Zein nanoparticles containing ceftazidime and tobramycin: antibacterial activity against Gram-negative bacteria.

IF 2.5 4区 生物学 Q3 MICROBIOLOGY
Future microbiology Pub Date : 2024-03-01 Epub Date: 2024-03-05 DOI:10.2217/fmb-2023-0147
Luís Aa Campos, Azael Fs Neto, Maria Cs Noronha, João Vo Santos, Marton Ka Cavalcante, Maria Cab Castro, Valéria Ra Pereira, Isabella Mf Cavalcanti, Nereide S Santos-Magalhães
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引用次数: 0

Abstract

Aims: This work describes the encapsulation of ceftazidime and tobramycin in zein nanoparticles (ZNPs) and the characterization of their antibacterial and antibiofilm activities against Gram-negative bacteria. Materials & methods: ZNPs were synthesized by nanoprecipitation. Cytotoxicity was assessed by MTT assay and antibacterial and antibiofilm assays were performed by broth microdilution and violet crystal techniques. Results: ZNPs containing ceftazidime (CAZ-ZNPs) and tobramycin (TOB-ZNPs) showed drug encapsulation and thermal stability. Encapsulation of the drugs reduced their cytotoxicity 9-25-fold. Antibacterial activity, inhibition and eradication of biofilm by CAZ-ZNPs and TOB-ZNPs were observed. There was potentiation when CAZ-ZNPs and TOB-ZNPs were combined. Conclusion: CAZ-ZNPs and TOB-ZNPs present ideal physical characteristics for in vivo studies of antibacterial and antibiofilm activities.

含有头孢他啶和妥布霉素的 Zein 纳米粒子:对革兰氏阴性菌的抗菌活性。
目的:本研究介绍了头孢他啶和妥布霉素在沸石纳米颗粒(ZNPs)中的封装及其对革兰氏阴性菌的抗菌和抗生物膜活性。材料与方法:采用纳米沉淀法合成 ZNPs。细胞毒性通过 MTT 试验进行评估,抗菌和抗生物膜试验通过肉汤微量稀释和紫晶体技术进行。结果含有头孢他啶(CAZ-ZNPs)和妥布霉素(TOB-ZNPs)的 ZNPs 具有药物包封性和热稳定性。药物的封装使其细胞毒性降低了 9-25 倍。观察到 CAZ-ZNPs 和 TOB-ZNPs 具有抗菌活性,能抑制和消除生物膜。当 CAZ-ZNPs 和 TOB-ZNPs 结合使用时,抗菌效果更强。结论CAZ-ZNPs 和 TOB-ZNPs 为体内抗菌和抗生物膜活性研究提供了理想的物理特性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Future microbiology
Future microbiology 生物-微生物学
CiteScore
4.90
自引率
3.20%
发文量
134
审稿时长
6-12 weeks
期刊介绍: Future Microbiology delivers essential information in concise, at-a-glance article formats. Key advances in the field are reported and analyzed by international experts, providing an authoritative but accessible forum for this increasingly important and vast area of research.
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