Antimicrobial and antitumor activities of an alginate-based membrane loaded with bismuth nanoparticles and cetylpyridinium chloride.

IF 3.1 4区医学 Q2 BIOPHYSICS

Journal of Applied Biomaterials & Functional Materials Pub Date : 2024-01-01 DOI:10.1177/22808000241236590

Claudio Cabral-Romero, Rene Hernández-Delgadillo, Sergio Eduardo Nakagoshi-Cepeda, Rosa Isela Sánchez-Najéra, Erandi Escamilla-García, Juan Manuel Solís-Soto, Claudia María García-Cuellar, Yesennia Sánchez-Pérez, Samantha Maribel Flores-Treviño, Nayely Pineda-Aguilar, Juan Valerio Cauich-Rodríguez, Irene Meester, Shankararaman Chellam

{"title":"Antimicrobial and antitumor activities of an alginate-based membrane loaded with bismuth nanoparticles and cetylpyridinium chloride.","authors":"Claudio Cabral-Romero, Rene Hernández-Delgadillo, Sergio Eduardo Nakagoshi-Cepeda, Rosa Isela Sánchez-Najéra, Erandi Escamilla-García, Juan Manuel Solís-Soto, Claudia María García-Cuellar, Yesennia Sánchez-Pérez, Samantha Maribel Flores-Treviño, Nayely Pineda-Aguilar, Juan Valerio Cauich-Rodríguez, Irene Meester, Shankararaman Chellam","doi":"10.1177/22808000241236590","DOIUrl":null,"url":null,"abstract":"Objective: To evaluate the antitumor and antimicrobial properties of an alginate-based membrane (ABM) loaded with bismuth lipophilic nanoparticles (BisBAL NPs) and cetylpyridinium chloride (CPC) on clinically isolated bacteria and a pancreatic cancer cell line.Material and methods: The BisBAL NP-CPC ABM was characterized using optical and scanning electron microscopy (SEM). The antimicrobial potential was measured using the disk-diffusion assay, and antibiofilm activity was determined through the live/dead assay and fluorescence microscopy. The antitumor activity was analyzed on the pancreatic cell line (Panc 03.27) using the MTT assay and live/dead assay with fluorescence microscopy.Results: After a 24-h exposure (37°C, aerobic conditions), 5 µM BisBAL NP reduced the growth of K. pneumoniae by 77.9%, while 2.5 µM BisBAL NP inhibited the growth of Salmonella, E. faecalis and E. faecium by 82.9%, 82.6%, and 78%, respectively (p < 0.0001). The BisBAL NPs-CPC ABM (at a ratio of 10:1; 500 and 50 µM, respectively) inhibited the growth of all isolated bacteria, producing inhibition halos of 9.5, 11.2, 7, and 10.3 mm for K. pneumoniae, Salmonella, E. faecalis, and E. faecium, respectively, in contrast to the 6.5, 9.5, 8.5, and 9.8 mm obtained with 100 µM ceftriaxone (p < 0.0001). The BisBAL NPs-CPC ABM also reduced bacterial biofilms, with 81.4%, 74.5%, 97.1%, and 79.5% inhibition for K. pneumoniae, E. faecium, E. faecalis, and Salmonella, respectively. Furthermore, the BisBAL NPs-CPC ABM decreased Panc 03.27 cell growth by 76%, compared to 18% for drug-free ABM. GEM-ABM reduced tumoral growth by 73%. The live/dead assay confirmed that BisBAL NPs-CPC-ABM and GEM-ABM were cytotoxic for the turmoral Panc 03.27 cells.Conclusion: An alginate-based membrane loaded with BisBAL NP and CPC exhibits dual antimicrobial and antitumoral efficacy. Therefore, it could be applied in cancer treatment and to diminish the occurrence of surgical site infections.","PeriodicalId":14985,"journal":{"name":"Journal of Applied Biomaterials & Functional Materials","volume":null,"pages":null},"PeriodicalIF":3.1000,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Applied Biomaterials & Functional Materials","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1177/22808000241236590","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"BIOPHYSICS","Score":null,"Total":0}

引用次数: 0

Abstract

Objective: To evaluate the antitumor and antimicrobial properties of an alginate-based membrane (ABM) loaded with bismuth lipophilic nanoparticles (BisBAL NPs) and cetylpyridinium chloride (CPC) on clinically isolated bacteria and a pancreatic cancer cell line.

Material and methods: The BisBAL NP-CPC ABM was characterized using optical and scanning electron microscopy (SEM). The antimicrobial potential was measured using the disk-diffusion assay, and antibiofilm activity was determined through the live/dead assay and fluorescence microscopy. The antitumor activity was analyzed on the pancreatic cell line (Panc 03.27) using the MTT assay and live/dead assay with fluorescence microscopy.

