Zein nanoparticles containing ceftazidime and tobramycin: antibacterial activity against Gram-negative bacteria.

IF 2.5 4区生物学 Q3 MICROBIOLOGY

Future microbiology Pub Date : 2024-03-01 Epub Date: 2024-03-05 DOI:10.2217/fmb-2023-0147

Luís Aa Campos, Azael Fs Neto, Maria Cs Noronha, João Vo Santos, Marton Ka Cavalcante, Maria Cab Castro, Valéria Ra Pereira, Isabella Mf Cavalcanti, Nereide S Santos-Magalhães

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引用次数: 0

Abstract

Aims: This work describes the encapsulation of ceftazidime and tobramycin in zein nanoparticles (ZNPs) and the characterization of their antibacterial and antibiofilm activities against Gram-negative bacteria. Materials & methods: ZNPs were synthesized by nanoprecipitation. Cytotoxicity was assessed by MTT assay and antibacterial and antibiofilm assays were performed by broth microdilution and violet crystal techniques. Results: ZNPs containing ceftazidime (CAZ-ZNPs) and tobramycin (TOB-ZNPs) showed drug encapsulation and thermal stability. Encapsulation of the drugs reduced their cytotoxicity 9-25-fold. Antibacterial activity, inhibition and eradication of biofilm by CAZ-ZNPs and TOB-ZNPs were observed. There was potentiation when CAZ-ZNPs and TOB-ZNPs were combined. Conclusion: CAZ-ZNPs and TOB-ZNPs present ideal physical characteristics for in vivo studies of antibacterial and antibiofilm activities.

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含有头孢他啶和妥布霉素的 Zein 纳米粒子：对革兰氏阴性菌的抗菌活性。

目的：本研究介绍了头孢他啶和妥布霉素在沸石纳米颗粒（ZNPs）中的封装及其对革兰氏阴性菌的抗菌和抗生物膜活性。材料与方法：采用纳米沉淀法合成 ZNPs。细胞毒性通过 MTT 试验进行评估，抗菌和抗生物膜试验通过肉汤微量稀释和紫晶体技术进行。结果含有头孢他啶（CAZ-ZNPs）和妥布霉素（TOB-ZNPs）的 ZNPs 具有药物包封性和热稳定性。药物的封装使其细胞毒性降低了 9-25 倍。观察到 CAZ-ZNPs 和 TOB-ZNPs 具有抗菌活性，能抑制和消除生物膜。当 CAZ-ZNPs 和 TOB-ZNPs 结合使用时，抗菌效果更强。结论CAZ-ZNPs 和 TOB-ZNPs 为体内抗菌和抗生物膜活性研究提供了理想的物理特性。

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来源期刊

Future microbiology 生物-微生物学

CiteScore

4.90

自引率

3.20%

发文量

134

审稿时长

6-12 weeks

期刊介绍： Future Microbiology delivers essential information in concise, at-a-glance article formats. Key advances in the field are reported and analyzed by international experts, providing an authoritative but accessible forum for this increasingly important and vast area of research.