The A2 haplotype of Echinococcus multilocularis is the predominant variant infecting humans and dogs in Yili Prefecture, Xinjiang

IF 2.6 4区医学 Q3 INFECTIOUS DISEASES

Infection Genetics and Evolution Pub Date : 2024-03-01 DOI:10.1016/j.meegid.2024.105581

Baoping Guo , Cairen , Jianyong Wu , Wenli Wang , Bingjie Wang , Yuan Ren , Mayire Aizezi , Rongsheng Mi , Li Zhao , Hao Wen

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引用次数: 0

Abstract

Alveolar echinococcosis (AE), caused by Echinococcus multilocularis, is an important zoonotic disease. Yili Prefecture in Xinjiang is endemic for AE, however the molecular variability of E. multilocularis in this region is poorly understood. In this study, 127 samples were used for haplotypes analysis, including 79 tissues from humans, 43 liver tissues from small rodents, and 5 fecal samples from dogs. Genetic variability in E. multilocularis was studied using complete sequences of the mitochondrial (mt) genes of cytochrome b (cob), NADH dehydrogenase subunit 2 (nad2), and cytochrome c oxidase subunit 1 (cox1), using a total of 3558 bp per sample. The Asia haplotype 2 (A2) was the dominant haplotype, with 72.15% (57/79) prevalence in humans, 2.33% (1/43) in small rodents, and 80.00% (4/5) in dogs, followed by A5, the second most common haplotype, which infected 27.91% (12/43) small rodents. Haplotype network analysis showed that all haplotypes clustered together with the Asian group. Pairwise fixation index (F_ST) values showed lower level of genetic differentiation between different regions within the country. Compared with the sequences of E. multilocularis from North America and Europe, all concatenated sequences isolated from Yili Prefecture were highly differentiated and formed a single population. The A2 haplotype, analyzed using the cob, nad2, and cox1 genes of E. multilocularis, is the predominant variant in humans and dogs in Yili Prefecture.

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在新疆伊犁州，多形性棘球蚴的 A2 单倍型是感染人和狗的主要变种。

由多形棘球蚴引起的肺泡棘球蚴病（AE）是一种重要的人畜共患疾病。新疆伊犁州是多棘球蚴病的流行区，但人们对该地区多棘球蚴病的分子变异性知之甚少。本研究使用了 127 份样本进行单倍型分析，其中包括 79 份人类组织、43 份小型啮齿动物肝脏组织和 5 份狗的粪便样本。研究使用了细胞色素 b（cob）、NADH 脱氢酶亚基 2（nad2）和细胞色素 c 氧化酶亚基 1（cox1）的线粒体（mt）基因的完整序列，每个样本共使用了 3558 bp。亚洲单倍型 2（A2）是显性单倍型，在人类中的感染率为 72.15%（57/79），在小型啮齿动物中的感染率为 2.33%（1/43），在狗中的感染率为 80.00%（4/5），其次是第二常见的单倍型 A5，在小型啮齿动物中的感染率为 27.91%（12/43）。单倍型网络分析显示，所有单倍型都与亚洲组聚集在一起。配对固定指数（FST）值显示，国内不同地区之间的遗传分化程度较低。与北美和欧洲的多角体E. multilocularis序列相比，伊犁州分离出的所有单倍型序列分化程度较高，形成了一个单一的种群。根据多角体圆线虫的cob、nad2和cox1基因分析，A2单倍型是伊犁州人和狗的主要变种。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Infection Genetics and Evolution 医学-传染病学

CiteScore

8.40

自引率

0.00%

发文量

215

审稿时长

82 days

期刊介绍： (aka Journal of Molecular Epidemiology and Evolutionary Genetics of Infectious Diseases -- MEEGID) Infectious diseases constitute one of the main challenges to medical science in the coming century. The impressive development of molecular megatechnologies and of bioinformatics have greatly increased our knowledge of the evolution, transmission and pathogenicity of infectious diseases. Research has shown that host susceptibility to many infectious diseases has a genetic basis. Furthermore, much is now known on the molecular epidemiology, evolution and virulence of pathogenic agents, as well as their resistance to drugs, vaccines, and antibiotics. Equally, research on the genetics of disease vectors has greatly improved our understanding of their systematics, has increased our capacity to identify target populations for control or intervention, and has provided detailed information on the mechanisms of insecticide resistance. However, the genetics and evolutionary biology of hosts, pathogens and vectors have tended to develop as three separate fields of research. This artificial compartmentalisation is of concern due to our growing appreciation of the strong co-evolutionary interactions among hosts, pathogens and vectors. Infection, Genetics and Evolution and its companion congress [MEEGID](http://www.meegidconference.com/) (for Molecular Epidemiology and Evolutionary Genetics of Infectious Diseases) are the main forum acting for the cross-fertilization between evolutionary science and biomedical research on infectious diseases. Infection, Genetics and Evolution is the only journal that welcomes articles dealing with the genetics and evolutionary biology of hosts, pathogens and vectors, and coevolution processes among them in relation to infection and disease manifestation. All infectious models enter the scope of the journal, including pathogens of humans, animals and plants, either parasites, fungi, bacteria, viruses or prions. The journal welcomes articles dealing with genetics, population genetics, genomics, postgenomics, gene expression, evolutionary biology, population dynamics, mathematical modeling and bioinformatics. We also provide many author benefits, such as free PDFs, a liberal copyright policy, special discounts on Elsevier publications and much more. Please click here for more information on our author services .