Results: After a 24-h exposure (37°C, aerobic conditions), 5 µM BisBAL NP reduced the growth of K. pneumoniae by 77.9%, while 2.5 µM BisBAL NP inhibited the growth of Salmonella, E. faecalis and E. faecium by 82.9%, 82.6%, and 78%, respectively (p < 0.0001). The BisBAL NPs-CPC ABM (at a ratio of 10:1; 500 and 50 µM, respectively) inhibited the growth of all isolated bacteria, producing inhibition halos of 9.5, 11.2, 7, and 10.3 mm for K. pneumoniae, Salmonella, E. faecalis, and E. faecium, respectively, in contrast to the 6.5, 9.5, 8.5, and 9.8 mm obtained with 100 µM ceftriaxone (p < 0.0001). The BisBAL NPs-CPC ABM also reduced bacterial biofilms, with 81.4%, 74.5%, 97.1%, and 79.5% inhibition for K. pneumoniae, E. faecium, E. faecalis, and Salmonella, respectively. Furthermore, the BisBAL NPs-CPC ABM decreased Panc 03.27 cell growth by 76%, compared to 18% for drug-free ABM. GEM-ABM reduced tumoral growth by 73%. The live/dead assay confirmed that BisBAL NPs-CPC-ABM and GEM-ABM were cytotoxic for the turmoral Panc 03.27 cells.

Conclusion: An alginate-based membrane loaded with BisBAL NP and CPC exhibits dual antimicrobial and antitumoral efficacy. Therefore, it could be applied in cancer treatment and to diminish the occurrence of surgical site infections.

查看原文本刊更多论文

负载纳米铋颗粒和氯化十六烷基吡啶的藻酸盐膜的抗菌和抗肿瘤活性。

目的评估负载有亲脂性纳米粒子铋（BisBAL NPs）和氯化十六烷基吡啶（CPC）的藻酸盐基膜（ABM）对临床分离细菌和胰腺癌细胞系的抗肿瘤和抗菌特性：使用光学和扫描电子显微镜（SEM）对 BisBAL NP-CPC ABM 进行了表征。使用盘扩散试验测量了抗菌潜力，并通过活/死试验和荧光显微镜确定了抗生物膜活性。在胰腺细胞系（Panc 03.27）上使用 MTT 试验和荧光显微镜进行活/死试验，分析其抗肿瘤活性：暴露 24 小时后（37°C，有氧条件下），5 µM BisBAL NP 可使肺炎双球菌的生长速度降低 77.9%，而 2.5 µM BisBAL NP 可使沙门氏菌、粪大肠杆菌和粪肠球菌的生长速度分别降低 82.9%、82.6% 和 78% (p)。BisBAL NPs-CPC ABM（比例为 10:1；分别为 500 µM 和 50 µM）可抑制所有分离细菌的生长，对肺炎双球菌、沙门氏菌、粪大肠杆菌和粪肠球菌分别产生 9.5、11.2、7 和 10.3 毫米的抑制光晕，而 100 µM 头孢曲松的抑制光晕分别为 6.5、9.5、8.5 和 9.8 毫米（p ）。BisBAL NPs-CPC ABM 还能减少细菌生物膜，对肺炎双球菌、粪大肠杆菌、屎大肠杆菌和沙门氏菌的抑制率分别为 81.4%、74.5%、97.1% 和 79.5%。此外，BisBAL NPs-CPC ABM 可使 Panc 03.27 细胞的生长速度降低 76%，而不含药物的 ABM 只降低了 18%。GEM-ABM 可使肿瘤生长减少 73%。活/死试验证实，BisBAL NPs-CPC-ABM 和 GEM-ABM 对胰腺 03.27 细胞具有细胞毒性：结论：负载有 BisBAL NP 和 CPC 的藻酸盐膜具有抗菌和抗肿瘤双重功效。因此，它可用于癌症治疗和减少手术部位感染的发生。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of Applied Biomaterials & Functional Materials BIOPHYSICS-ENGINEERING, BIOMEDICAL

CiteScore

4.40

自引率

4.00%

发文量

审稿时长

>12 weeks

期刊介绍： The Journal of Applied Biomaterials & Functional Materials (JABFM) is an open access, peer-reviewed, international journal considering the publication of original contributions, reviews and editorials dealing with clinical and laboratory investigations in the fast growing field of biomaterial sciences and functional materials. The areas covered by the journal will include: • Biomaterials / Materials for biomedical applications • Functional materials • Hybrid and composite materials • Soft materials • Hydrogels • Nanomaterials • Gene delivery • Nonodevices • Metamaterials • Active coatings • Surface functionalization • Tissue engineering • Cell delivery/cell encapsulation systems • 3D printing materials • Material characterization • Biomechanics