The impact of stress on the behavior of C57BL/6 mice with liver injury: a comparative study

IF 2.6 3区医学 Q2 BEHAVIORAL SCIENCES

Frontiers in Behavioral Neuroscience Pub Date : 2024-02-26 DOI:10.3389/fnbeh.2024.1358964

Mădălina Iuliana Mușat, Smaranda Ioana Mitran, Ion Udriștoiu, Carmen Valeria Albu, Bogdan Cătălin

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引用次数: 0

Abstract

Introduction

Depressive-like behavior has been shown to be associated with liver damage. This study aimed to evaluate the impact of three different models of depression on the behavior of mice with liver injury.

Methods

During the 4 weeks of methionine/choline deficiency diet (MCD), adult C57BL/6 mice were randomly divided into four groups: MCD (no stress protocol, n = 6), chronic unpredictable mild stress (CUMS, n = 9), acute and repeated forced swim stress [aFSS (n = 9) and rFSS (n = 9)].

Results

All depression protocols induced increased anhedonia and anxiety-like behavior compared to baseline and had no impact on the severity of liver damage, according to ultrasonography. However, different protocols evoked different overall behavior patterns. After the depressive-like behavior induction protocols, animals subjected to aFSS did not exhibit anxiety-like behavior differences compared to MCD animals, while mice subjected to CUMS showed additional weight loss compared to FSS animals. All tested protocols for inducing depressive-like behavior decreased the short-term memory of mice with liver damage, as assessed by the novel object recognition test (NORT).

Discussion

Our results show that the use of all protocols seems to generate different levels of anxiety-like behavior, but only the depressive-like behavior induction procedures associate additional anhedonia and memory impairment in mice with liver injury.

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压力对肝损伤 C57BL/6 小鼠行为的影响：一项比较研究

简介：抑郁样行为已被证明与肝损伤有关。本研究旨在评估三种不同抑郁模型对肝损伤小鼠行为的影响。方法在蛋氨酸/胆碱缺乏饮食（MCD）4周期间，将成年C57BL/6小鼠随机分为四组：结果与基线相比，所有抑郁方案都会诱发更多的失乐症和焦虑样行为，而且根据超声波检查，这些方案对肝损伤的严重程度没有影响。然而，不同的方案会诱发不同的整体行为模式。在抑郁样行为诱导方案后，与MCD动物相比，接受aFSS诱导的动物没有表现出焦虑样行为差异，而与FSS诱导的动物相比，接受CUMS诱导的小鼠表现出额外的体重减轻。讨论我们的研究结果表明，使用所有诱导抑郁样行为的方案似乎都会产生不同程度的焦虑样行为，但只有抑郁样行为诱导程序会导致肝损伤小鼠出现额外的失神和记忆损伤。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Frontiers in Behavioral Neuroscience BEHAVIORAL SCIENCES-NEUROSCIENCES

CiteScore

4.70

自引率

3.30%

发文量

506

审稿时长

6-12 weeks

期刊介绍： Frontiers in Behavioral Neuroscience is a leading journal in its field, publishing rigorously peer-reviewed research that advances our understanding of the neural mechanisms underlying behavior. Field Chief Editor Nuno Sousa at the Instituto de Pesquisa em Ciências da Vida e da Saúde (ICVS) is supported by an outstanding Editorial Board of international experts. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to researchers, academics, clinicians and the public worldwide. This journal publishes major insights into the neural mechanisms of animal and human behavior, and welcomes articles studying the interplay between behavior and its neurobiological basis at all levels: from molecular biology and genetics, to morphological, biochemical, neurochemical, electrophysiological, neuroendocrine, pharmacological, and neuroimaging studies.