NONDOMESTIC FELID ABC BLOOD PHENOTYPING, GENOTYPING, AND CROSSMATCHING.

IF 0.7 4区农林科学 Q3 VETERINARY SCIENCES

Journal of Zoo and Wildlife Medicine Pub Date : 2024-03-01 DOI:10.1638/2023-0025

Lily Parkinson, Henrike Kuder, Alexandra Kehl, Amie Koenig, Jennifer Good, Jennifer Hausmann, Urs Giger

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引用次数: 0

Abstract

Based upon previous clinical experience with domestic cats (Felis catus), the ability to assess ABC blood types and blood (in-)compatibilities of nondomestic felids, and adequately consider and plan for blood transfusions, may be important. Although nondomestic felids appear to have an ABC blood group system similar to domestic cats, typing with point-of-care kits and by CMAH genotyping for domestic cats have not been reported. In this study, 162 blood samples from 18 different nondomestic felid species (cheetah [Acinonyx jubatus, n = 42], lion [Panthera leo, n = 33], tiger [Panthera tigris, n = 23], Canada lynx [Lynx canadensis, n = 11], snow leopard [Uncia uncia, n = 10], puma [Puma concolor, n = 7], clouded leopard [Neofelis nebulosa, n = 6], serval [Leptailurus serval, n = 5], jaguar [Panthera onca, n = 5], fishing cat [Prionailurus viverrinus, n = 4], Pallas cat [Felis manul, n = 3], bobcat [Lynx rufus, n = 3], ocelot [Leopardus pardalis, n = 3], black footed cat [Felis nigripes, n = 2], leopard [Panthera pardus, n = 2], African wildcat [Felis lybica, n = 1], caracal [Caracal caracal, n = 1], and sand cat [Felis margarita, n = 1]) were ABC blood typed by laboratory and point-of-care tests, genotyped for four known CMAH variants for type B and type C (AB) phenotypes, and crossmatched with one another and domestic type A cats. Traditional tube typing identified blood type A (n = 106), type B (n = 8), type C (n = 43), and no discernible ABC type (n = 4). Several discrepancies were found between point-of-care and traditional typing test results. None of the tested felids possessed the four CMAH variants responsible for type B and C (AB) in domestic cats. Crossmatch incompatibilities (≥2+ agglutination) were identified within and between nondomestic felid species and beyond ABC incompatibilities. Of 26 crossmatches performed between domestic cats and various nondomestic felids, only 7 (27%) were compatible. In conclusion, point-of-care typing kits and CMAH genotyping, successfully used in domestic cats, may not identify the correct ABC blood type in nondomestic felids. Prior crossmatching is recommended to increase the likelihood of compatible transfusions between any nondomestic felids.

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非家养猫科动物 abc 血液表型、基因分型和交叉配对。

根据以往对家猫（Felis catus）的临床经验，评估非家养猫科动物的 ABC 血型和血液（不）相容性以及充分考虑和计划输血的能力可能非常重要。尽管非家养猫科动物的 ABC 血型系统似乎与家猫相似，但目前还没有关于家猫用护理点试剂盒和 CMAH 基因分型进行分型的报道。在这项研究中，162 份血液样本来自 18 种不同的非家养猫科动物（猎豹 [Acinonyx jubatus, n = 42]、狮子 [Panthera leo, n = 33]、老虎 [Panthera tigris, n = 23]、加拿大猞猁 [Lynx canadensis, n = 11]、雪豹 [Uncia uncia、n = 10]、美洲狮[Puma concolor，n = 7]、云豹[Neofelis nebulosa，n = 6]、薮猫[Leptailurus serval，n = 5]、美洲虎[Panthera onca，n = 5]、渔猫[Prionailurus viverrinus，n = 4]、帕拉斯猫[Felis manul、n = 3]、山猫[Lynx rufus，n = 3]、猫鼬[Leopardus pardalis，n = 3]、黑脚猫[Felis nigripes，n = 2]、豹[Panthera pardus，n = 2]、非洲野猫[Felis lybica，n = 1]、狞猫[Caracal caracal，n = 1]、和沙猫[Felis margarita, n = 1])通过实验室和护理点检测进行了 ABC 血型鉴定，对四种已知的 B 型和 C 型（AB）表型的 CMAH 变体进行了基因分型，并与其他猫和家养 A 型猫进行了交叉配对。传统的试管分型确定了 A 型血 (106 只)、B 型血 (8 只)、C 型血 (43 只)，没有发现 ABC 型血 (4 只)。在护理点检测和传统分型检测结果之间发现了一些差异。所有接受测试的猫科动物都不具备导致家猫B型和C型（AB型）的四种CMAH变体。在非家养猫科动物物种内和物种间发现了交叉配型不相容（≥2+凝集），而且超出了ABC不相容的范围。在家猫和各种非家猫类之间进行的 26 次交叉配型中，只有 7 次（27%）是相容的。总之，成功用于家猫的护理点分型试剂盒和 CMAH 基因分型可能无法识别非家养猫科动物的正确 ABC 血型。建议事先进行交叉配型，以提高非家养猫科动物之间输血相合的可能性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Zoo and Wildlife Medicine 农林科学-兽医学

CiteScore

1.70

自引率

14.30%

发文量

审稿时长

9-24 weeks

期刊介绍： The Journal of Zoo and Wildlife Medicine (JZWM) is considered one of the major sources of information on the biology and veterinary aspects in the field. It stems from the founding premise of AAZV to share zoo animal medicine experiences. The Journal evolved from the long history of members producing case reports and the increased publication of free-ranging wildlife papers. The Journal accepts manuscripts of original research findings, case reports in the field of veterinary medicine dealing with captive and free-ranging wild animals, brief communications regarding clinical or research observations that may warrant publication. It also publishes and encourages submission of relevant editorials, reviews, special reports, clinical challenges, abstracts of selected articles and book reviews. The Journal is published quarterly, is peer reviewed, is indexed by the major abstracting services, and is international in scope and distribution. Areas of interest include clinical medicine, surgery, anatomy, radiology, physiology, reproduction, nutrition, parasitology, microbiology, immunology, pathology (including infectious diseases and clinical pathology), toxicology, pharmacology, and epidemiology.