MicroRNA-34b-5p increases chemosensitivity of hepatocellular carcinoma cells

IF 1.1 4区医学 Q4 TOXICOLOGY

Molecular & Cellular Toxicology Pub Date : 2024-03-04 DOI:10.1007/s13273-024-00431-z

JinSuo Chen, LiNa Wang, XueMei Wu, ZhiJie Ding, WenXi Cao, LeiLei Yang, YongPing Zhou, Li Xia, Zhao Song

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Abstract

Objective

Increasing evidence shows that microRNA (miR) is related to drug resistance in hepatocellular carcinoma (HCC). miR-34b-5p could retard tumor development, but its function in HCC is still unclear.

Methods

miR-34b-5p expression was determined in HCC tissues and cell lines. Analysis and verification of the binding between miR-34b-5p and metadherin (MTDH) was carried out. The actual action of miR-34b-5p and MTDH in the area of proliferation, apoptosis, invasion, and migration of cisplatin (DDP)-resistant HCC cells was monitored.

Results

miR-34b-5p was downregulated in HCC. Overexpression of miR-34b-5p enhanced the sensitivity of HCC cells to DDP, inhibiting proliferation, migration, and invasion and promoting apoptosis. MTDH was the direct target of miR-34b-5p. MTDH was upregulated in HCC tissues, which was negatively correlated with miR-34b-5p expression. Enhancement of MTDH can reverse the effect of upregulated miR-34b-5p on the chemosensitivity of HCC cells.

Conclusion

miR-34b-5p targets MTDH and enhances the chemosensitivity of HCC cells.

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MicroRNA-34b-5p 可提高肝癌细胞的化疗敏感性

目的越来越多的证据表明，微RNA（miR）与肝细胞癌（HCC）的耐药性有关。miR-34b-5p可延缓肿瘤的发展，但其在HCC中的功能尚不清楚。分析并验证了 miR-34b-5p 与偏球蛋白（MTDH）的结合。结果miR-34b-5p在HCC中下调。过表达 miR-34b-5p 能增强 HCC 细胞对 DDP 的敏感性，抑制细胞增殖、迁移和侵袭，促进细胞凋亡。MTDH是miR-34b-5p的直接靶标。MTDH在HCC组织中上调，与miR-34b-5p的表达呈负相关。结论miR-34b-5p以MTDH为靶点，增强了HCC细胞的化疗敏感性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecular & Cellular Toxicology 医学-毒理学

CiteScore

2.50

自引率

17.60%

发文量

114

审稿时长

6-12 weeks

期刊介绍： Molecular & Cellular Toxicology publishes original research and reviews in all areas of the complex interaction between the cell´s genome (the sum of all genes within the chromosome), chemicals in the environment, and disease. Acceptable manuscripts are the ones that deal with some topics of environmental contaminants, including those that lie in the domains of analytical chemistry, biochemistry, pharmacology and toxicology with the aspects of molecular and cellular levels. Emphasis will be placed on toxic effects observed at relevant genomics and proteomics, which have direct impact on drug development, environment health, food safety, preventive medicine, and forensic medicine. The journal is committed to rapid peer review to ensure the publication of highest quality original research and timely news and review articles.