Convenient pre-separation method depends on negative charges suitable for glycan sequencing analysis.

IF 1.4 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY

Bioscience, Biotechnology, and Biochemistry Pub Date : 2024-05-22 DOI:10.1093/bbb/zbae030

Shigemitsu Segawa, Shunji Natsuka

引用次数: 0

Abstract

For precise structural analysis of complex glycans, it is necessary to fractionate glycans based on acidity prior to liquid chromatography-mass spectrometry analysis. Conventional fractionation using high-performance liquid chromatography is time consuming and not high throughput. Therefore, we developed a rapid method for the fractionation using a small cartridge column. This enables rapid multi-sample processing for glycan sequencing.

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方便的预分离方法取决于负电荷，适用于糖测序分析。

为了对复杂的聚糖进行精确的结构分析，有必要在液相色谱-质谱（LC-MS）分析之前根据酸度对聚糖进行分馏。传统的高效液相色谱法（HPLC）分馏耗时且通量不高。因此，我们开发了一种使用小型滤芯柱进行快速分馏的方法。这样就能快速处理多个样品，进行聚糖测序。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bioscience, Biotechnology, and Biochemistry 生物-生化与分子生物学

CiteScore

3.50

自引率

0.00%

发文量

183

审稿时长

1 months

期刊介绍： Bioscience, Biotechnology, and Biochemistry publishes high-quality papers providing chemical and biological analyses of vital phenomena exhibited by animals, plants, and microorganisms, the chemical structures and functions of their products, and related matters. The Journal plays a major role in communicating to a global audience outstanding basic and applied research in all fields subsumed by the Japan Society for Bioscience, Biotechnology, and Agrochemistry (JSBBA).