. Bochkova, W. Liu, N. A. Brazhe, A. A. Zhgun, G. V. Maksimov

引用次数: 0

Abstract

The spectra of surface-enhanced Raman scattering of D-amino acid oxidase from pig kidney were recorded and analyzed using silver nanoparticles; characteristic spectral parameters of changes in the conformation of the flavinadenine dinucleotide cofactor during activation of the enzyme by D-amino acids were revealed. It was found that the time during which changes in the conformation of the flavinadenine dinucleotide were recorded depended on the substrate specificity of the enzyme: in the presence of D-alanine, this took several seconds, and in the presence of D-serine, minutes.

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关于 D-氨基酸氧化酶辅酶黄素腺嘌呤二核苷酸构象的拉曼光谱研究

摘要利用纳米银颗粒记录和分析了猪肾中 D-氨基酸氧化酶的表面增强拉曼散射光谱，揭示了 D-氨基酸激活该酶过程中黄素腺嘌呤二核苷酸辅因子构象变化的特征光谱参数。研究发现，记录黄素腺嘌呤二核苷酸构象变化的时间取决于酶的底物特异性：在 D-丙氨酸存在的情况下，需要几秒钟，而在 D-丝氨酸存在的情况下，需要几分钟。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Biophysics Biochemistry, Genetics and Molecular Biology-Biophysics

CiteScore

1.20

自引率

0.00%

发文量

期刊介绍： Biophysics is a multidisciplinary international peer reviewed journal that covers a wide scope of problems related to the main physical mechanisms of processes taking place at different organization levels in biosystems. It includes structure and dynamics of macromolecules, cells and tissues; the influence of environment; energy transformation and transfer; thermodynamics; biological motility; population dynamics and cell differentiation modeling; biomechanics and tissue rheology; nonlinear phenomena, mathematical and cybernetics modeling of complex systems; and computational biology. The journal publishes short communications devoted and review articles.