Oviduct and endometrial epithelium improve in vitro produced bovine embryo developmental kinetics.

IF 3.7 3区生物学 Q1 DEVELOPMENTAL BIOLOGY

Reproduction Pub Date : 2024-04-17 Print Date: 2024-05-01 DOI:10.1530/REP-24-0008

L Kirsten Senn, Katheryn D Peterson, J Lannett Edwards, Rebecca R Payton, Daniel J Mathew

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引用次数: 0

Abstract

In brief: Standard in vitro produced (IVP) bovine embryo culture media limit embryonic development. Culturing IVP bovine embryos in standard IVP bovine embryo culture media conditioned with oviduct and/or endometrial cells improves blastocyst formation and reduces the time to formation.

Abstract: In vitro embryo production in cattle greatly impacts blastomere biochemistry, embryo rate of development and pre- and post-transfer survival. In vivo, the bovine embryo migrates through the oviduct isthmus before entering the uterus on approximately day 4 of development where it remains unattached within the uterine lumen until day 20 of gestation. During this time, the embryo is sequentially exposed to oviduct followed by endometrial secretions that support embryonic development. Considering this, we tested the effect of culturing in vitro produced (IVP) bovine embryos sequentially in oviduct epithelial- (OEp; days 1-3) followed by endometrial epithelial- (EEp) or EEp and fibroblast cell (EEp/F; days 4-8)-conditioned media on embryonic development using a time-lapse monitoring system. Compared to control, culturing IVP embryos in EEp- or EEp/F-conditioned media without prior culture in OEp-conditioned media increased blastocyst formation (P < 0.05) and reduced the time to blastocyst formation (P < 0.05). Culturing IVP bovine embryos in OEp-conditioned media followed by EEp- or EEp/F-conditioned media, however, had the greatest impact on embryo developmental kinetics and increased morula and blastocyst formation (P < 0.05) and reduced time to formation (P < 0.05). Day 8 blastocyst cell numbers, diameter and quality were not significantly different, although, blastocyst quality scores were less (indicative of better quality) for all cell-conditioned media compared to control. In conclusion, IVP bovine embryo development may be improved using a sequential embryo culture system involving bovine oviduct followed by endometrial cell-conditioned media.

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输卵管和子宫内膜上皮可改善体外培育牛胚胎的发育动力学。

牛的体外胚胎生产对胚泡生物化学、胚胎发育速度和移植前后的存活率有很大影响。在体内，牛胚胎通过输卵管峡部移行，大约在发育的第 4 天进入子宫，在妊娠的第 20 天之前，胚胎在子宫腔内一直保持未附着状态。在此期间，胚胎依次接触输卵管和子宫内膜分泌物，这些分泌物支持胚胎发育。有鉴于此，我们使用延时监测系统测试了在输卵管上皮（OEp；第 1-3 天）、子宫内膜上皮（EEp）或 EEp 和成纤维细胞（EEp/F；第 4-8 天）条件培养基中依次培养体外生产（IVP）牛胚胎对胚胎发育的影响。与对照组相比，在 EEp 或 EEp/F 条件培养基中培养 IVP 胚胎而不事先在 OEp 条件培养基中培养，可增加囊胚形成（P < 0.05）并缩短囊胚形成时间（P < 0.05）。然而，先用 OEp 条件培养基培养 IVP 牛胚胎，再用 EEp 或 EEp/F 条件培养基培养 IVP 牛胚胎，对胚胎发育动力学的影响最大，可增加胚泡和囊胚的形成（P < 0.05），缩短胚泡形成时间（P < 0.05）。与对照组相比，第 8 天囊胚细胞数量、直径和质量没有显著差异，但所有细胞条件培养基的囊胚质量评分都较低（表明质量较好）。总之，使用包括牛输卵管和子宫内膜细胞条件培养基的连续胚胎培养系统可改善 IVP 牛胚胎发育。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Reproduction 生物-发育生物学

CiteScore

7.40

自引率

2.60%

发文量

199

审稿时长

4-8 weeks

期刊介绍： Reproduction is the official journal of the Society of Reproduction and Fertility (SRF). It was formed in 2001 when the Society merged its two journals, the Journal of Reproduction and Fertility and Reviews of Reproduction. Reproduction publishes original research articles and topical reviews on the subject of reproductive and developmental biology, and reproductive medicine. The journal will consider publication of high-quality meta-analyses; these should be submitted to the research papers category. The journal considers studies in humans and all animal species, and will publish clinical studies if they advance our understanding of the underlying causes and/or mechanisms of disease. Scientific excellence and broad interest to our readership are the most important criteria during the peer review process. The journal publishes articles that make a clear advance in the field, whether of mechanistic, descriptive or technical focus. Articles that substantiate new or controversial reports are welcomed if they are noteworthy and advance the field. Topics include, but are not limited to, reproductive immunology, reproductive toxicology, stem cells, environmental effects on reproductive potential and health (eg obesity), extracellular vesicles, fertility preservation and epigenetic effects on reproductive and developmental processes.