Multifunctional scaffolds for biomedical applications: Crafting versatile solutions with polycaprolactone enriched by graphene oxide.

IF 6.6 3区 医学 Q1 ENGINEERING, BIOMEDICAL
G Friggeri, I Moretti, F Amato, A G Marrani, F Sciandra, S G Colombarolli, A Vitali, S Viscuso, A Augello, L Cui, G Perini, M De Spirito, M Papi, V Palmieri
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Abstract

The pressing need for multifunctional materials in medical settings encompasses a wide array of scenarios, necessitating specific tissue functionalities. A critical challenge is the occurrence of biofouling, particularly by contamination in surgical environments, a common cause of scaffolds impairment. Beyond the imperative to avoid infections, it is also essential to integrate scaffolds with living cells to allow for tissue regeneration, mediated by cell attachment. Here, we focus on the development of a versatile material for medical applications, driven by the diverse time-definite events after scaffold implantation. We investigate the potential of incorporating graphene oxide (GO) into polycaprolactone (PCL) and create a composite for 3D printing a scaffold with time-controlled antibacterial and anti-adhesive growth properties. Indeed, the as-produced PCL-GO scaffold displays a local hydrophobic effect, which is translated into a limitation of biological entities-attachment, including a diminished adhesion of bacteriophages and a reduction of E. coli and S. aureus adhesion of ∼81% and ∼69%, respectively. Moreover, the ability to 3D print PCL-GO scaffolds with different heights enables control over cell distribution and attachment, a feature that can be also exploited for cellular confinement, i.e., for microfluidics or wound healing applications. With time, the surface wettability increases, and the scaffold can be populated by cells. Finally, the presence of GO allows for the use of infrared light for the sterilization of scaffolds and the disruption of any bacteria cell that might adhere to the more hydrophilic surface. Overall, our results showcase the potential of PCL-GO as a versatile material for medical applications.

生物医学应用的多功能支架:用富含氧化石墨烯的聚己内酯制作多功能解决方案。
医疗领域对多功能材料的迫切需求涵盖了各种情况,需要特定的组织功能。一个严峻的挑战是生物污损的发生,特别是手术环境中的污染,这是支架受损的常见原因。除了必须避免感染外,还必须将支架与活细胞结合,以便通过细胞附着实现组织再生。在此,我们将重点放在支架植入后的各种限时事件驱动下,开发一种用于医疗应用的多功能材料。我们研究了在聚己内酯(PCL)中加入氧化石墨烯(GO)的可能性,并创建了一种复合材料,用于三维打印具有时间可控的抗菌和抗粘连生长特性的支架。事实上,生产出的 PCL-GO 支架具有局部疏水效应,从而限制了生物实体的附着,包括降低了噬菌体的附着力,并使大肠杆菌和金黄色葡萄球菌的附着力分别降低了 81% 和 69%。此外,三维打印不同高度的 PCL-GO 支架还能控制细胞的分布和附着,这一特点也可用于细胞封闭,即微流控或伤口愈合应用。随着时间的推移,表面润湿性会增加,支架也会被细胞填充。最后,GO 的存在允许使用红外线对支架进行灭菌,并破坏可能附着在亲水性更强的表面上的任何细菌细胞。总之,我们的研究结果展示了 PCL-GO 作为一种多功能材料在医疗应用方面的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
APL Bioengineering
APL Bioengineering ENGINEERING, BIOMEDICAL-
CiteScore
9.30
自引率
6.70%
发文量
39
审稿时长
19 weeks
期刊介绍: APL Bioengineering is devoted to research at the intersection of biology, physics, and engineering. The journal publishes high-impact manuscripts specific to the understanding and advancement of physics and engineering of biological systems. APL Bioengineering is the new home for the bioengineering and biomedical research communities. APL Bioengineering publishes original research articles, reviews, and perspectives. Topical coverage includes: -Biofabrication and Bioprinting -Biomedical Materials, Sensors, and Imaging -Engineered Living Systems -Cell and Tissue Engineering -Regenerative Medicine -Molecular, Cell, and Tissue Biomechanics -Systems Biology and Computational Biology
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