Characterization of microfluidic trap and mixer module for rapid fluorescent tagging of microplastics

IF 2.3 4区 工程技术 Q2 INSTRUMENTS & INSTRUMENTATION
Seongcheol Shin, Boeun Jeon, Wonkyu Kang, Cholong Kim, Jonghoon Choi, Sung Chul Hong, Hyun Ho Lee
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Abstract

This study introduces a practical approach utilizing microfluidic trap and mixer modules fabricated with polydimethylsiloxane (PDMS) microfluidic devices. These modules were employed to capture and fluorescently label various randomly shaped microplastics (MPs) like polyethylene (PE), polypropylene (PP), and polystyrene (PS). Within the MPs trap module, grooves were incorporated into a straight-lined channel using SU-8 photolithography. This design induced turbulence effectively trapping and gathering the MPs within aqueous phases at 15 groove spaces, which achieved a trapping efficiency of up to 69% for PS MPs sized at a flow rate of 2 mL/min. Additionally, a mixer module featuring two flow inlets was designed to create a serpentine microfluidic channel, whose design significantly reduced sample and reagent (Nile Red) consumption during MP fluorescence staining at 80 °C. Furthermore, 2 nm gold nanoparticles (Au NPs), conjugated with a PS binding peptide (PSBP), were examined as an alternative fluorescent agent at room temperature. Photoluminescence (PL) and Fourier transform infrared (FT-IR) showcased efficiency of mixer module in labeling 30 mL MP solutions within a short time of 15 min. Moreover, a combined platform integrating trap and mixer devices was devised, incorporating a disposable heating pad and filter paper unit, which offers a simplified and compact MPs staining tool including spherical PE nanoplastics (200 nm–99 μm). This study aims to propose a preliminary concept for a lab-on-a-chip, facilitating the simultaneous collection and fluorescent labeling, which can be instrumentally implemented in future MPs monitoring.

用于快速荧光标记微塑料的微流体捕集器和混合器模块的特性分析
摘要 本研究介绍了一种利用聚二甲基硅氧烷(PDMS)微流体装置制造的微流体捕集器和混合器模块的实用方法。这些模块用于捕获和荧光标记各种随机形状的微塑料(MPs),如聚乙烯(PE)、聚丙烯(PP)和聚苯乙烯(PS)。在 MPs 捕集模块中,使用 SU-8 光刻技术在直行通道中加入了凹槽。这种设计可在 15 个沟槽间隙处产生湍流,有效地捕获和聚集水相中的 MPs,在流速为 2 mL/min 的情况下,PS MPs 的捕获效率高达 69%。此外,还设计了一个具有两个流量入口的混合器模块,以创建一个蛇形微流控通道,其设计大大减少了在 80 °C 下对 MP 进行荧光染色时的样品和试剂(尼罗河红)消耗。此外,还研究了室温下与 PS 结合肽(PSBP)共轭的 2 nm 金纳米粒子(Au NPs)作为替代荧光剂。光致发光(PL)和傅立叶变换红外(FT-IR)显示了混合器模块在 15 分钟的短时间内标记 30 mL MP 溶液的效率。此外,还设计了一个集成了捕集器和混合器装置的组合平台,其中包含一个一次性加热垫和滤纸装置,提供了一个简化、紧凑的 MPs 染色工具,包括球形聚乙烯纳米塑料(200 nm-99 μm)。本研究旨在提出片上实验室的初步概念,便于同时进行收集和荧光标记,可在未来的 MPs 监测中使用。
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来源期刊
Microfluidics and Nanofluidics
Microfluidics and Nanofluidics 工程技术-纳米科技
CiteScore
4.80
自引率
3.60%
发文量
97
审稿时长
2 months
期刊介绍: Microfluidics and Nanofluidics is an international peer-reviewed journal that aims to publish papers in all aspects of microfluidics, nanofluidics and lab-on-a-chip science and technology. The objectives of the journal are to (1) provide an overview of the current state of the research and development in microfluidics, nanofluidics and lab-on-a-chip devices, (2) improve the fundamental understanding of microfluidic and nanofluidic phenomena, and (3) discuss applications of microfluidics, nanofluidics and lab-on-a-chip devices. Topics covered in this journal include: 1.000 Fundamental principles of micro- and nanoscale phenomena like, flow, mass transport and reactions 3.000 Theoretical models and numerical simulation with experimental and/or analytical proof 4.000 Novel measurement & characterization technologies 5.000 Devices (actuators and sensors) 6.000 New unit-operations for dedicated microfluidic platforms 7.000 Lab-on-a-Chip applications 8.000 Microfabrication technologies and materials Please note, Microfluidics and Nanofluidics does not publish manuscripts studying pure microscale heat transfer since there are many journals that cover this field of research (Journal of Heat Transfer, Journal of Heat and Mass Transfer, Journal of Heat and Fluid Flow, etc.).
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