CmbHLH110, a novel bHLH transcription factor, accelerates flowering in chrysanthemum

IF 5.7 1区农林科学 Q1 HORTICULTURE

Horticultural Plant Journal Pub Date : 2024-02-28 DOI:10.1016/j.hpj.2023.05.022

Yaoyao Huang, Xiaojuan Xing, Jinyu Jin, Yun Tang, Lian Ding, Aiping Song, Sumei Chen, Fadi Chen, Jiafu Jiang, Weimin Fang

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引用次数: 0

Abstract

Basic helix–loop–helix (bHLH) transcription factor gene family in plants controls various growth and development aspects; however, the actual roles of these genes in flowering plants are not well known. In this study, a novel bHLH protein CmbHLH110 was found to interact with CmERF110 by and experiments, a chrysanthemum ERF110 homolog that acts as a positive flowering regulator. In addition, was also found to regulate the flowering of chrysanthemums, overexpression of causes chrysanthemums to flower earlier, and suppressed leads to delayed flowering. Furthermore, the loss-of-function mutant of its homologue () had a noticeable late flowering phenotype, and completely complemented the late flowering phenotype of the mutant, whereas heterologous overexpression of in Col-0 caused early flowering. Transcriptome sequencing revealed significant differential expression of flowering-related and circadian clock-related genes in transgenic chrysanthemum. Therefore, we concluded that CmbHLH110, as a novel flowering regulator, could interact with CmERF110 to regulate flowering in chrysanthemum.

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新型 bHLH 转录因子 CmbHLH110 可加速菊花开花

植物中的碱性螺旋-环-螺旋（bHLH）转录因子基因家族控制着植物生长发育的各个方面；然而，这些基因在开花植物中的实际作用并不十分清楚。本研究通过和实验发现了一种新型 bHLH 蛋白 CmbHLH110 与 CmERF110 相互作用，CmERF110 是菊花 ERF110 的同源物，具有积极的开花调节作用。此外，研究还发现，mbHLH110 还能调节菊花的开花，过量表达会导致菊花提前开花，而抑制则会导致菊花延迟开花。此外，其同源物（）的功能缺失突变体具有明显的晚花表型，并完全补充了突变体的晚花表型，而在 Col-0 中异源过表达则会导致早花。转录组测序显示，转基因菊花中开花相关基因和昼夜节律相关基因的表达存在显著差异。因此，我们认为 CmbHLH110 作为一种新型的开花调节因子，可以与 CmERF110 相互作用来调节菊花的开花。

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来源期刊

Horticultural Plant Journal Environmental Science-Ecology

CiteScore

9.60

自引率

14.00%

发文量

293

审稿时长

33 weeks

期刊介绍： Horticultural Plant Journal (HPJ) is an OPEN ACCESS international journal. HPJ publishes research related to all horticultural plants, including fruits, vegetables, ornamental plants, tea plants, and medicinal plants, etc. The journal covers all aspects of horticultural crop sciences, including germplasm resources, genetics and breeding, tillage and cultivation, physiology and biochemistry, ecology, genomics, biotechnology, plant protection, postharvest processing, etc. Article types include Original research papers, Reviews, and Short communications.