Osteomacs promote maintenance of murine hematopoiesis through megakaryocyte-induced upregulation of Embigin and CD166.

IF 5.9 2区 医学 Q1 CELL & TISSUE ENGINEERING
Stem Cell Reports Pub Date : 2024-04-09 Epub Date: 2024-03-07 DOI:10.1016/j.stemcr.2024.02.004
Safa F Mohamad, Roy El Koussa, Joydeep Ghosh, Rachel Blosser, Andrea Gunawan, Justin Layer, Chi Zhang, Sonali Karnik, Utpal Davé, Melissa A Kacena, Edward F Srour
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引用次数: 0

Abstract

Maintenance of hematopoietic stem cell (HSC) function in the niche is an orchestrated event. Osteomacs (OM) are key cellular components of the niche. Previously, we documented that osteoblasts, OM, and megakaryocytes interact to promote hematopoiesis. Here, we further characterize OM and identify megakaryocyte-induced mediators that augment the role of OM in the niche. Single-cell mRNA-seq, mass spectrometry, and CyTOF examination of megakaryocyte-stimulated OM suggested that upregulation of CD166 and Embigin on OM augment their hematopoiesis maintenance function. CD166 knockout OM or shRNA-Embigin knockdown OM confirmed that the loss of these molecules significantly reduced the ability of OM to augment the osteoblast-mediated hematopoietic-enhancing activity. Recombinant CD166 and Embigin partially substituted for OM function, characterizing both proteins as critical mediators of OM hematopoietic function. Our data identify Embigin and CD166 as OM-regulated critical components of HSC function in the niche and potential participants in various in vitro manipulations of stem cells.

骨刺通过巨核细胞诱导的 Embigin 和 CD166 上调促进小鼠造血功能的维持。
造血干细胞(HSC)在生态位中的功能维持是一个协调的过程。成骨细胞(OM)是骨龛的关键细胞成分。此前,我们记录了成骨细胞、OM和巨核细胞相互作用促进造血的过程。在这里,我们进一步描述了OM的特征,并确定了巨核细胞诱导的介质,这些介质增强了OM在生态位中的作用。对巨核细胞刺激的OM进行的单细胞mRNA-seq、质谱分析和CyTOF检查表明,CD166和Embigin在OM上的上调增强了它们的造血维持功能。CD166敲除OM或shRNA-Embigin敲除OM证实,这些分子的缺失显著降低了OM增强成骨细胞介导的造血增强活性的能力。重组 CD166 和 Embigin 可部分替代 OM 的功能,这两种蛋白是 OM 造血功能的关键介质。我们的数据确定了Embigin和CD166是OM调节的干细胞功能的关键成分,也是干细胞各种体外操作的潜在参与者。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Stem Cell Reports
Stem Cell Reports CELL & TISSUE ENGINEERING-CELL BIOLOGY
CiteScore
10.50
自引率
1.70%
发文量
200
审稿时长
28 weeks
期刊介绍: Stem Cell Reports publishes high-quality, peer-reviewed research presenting conceptual or practical advances across the breadth of stem cell research and its applications to medicine. Our particular focus on shorter, single-point articles, timely publication, strong editorial decision-making and scientific input by leaders in the field and a "scoop protection" mechanism are reasons to submit your best papers.
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