Human milk-specific fat components enhance the secretion of ghrelin by MGN3-1 cells.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-05-22 DOI:10.1093/bbb/zbae028

Kentaro Kaneko, Eriko Taniguchi, Yui Funatsu, Yoshitaka Nakamura, Hiroshi Iwakura, Kousaku Ohinata

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Abstract

Triacylglycerols (TAGs) are a major fat component in human milk. Since gastric lipase produces 1,2-diacylglycerol from TAGs, we focused on the bioactivity of human milk-derived diacylglycerols in stomach cells. Ghrelin is produced in the stomach and acts as an important regulator of growth hormone secretion and energy homeostasis. In this study, we showed that 1-oleoyl-2-palmitoylglycerol (OP) increased ghrelin secretion, whereas 1,3-dioleoyl-2-palmitoylglycerol (OPO), a major component of human milk TAGs, did not increase ghrelin secretion in the ghrelin-secreting cell line, MGN3-1. Therefore, diacylglycerol OP may directly contribute to the regulation of ghrelin secretion. We also found that 2-palmitoylglycerol and 1- and 2-oleoylglycerol increased ghrelin secretion. Finally, we demonstrated that intracellular cAMP levels and preproghrelin and ghrelin O-acyl transferase expression levels were enhanced by OP treatment in MGN3-1 cells. This may represent an example of a novel mother-infant interaction mediated by fat components derived from human breast milk.

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人乳特异性脂肪成分会增强 MGN3-1 细胞分泌胃泌素的能力。

三酰甘油（TAG）是母乳中的主要脂肪成分。由于胃脂肪酶会从 TAGs 中产生 1,2-二酰甘油，因此我们重点研究了源于母乳的二酰甘油在胃细胞中的生物活性。胃泌素在胃中产生，是生长激素分泌和能量平衡的重要调节因子。在这项研究中，我们发现 1-油酰基-2-棕榈酰甘油（OP）能增加胃泌素的分泌，而 1,3-二油酰基-2-棕榈酰甘油（OPO）（人乳 TAGs 的主要成分）并不能增加胃泌素分泌细胞系 MGN3-1 的胃泌素水平。因此，二酰甘油 OP 可能直接参与了胃泌素分泌的调节。我们还发现，2-棕榈酰甘油、1-和 2-油酰甘油可增加胃泌素的分泌。最后，我们证实 OP 处理可提高 MGN3-1 细胞的细胞内 cAMP 水平以及前胃泌素和胃泌素 O-酰基转移酶的表达水平。这可能代表了一种由母乳中的脂肪成分介导的新型母婴相互作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.