The Evaluation of Anti-Osteoclastic Activity of the Novel Calcium Hydroxide Biodegradable Nanoparticles as an Intracanal Medicament

IF 3.5 2区医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of endodontics Pub Date : 2024-05-01 DOI:10.1016/j.joen.2024.02.023

Patarawadee Promta DDS , Patcharaporn Chaiyosang DDS , Aussara Panya PhD , Pongrapee Laorodphun MS , Warat Leelapornpisid DDS, PhD , Napatsorn Imerb DDS, PhD

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Abstract

Introduction

The aim of this study was to evaluate the anti-osteoclastic activity of calcium hydroxide–loaded poly(lactic-co-glycolic acid) nanoparticles [Ca(OH)₂-loaded PLGA NPs] in comparison to calcium hydroxide nanoparticles [Ca(OH)₂ NPs].

Methods

RAW 264.7 cell lines (third–fifth passage) were cultured and incubated with soluble receptor activator of nuclear factor kappa B ligand in triplicate. Subsequently, Ca(OH)₂-loaded PLGA NPs and Ca(OH)₂ NPs were added for 7 days to evaluate their effects on receptor activator of nuclear factor kappa B ligand–induced osteoclast differentiation of RAW 264.7 cells by tartrate-resistant acid phosphatase activity. Additionally, a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was conducted to confirm the cytotoxicity of treatments to cells.

Results

Tartrate-resistant acid phosphatase staining showed a significant reduction in the osteoclast number when treated with Ca(OH)₂-loaded PLGA NPs compared with Ca(OH)₂ NPs (P < .01). In comparison to the control, the number of osteoclasts significantly reduced upon treatment with Ca(OH)₂-loaded PLGA NPs (P < .05), but there was no significant difference in Ca(OH)₂ NPs. Furthermore, osteoclast morphology in both treatment groups exhibited smaller sizes than the control group. Neither Ca(OH)₂-loaded PLGA NPs nor Ca(OH)₂ NPs demonstrated cytotoxic effects on RAW264.7 cells.

Conclusions

Both Ca(OH)₂ NPs with and without poly(lactic-co-glycolic acid) have the ability to inhibit osteoclast differentiation. However, Ca(OH)₂-loaded PLGA NPs exhibit greater potential than Ca(OH)₂ NPs, making them a promising intracanal medicament for cases of root resorption.

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新型氢氧化钙生物可降解纳米颗粒作为龋内药物的抗骨质疏松活性评价。

简介本研究旨在评估氢氧化钙负载聚（乳酸-共聚-乙酸）纳米颗粒（Ca(OH)2-loaded PLGA NPs）与氢氧化钙纳米颗粒（Ca(OH)2 NPs）的抗骨质疏松活性：方法：培养 RAW 264.7 细胞株（第三-第五周期），并与可溶性核因子κB 配体受体激活剂（RANKL）进行三重培养。随后，加入 Ca(OH)2-loaded PLGA NPs 和 Ca(OH)2 NPs 7 天，通过耐酒石酸磷酸酶（TRAP）活性评估它们对 RANKL 诱导的 RAW 264.7 细胞破骨细胞分化的影响。此外，还进行了 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基溴化四唑（MTT）试验，以确认处理对细胞的细胞毒性：结果：TRAP染色显示，与Ca(OH)2 NPs（p2-负载的PLGA NPs（p2 NPs）相比，Ca(OH)2 NPs（p2-负载的PLGA NPs）处理的破骨细胞数量明显减少。此外，两个处理组的破骨细胞形态都比对照组小。负载 Ca(OH)2 的 PLGA NPs 和 Ca(OH)2 NPs 对 RAW264.7 细胞都没有细胞毒性作用：结论：含PLGA和不含PLGA的Ca（OH）2 NPs都具有抑制破骨细胞分化的能力。结论：含钙（OH）2 NPs 和不含钙（OH）2 NPs 的 PLGA 都具有抑制破骨细胞分化的能力，但含钙（OH）2 NPs 的 PLGA NPs 比含钙（OH）2 NPs 具有更大的潜力，因此是治疗牙根吸收病例的一种很有前途的根管内药物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of endodontics 医学-牙科与口腔外科

CiteScore

8.80

自引率

9.50%

发文量

224

审稿时长

42 days

期刊介绍： The Journal of Endodontics, the official journal of the American Association of Endodontists, publishes scientific articles, case reports and comparison studies evaluating materials and methods of pulp conservation and endodontic treatment. Endodontists and general dentists can learn about new concepts in root canal treatment and the latest advances in techniques and instrumentation in the one journal that helps them keep pace with rapid changes in this field.

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