The delayed effect of rotenone on the relative content of brain isatin-binding proteins of rats with experimental parkinsonism.

Q3 Biochemistry, Genetics and Molecular Biology
O A Buneeva, I G Kapitsa, L Sh Kazieva, N E Vavilov, V G Zgoda, A E Medvedev
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Abstract

Isatin (indoldione-2,3) is an endogenous biological regulator found in the brain, peripheral tissues, and biological fluids of humans and animals. Its biological activity is realized via isatin-binding proteins, many of which were identified during proteomic profiling of the brain of mice and rats. A number of these proteins are related to the development of neurodegenerative diseases. Previously, using a model of experimental Parkinsonism induced by a seven-day course of rotenone injections, we have observed behavioral disturbances, as well as changes in the profile and relative content of brain isatin-binding proteins. In this study, we have investigated behavioral responses and the relative content of brain isatin-binding proteins in rats with rotenone-induced Parkinsonism 5 days after the last administration of this neurotoxin. Despite the elimination of rotenone, animals exhibited motor and coordination impairments. Proteomic profiling of isatin-binding proteins revealed changes in the relative content of 120 proteins (the relative content of 83 proteins increased and that of 37 proteins decreased). Comparison of isatin-binding proteins characterized by the changes in the relative content observed in the brain right after the last injection of rotenone (n=16) and 5 days later (n=11) revealed only two common proteins (glyceraldehyde-3-phosphate dehydrogenase and subunit B of V-type proton ATPase). However, most of these proteins are associated with neurodegeneration, including Parkinson's and Alzheimer's diseases.

鱼藤酮对实验性帕金森病大鼠脑内异汀结合蛋白相对含量的延迟效应
伊沙替丁(吲哚二酮-2,3)是一种内源性生物调节剂,存在于人类和动物的大脑、外周组织和生物液体中。其生物活性是通过伊沙替丁结合蛋白实现的,其中许多蛋白是在小鼠和大鼠大脑蛋白质组分析过程中发现的。其中一些蛋白质与神经退行性疾病的发展有关。此前,我们曾利用注射鱼藤酮七天诱导的实验性帕金森病模型,观察到行为紊乱以及大脑中异汀结合蛋白的特征和相对含量的变化。在本研究中,我们对最后一次注射鱼藤酮诱发帕金森氏症大鼠5天后的行为反应和脑异丁烯结合蛋白的相对含量进行了调查。尽管消除了鱼藤酮,动物仍表现出运动和协调障碍。伊沙丁结合蛋白的蛋白质组分析表明,120种蛋白质的相对含量发生了变化(83种蛋白质的相对含量增加,37种蛋白质的相对含量减少)。根据最后一次注射鱼藤酮后(16 个)和 5 天后(11 个)在大脑中观察到的相对含量变化来比较伊沙替丁结合蛋白,发现只有两种常见蛋白(甘油醛-3-磷酸脱氢酶和 V 型质子 ATP 酶 B 亚基)。然而,这些蛋白质大多与神经变性有关,包括帕金森氏症和阿尔茨海默氏症。
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来源期刊
Biomeditsinskaya khimiya
Biomeditsinskaya khimiya Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)
CiteScore
1.30
自引率
0.00%
发文量
49
期刊介绍: The aim of the Russian-language journal "Biomeditsinskaya Khimiya" (Biomedical Chemistry) is to introduce the latest results obtained by scientists from Russia and other Republics of the Former Soviet Union. The Journal will cover all major areas of Biomedical chemistry, including neurochemistry, clinical chemistry, molecular biology of pathological processes, gene therapy, development of new drugs and their biochemical pharmacology, introduction and advertisement of new (biochemical) methods into experimental and clinical medicine etc. The Journal also publish review articles. All issues of journal usually contain invited reviews. Papers written in Russian contain abstract (in English).
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