Correlation analysis of cancer stem cell marker CD133 and human endogenous retrovirus (HERV)-K env in SKOV3 ovarian cancer cells.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-04-01 Epub Date: 2024-03-08 DOI:10.1007/s13258-024-01499-6

Do-Ye Kim, Heungyeol Kim, Eun-Ji Ko, Suk Bong Koh, Hongbae Kim, Ji Young Lee, Chul Min Lee, Wan Kyu Eo, Ki Hyung Kim, Hee-Jae Cha

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Abstract

Background: Human endogenous retrovirus (HERV)-K is a type of retrovirus that is present in the human genome, and its expression is usually silenced in healthy tissues. The precise mechanism by which HERV-K env influences cancer stemness is not fully understood, but it has been suggested that HERV-K env may activate various signaling pathways that promote stemness traits in cancer cells.

Objective: To establish the connection between HERV-K env expression and cancer stemness in ovarian cancer cells, we carried out correlation analyses between HERV-K env and the cancer stem cell (CSC) marker known as the cluster of differentiation 133 (CD133) gene in SKOV3 ovarian cancer cells.

Method: To perform correlation analysis between HERV-K env and CSCs, ovarian cancer cells were cultured in a medium designed for cancer stem cell induction. The expression of HERV-K env and CD133 genes was verified using quantitative real-time polymerase chain reaction (RT-qPCR) and Western blot analyses. Additionally, the expression of stemness-related markers, such as OCT-4 and Nanog, was also confirmed using RT-qPCR.

Results: In the stem cell induction medium, the number of tumorsphere-type SKOV3 cells increased, and the expression of CD133 and HERV-K env genes was up-regulated. Additionally, other stemness-related markers like OCT-4 and Nanog also exhibited increased expression when cultured in the cancer stem cell induction medium. However, when HERV-K env knockout (KO) SKOV3 cells were cultured in the same cancer stem cell induction medium, there was a significant decrease in the number of tumorsphere-type cells compared to mock SKOV3 cells subjected to the same conditions. Furthermore, the expression of CD133, Nanog, and OCT-4 did not show a significant increase in HERV-K env KO SKOV3 cells compared to mock SKOV3 cells cultured in the same cancer stem cell induction medium.

Conclusion: These findings indicate that the expression of HERV-K env increased in SKOV3 cells when cultured in cancer stem cell induction media, and cancer stem cell induction was inhibited by KO of HERV-K env in SKOV3 cells. These results suggest a strong association between HERV-K env and stemness in SKOV3 ovarian cancer cells.

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SKOV3 卵巢癌细胞中癌症干细胞标记 CD133 与人类内源性逆转录病毒 (HERV)-K env 的相关性分析。

背景：人类内源性逆转录病毒（HERV）-K是存在于人类基因组中的一种逆转录病毒，其表达在健康组织中通常处于沉默状态。HERV-K env影响癌症干性的确切机制尚不完全清楚，但有研究认为，HERV-K env可能会激活促进癌细胞干性特征的各种信号通路：为了确定 HERV-K env 表达与卵巢癌细胞中癌症干性之间的联系，我们对 SKOV3 卵巢癌细胞中的 HERV-K env 与癌症干细胞（CSC）标志物分化簇 133（CD133）基因进行了相关性分析：为了进行 HERV-K env 与癌症干细胞之间的相关性分析，卵巢癌细胞在专为诱导癌症干细胞而设计的培养基中进行培养。采用实时定量聚合酶链反应（RT-qPCR）和 Western 印迹分析验证 HERV-K env 和 CD133 基因的表达。此外，还使用实时聚合酶链反应（RT-qPCR）确认了干细胞相关标记物（如 OCT-4 和 Nanog）的表达：结果：在干细胞诱导培养基中，瘤球型SKOV3细胞数量增加，CD133和HERV-K env基因表达上调。此外，在癌症干细胞诱导培养基中培养时，OCT-4 和 Nanog 等其他干细胞相关标记物的表达也有所增加。然而，当HERV-K env基因敲除（KO）的SKOV3细胞在相同的癌症干细胞诱导培养基中培养时，与模拟SKOV3细胞相比，肿瘤球型细胞的数量显著减少。此外，与在相同的癌症干细胞诱导培养基中培养的模拟SKOV3细胞相比，HERV-K env KO SKOV3细胞中CD133、Nanog和OCT-4的表达没有明显增加：这些研究结果表明，在癌症干细胞诱导培养基中培养的SKOV3细胞中，HERV-K env的表达增加，而在SKOV3细胞中KO HERV-K env可抑制癌症干细胞诱导。这些结果表明，在SKOV3卵巢癌细胞中，HERV-K env与干性密切相关。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.