Using the traditional microscope for mineral grain orientation determination: A prototype image analysis pipeline for optic-axis mapping (POAM)

IF 1.5 4区 工程技术 Q3 MICROSCOPY
Marco Andres Acevedo Zamora, Christoph Eckart Schrank, Balz Samuel Kamber
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引用次数: 0

Abstract

This paper reports on the development of an open-source image analysis software ‘pipeline’ dedicated to petrographic microscopy. Using conventional rock thin sections and images from a standard polarising microscope, the pipeline can classify minerals and subgrains into objects and obtain information about optic-axis orientation. Five metamorphic rocks were chosen to test and illustrate the method. Thin sections were imaged using reflected and cross- and plane-polarised transmitted light. Images were taken at different angles of the polariser and analyser (360° with 10° steps), both with and without the full-lambda plate. The resulting image stacks were analysed with a modular pipeline for optic-axis mapping (POAM). POAM consists of external and internal software packages that register, segment, classify, and interpret the visible light spectra using object-based image analysis (OBIAS). The mapped fields-of-view and grain orientation stereonets of interest are presented in the context of whole-slide images.

Two innovations are reported. First, we used hierarchical tree region merging on blended multimodal images to classify individual grains of rock-forming minerals into objects. Second, we assembled a new optical mineralogy algorithm chain that identifies the mineral slow axis orientation. The c-axis orientation results were verified with scanning electron microscopy electron backscattered diffraction (SEM-EBSD) data. For quartz (uniaxial) in a granite mylonite the test yielded excellent correspondence of c-axis azimuth and good agreement for inclination. For orthorhombic orthopyroxene in a deformed garnet harzburgite, POAM produced acceptable results for slow axis azimuth. In addition, the method identified slight anisotropy in garnet that would not be appreciated by traditional microscopy.

We propose that our method is ideally suited for two commonly performed tasks in mineralogy. First, for mineral grain classification of entire thin sections scans on blended images to provide automated modal abundance estimates and grain size distribution. Second, for prospective fields of view of interest, POAM can rapidly generate slow axis crystal orientation maps from multiangle image stacks on conventionally prepared thin sections for targeting detailed SEM-EBSD studies.

Abstract Image

使用传统显微镜确定矿物晶粒取向:用于光轴绘图(POAM)的原型图像分析管道。
本文介绍了一种专用于岩石学显微镜的开源图像分析软件 "管道 "的开发情况。利用传统的岩石薄片和标准偏光显微镜的图像,该管道可将矿物和亚晶粒分类为对象,并获取有关光轴方向的信息。我们选择了五种变质岩来测试和说明该方法。使用反射光、交叉偏振光和平面偏振透射光对薄片进行成像。在偏振镜和分析仪的不同角度(360°,每步 10°),使用或不使用全λ板进行成像。通过模块化的光轴映射管道(POAM)对得到的图像堆栈进行分析。POAM 由外部和内部软件包组成,使用基于对象的图像分析(OBIAS)对可见光光谱进行注册、分割、分类和解释。绘制的视场和谷物取向立体图在整张幻灯片图像中呈现。报告有两项创新。首先,我们在混合多模态图像上使用了分层树区域合并技术,将成岩矿物的单个晶粒归类为对象。其次,我们组建了一个新的光学矿物学算法链,可识别矿物慢轴方向。c 轴取向结果通过扫描电子显微镜电子反向散射衍射(SEM-EBSD)数据进行了验证。对于花岗岩麦饭石中的石英(单轴),测试得出了极好的 c 轴方位角对应关系和良好的倾斜度对应关系。对于变形石榴石哈兹堡岩中的正长方正长石,POAM 得出的慢轴方位角结果是可以接受的。此外,该方法还发现了石榴石中的轻微各向异性,而传统的显微镜检查是无法发现这些各向异性的。我们认为,我们的方法非常适合矿物学中的两项常见任务。首先,在混合图像上对整个薄片扫描进行矿物晶粒分类,以提供自动模式丰度估计和晶粒大小分布。其次,对于感兴趣的前瞻性视场,POAM 可以从传统制备的薄片上的多角度图像堆栈中快速生成慢轴晶体取向图,以便进行详细的 SEM-EBSD 研究。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of microscopy
Journal of microscopy 工程技术-显微镜技术
CiteScore
4.30
自引率
5.00%
发文量
83
审稿时长
1 months
期刊介绍: The Journal of Microscopy is the oldest journal dedicated to the science of microscopy and the only peer-reviewed publication of the Royal Microscopical Society. It publishes papers that report on the very latest developments in microscopy such as advances in microscopy techniques or novel areas of application. The Journal does not seek to publish routine applications of microscopy or specimen preparation even though the submission may otherwise have a high scientific merit. The scope covers research in the physical and biological sciences and covers imaging methods using light, electrons, X-rays and other radiations as well as atomic force and near field techniques. Interdisciplinary research is welcome. Papers pertaining to microscopy are also welcomed on optical theory, spectroscopy, novel specimen preparation and manipulation methods and image recording, processing and analysis including dynamic analysis of living specimens. Publication types include full papers, hot topic fast tracked communications and review articles. Authors considering submitting a review article should contact the editorial office first.
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