Monitoring redox stress in human airway epithelial cells exposed to woodsmoke at an air-liquid interface.

IF 7.2 1区 医学 Q1 TOXICOLOGY
Aiman Abzhanova, Jon Berntsen, Edward R Pennington, Lisa Dailey, Syed Masood, Ingrid George, Nina Warren, Joseph Martin, Michael D Hays, Andrew J Ghio, Jason P Weinstein, Yong Ho Kim, Earl Puckett, James M Samet
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引用次数: 0

Abstract

Wildland fires contribute significantly to the ambient air pollution burden worldwide, causing a range of adverse health effects in exposed populations. The toxicity of woodsmoke, a complex mixture of gases, volatile organic compounds, and particulate matter, is commonly studied in vitro using isolated exposures of conventionally cultured lung cells to either resuspended particulate matter or organic solvent extracts of smoke, leading to incomplete toxicity evaluations. This study aimed to improve our understanding of the effects of woodsmoke inhalation by building an advanced in vitro exposure system that emulates human exposure of the airway epithelium. We report the development and characterization of an innovative system that permits live-cell monitoring of the intracellular redox status of differentiated primary human bronchial epithelial cells cultured at an air-liquid interface (pHBEC-ALI) as they are exposed to unfractionated woodsmoke generated in a tube furnace in real time. pHBEC-ALI exposed to freshly generated woodsmoke showed oxidative changes that were dose-dependent and reversible, and not attributable to carbon monoxide exposure. These findings show the utility of this novel system for studying the molecular initiating events underlying woodsmoke-induced toxicity in a physiologically relevant in vitro model, and its potential to provide biological plausibility for risk assessment and public health measures.

监测暴露在空气-液体界面木烟中的人体气道上皮细胞的氧化还原压力。
野外火灾严重加剧了全球环境空气污染,对暴露人群的健康造成了一系列不利影响。木烟是一种由气体、挥发性有机化合物和颗粒物组成的复杂混合物,对其毒性的体外研究通常采用将传统培养的肺细胞分离暴露于重悬浮颗粒物或烟雾的有机溶剂提取物的方法,这导致了不完整的毒性评估。本研究旨在通过建立一个先进的体外暴露系统来模拟人类气道上皮细胞的暴露,从而提高我们对吸入木烟影响的认识。我们报告了一种创新系统的开发和表征,该系统允许活细胞监测在空气-液体界面培养的分化原代人类支气管上皮细胞(pHBEC-ALI)在实时暴露于管式炉中产生的未分馏木烟时的细胞内氧化还原状态。这些研究结果表明,这种新型系统可以在与生理相关的体外模型中研究木烟诱导毒性的分子起始事件,并有可能为风险评估和公共卫生措施提供生物学可信性。
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来源期刊
CiteScore
15.90
自引率
4.00%
发文量
69
审稿时长
6 months
期刊介绍: Particle and Fibre Toxicology is an online journal that is open access and peer-reviewed. It covers a range of disciplines such as material science, biomaterials, and nanomedicine, focusing on the toxicological effects of particles and fibres. The journal serves as a platform for scientific debate and communication among toxicologists and scientists from different fields who work with particle and fibre materials. The main objective of the journal is to deepen our understanding of the physico-chemical properties of particles, their potential for human exposure, and the resulting biological effects. It also addresses regulatory issues related to particle exposure in workplaces and the general environment. Moreover, the journal recognizes that there are various situations where particles can pose a toxicological threat, such as the use of old materials in new applications or the introduction of new materials altogether. By encompassing all these disciplines, Particle and Fibre Toxicology provides a comprehensive source for research in this field.
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