Let it stick: Strategies and applications for intracellular plasma membrane targeting of proteins in Saccharomyces cerevisiae.

IF 2.2 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Yeast Pub Date : 2024-05-01 Epub Date: 2024-03-05 DOI:10.1002/yea.3933
Liv Teresa Muth, Inge Noëlle Adriënne Van Bogaert
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引用次数: 0

Abstract

Lipid binding domains and protein lipidations are essential features to recruit proteins to intracellular membranes, enabling them to function at specific sites within the cell. Membrane association can also be exploited to answer fundamental and applied research questions, from obtaining insights into the understanding of lipid metabolism to employing them for metabolic engineering to redirect fluxes. This review presents a broad catalog of membrane binding strategies focusing on the plasma membrane of Saccharomyces cerevisiae. Both lipid binding domains (pleckstrin homology, discoidin-type C2, kinase associated-1, basic-rich and bacterial phosphoinositide-binding domains) and co- and post-translational lipidations (prenylation, myristoylation and palmitoylation) are introduced as tools to target the plasma membrane. To provide a toolset of membrane targeting modules, respective candidates that facilitate plasma membrane targeting are showcased including their in vitro and in vivo properties. The relevance and versatility of plasma membrane targeting modules are further highlighted by presenting a selected set of use cases.

Abstract Image

让它粘住:酿酒酵母细胞内质膜靶向蛋白质的策略和应用。
脂质结合域和蛋白质脂质化是将蛋白质吸附到细胞内膜上的基本特征,使它们能够在细胞内的特定部位发挥作用。膜结合还可用于回答基础研究和应用研究问题,从深入了解脂质代谢,到将其用于代谢工程以重定向通量。本综述以酿酒酵母的质膜为重点,介绍了广泛的膜结合策略。其中介绍了脂质结合结构域(pleckstrin homology、discoidin-type C2、激酶相关-1、富碱性和细菌磷脂结合结构域)以及共翻译和翻译后脂质化(前酰化、肉豆蔻酰化和棕榈酰化),作为靶向质膜的工具。为了提供一个膜靶向模块工具集,本文展示了促进质膜靶向的各种候选模块,包括它们的体外和体内特性。通过介绍一组精选的使用案例,进一步强调了质膜靶向模块的相关性和多功能性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Yeast
Yeast 生物-生化与分子生物学
CiteScore
5.30
自引率
3.80%
发文量
55
审稿时长
3 months
期刊介绍: Yeast publishes original articles and reviews on the most significant developments of research with unicellular fungi, including innovative methods of broad applicability. It is essential reading for those wishing to keep up to date with this rapidly moving field of yeast biology. Topics covered include: biochemistry and molecular biology; biodiversity and taxonomy; biotechnology; cell and developmental biology; ecology and evolution; genetics and genomics; metabolism and physiology; pathobiology; synthetic and systems biology; tools and resources
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