Genetic dissection and identification of stripe rust resistance genes in the wheat cultivar Lanhangxuan 121, a cultivar selected from a space mutation population

IF 2.6 3区农林科学 Q1 AGRONOMY

Molecular Breeding Pub Date : 2024-03-04 DOI:10.1007/s11032-024-01461-0

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引用次数: 0

Abstract

Stripe rust is a devastating disease of wheat worldwide. Chinese wheat cultivar Lanhangxuan 121 (LHX121), selected from an advanced line L92-47 population that had been subjected to space mutation breeding displayed a consistently higher level of resistance to stipe rust than its parent in multiple field environments. The aim of this research was to establish the number and types of resistance genes in parental lines L92-47 and LHX121 using separate segregating populations. The first population developed from a cross between LHX121 and susceptible cultivar Xinong 822 comprised 278 F_2:3 lines. The second validation population comprised 301 F_2:3 lines from a cross between L92-47 and susceptible cultivar Xinong 979. Lines of two population were evaluated for stripe rust response at three sites during the 2018–2020 cropping season. Affymetrix 660 K SNP arrays were used to genotype the lines and parents. Inclusive composite interval mapping detected QTL QYrLHX.nwafu-2BS, QYrLHX.nwafu-3BS, and QYrLHX.nwafu-5BS for resistance in all three environments. Based on previous studies and pedigree information, QYrLHX.nwafu-2BS and QYrLHX.nwafu-3BS were likely to be Yr27 and Yr30 that are present in the L92-47 parent. QYrLHX.nwafu-5BS (YrL121) detected only in LHX121 was mapped to a 7.60 cM interval and explained 10.67–22.57% of the phenotypic variation. Compared to stripe rust resistance genes previously mapped to chromosome 5B, YrL121 might be a new adult plant resistance QTL. Furthermore, there were a number of variations signals using 35 K SNP array and differentially expressed genes using RNA-seq between L92-47 and LHX121 in the YrL121 region, indicating that they probably impair the presence and/or function of YrL121.

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从空间突变群体中选育的小麦栽培品种兰航轩 121 的抗条锈病基因的遗传分析和鉴定

摘要条锈病是世界范围内小麦的一种毁灭性病害。从经过空间突变育种的先进品系 L92-47 群体中选育出的中国小麦栽培品种兰航轩 121（LHX121）在多种田间环境中表现出持续高于其亲本的抗条锈病能力。这项研究的目的是利用不同的分离群体，确定亲本品系 L92-47 和 LHX121 中抗性基因的数量和类型。第一个群体由 LHX121 和易感性栽培品种新农 822 杂交而成，包括 278 个 F2:3 株系。第二个验证群体由 301 个 F2:3 株系组成，这些株系来自 L92-47 与易感性栽培品种新农 979 的杂交。2018-2020 年种植季期间，在三个地点对两个群体的品系进行了条锈病反应评估。Affymetrix 660 K SNP 阵列用于对品系和亲本进行基因分型。包容性复合间隔图谱在所有三种环境中都检测到了抗性QTL QYrLHX.nwafu-2BS、QYrLHX.nwafu-3BS和QYrLHX.nwafu-5BS。根据先前的研究和血统信息，QYrLHX.nwafu-2BS 和 QYrLHX.nwafu-3BS 很可能是存在于 L92-47 亲本中的 Yr27 和 Yr30。仅在 LHX121 中检测到的 QYrLHX.nwafu-5BS（YrL121）被映射到 7.60 cM 区间，解释了 10.67-22.57% 的表型变异。与之前绘制在 5B 染色体上的条锈病抗性基因相比，YrL121 可能是一个新的成株抗性 QTL。此外，在 YrL121 区域，利用 35 K SNP 阵列和 RNA-seq 分析发现 L92-47 与 LHX121 之间存在一些变异信号和差异表达基因，这表明它们可能会影响 YrL121 的存在和/或功能。

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来源期刊

Molecular Breeding 农林科学-农艺学

CiteScore

5.60

自引率

6.50%

发文量

审稿时长

1.5 months

期刊介绍： Molecular Breeding is an international journal publishing papers on applications of plant molecular biology, i.e., research most likely leading to practical applications. The practical applications might relate to the Developing as well as the industrialised World and have demonstrable benefits for the seed industry, farmers, processing industry, the environment and the consumer. All papers published should contribute to the understanding and progress of modern plant breeding, encompassing the scientific disciplines of molecular biology, biochemistry, genetics, physiology, pathology, plant breeding, and ecology among others. Molecular Breeding welcomes the following categories of papers: full papers, short communications, papers describing novel methods and review papers. All submission will be subject to peer review ensuring the highest possible scientific quality standards. Molecular Breeding core areas: Molecular Breeding will consider manuscripts describing contemporary methods of molecular genetics and genomic analysis, structural and functional genomics in crops, proteomics and metabolic profiling, abiotic stress and field evaluation of transgenic crops containing particular traits. Manuscripts on marker assisted breeding are also of major interest, in particular novel approaches and new results of marker assisted breeding, QTL cloning, integration of conventional and marker assisted breeding, and QTL studies in crop plants.