Bioinformatic Study of Possible Acute Regulation of Acid Secretion in the Stomach.

IF 2.3 4区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Journal of Membrane Biology Pub Date : 2024-04-01 Epub Date: 2024-03-04 DOI:10.1007/s00232-024-00310-7
Yan Hay Grace Lee, Nicole T Cerf, Nicholas Shalaby, Mónica R Montes, Ronald J Clarke
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引用次数: 0

Abstract

The gastric H+,K+-ATPase is an integral membrane protein which derives energy from the hydrolysis of ATP to transport H+ ions from the parietal cells of the gastric mucosa into the stomach in exchange for K+ ions. It is responsible for the acidic environment of the stomach, which is essential for digestion. Acid secretion is regulated by the recruitment of the H+,K+-ATPase from intracellular stores into the plasma membrane on the ingestion of food. The similar amino acid sequences of the lysine-rich N-termini α-subunits of the H+,K+- and Na+,K+-ATPases, suggests similar acute regulation mechanisms, specifically, an electrostatic switch mechanism involving an interaction of the N-terminal tail with the surface of the surrounding membrane and a modulation of the interaction via regulatory phosphorylation by protein kinases. From a consideration of sequence alignment of the H+,K+-ATPase and an analysis of its coevolution with protein kinase C and kinases of the Src family, the evidence points towards a phosphorylation of tyrosine-7 of the N-terminus by either Lck or Yes in all vertebrates except cartilaginous fish. The results obtained will guide and focus future experimental research.

Abstract Image

胃酸分泌的可能急性调节的生物信息学研究
胃 H+、K+-ATP 酶是一种整体膜蛋白,它通过水解 ATP 获得能量,将 H+ 离子从胃黏膜顶细胞运送到胃中,以交换 K+ 离子。它负责维持胃的酸性环境,这对消化至关重要。摄入食物时,H+,K+-ATP 酶从细胞内储存库招募到质膜,从而调节胃酸分泌。H+,K+-和Na+,K+-ATP酶富含赖氨酸的N-末端α-亚基的氨基酸序列相似,这表明它们具有相似的急性调节机制,特别是涉及N-末端尾部与周围膜表面相互作用的静电转换机制,以及通过蛋白激酶的调节性磷酸化对这种相互作用的调节。通过对 H+,K+-ATPase 的序列比对以及对其与蛋白激酶 C 和 Src 家族激酶共同进化的分析,有证据表明,在除软骨鱼类以外的所有脊椎动物中,N-末端的酪氨酸-7 会被 Lck 或 Yes 磷酸化。获得的结果将指导并聚焦未来的实验研究。
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来源期刊
Journal of Membrane Biology
Journal of Membrane Biology 生物-生化与分子生物学
CiteScore
4.80
自引率
4.20%
发文量
63
审稿时长
6-12 weeks
期刊介绍: The Journal of Membrane Biology is dedicated to publishing high-quality science related to membrane biology, biochemistry and biophysics. In particular, we welcome work that uses modern experimental or computational methods including but not limited to those with microscopy, diffraction, NMR, computer simulations, or biochemistry aimed at membrane associated or membrane embedded proteins or model membrane systems. These methods might be applied to study topics like membrane protein structure and function, membrane mediated or controlled signaling mechanisms, cell-cell communication via gap junctions, the behavior of proteins and lipids based on monolayer or bilayer systems, or genetic and regulatory mechanisms controlling membrane function. Research articles, short communications and reviews are all welcome. We also encourage authors to consider publishing ''negative'' results where experiments or simulations were well performed, but resulted in unusual or unexpected outcomes without obvious explanations. While we welcome connections to clinical studies, submissions that are primarily clinical in nature or that fail to make connections to the basic science issues of membrane structure, chemistry and function, are not appropriate for the journal. In a similar way, studies that are primarily descriptive and narratives of assays in a clinical or population study are best published in other journals. If you are not certain, it is entirely appropriate to write to us to inquire if your study is a good fit for the journal.
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