Evaluation of rabbit adipose derived stem cells fate in perfused multilayered silk fibroin composite scaffold for Osteochondral repair

IF 3.2 4区医学 Q2 ENGINEERING, BIOMEDICAL

Journal of biomedical materials research. Part B, Applied biomaterials Pub Date : 2024-03-04 DOI:10.1002/jbm.b.35396

Sara Zadegan, Bahman Vahidi, Jhamak Nourmohammadi, Asiyeh Shojaee, Nooshin Haghighipour

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引用次数: 0

Abstract

Development of osteochondral tissue engineering approaches using scaffolds seeded with stem cells in association with mechanical stimulations has been recently considered as a promising technique for the repair of this tissue. In this study, an integrated and biomimetic trilayered silk fibroin (SF) scaffold containing SF nanofibers in each layer was fabricated. The osteogenesis and chondrogenesis of stem cells seeded on the fabricated scaffolds were investigated under a perfusion flow. 3-Dimethylthiazol-2,5-diphenyltetrazolium bromide assay showed that the perfusion flow significantly enhanced cell viability and proliferation. Analysis of gene expression by stem cells revealed that perfusion flow had significantly upregulated the expression of osteogenic and chondrogenic genes in the bone and cartilage layers and downregulated the hypertrophic gene expression in the intermediate layer of the scaffold. In conclusion, applying flow perfusion on the prepared integrated trilayered SF-based scaffold can support osteogenic and chondrogenic differentiation for repairing osteochondral defects.

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评估用于骨软骨修复的灌注多层丝纤维素复合支架中兔子脂肪衍生干细胞的命运。

最近，使用干细胞种子支架结合机械刺激开发骨软骨组织工程方法被认为是修复这种组织的一种有前途的技术。本研究制作了一种集成的仿生物三层丝纤维素（SF）支架，每层都含有SF纳米纤维。在灌注流条件下，研究了播种在所制支架上的干细胞的成骨和软骨发育情况。3-二甲基噻唑-2,5-二苯基溴化四氮唑试验表明，灌注流显著提高了细胞的活力和增殖。干细胞基因表达分析表明，灌注流明显提高了骨层和软骨层的成骨和软骨基因表达，降低了支架中间层的肥大基因表达。总之，在制备好的三层 SF 基集成支架上进行流动灌注可支持成骨和软骨分化，从而修复骨软骨缺损。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of biomedical materials research. Part B, Applied biomaterials 工程技术-材料科学：生物材料

CiteScore

7.50

自引率

2.90%

发文量

199

审稿时长

12 months

期刊介绍： Journal of Biomedical Materials Research – Part B: Applied Biomaterials is a highly interdisciplinary peer-reviewed journal serving the needs of biomaterials professionals who design, develop, produce and apply biomaterials and medical devices. It has the common focus of biomaterials applied to the human body and covers all disciplines where medical devices are used. Papers are published on biomaterials related to medical device development and manufacture, degradation in the body, nano- and biomimetic- biomaterials interactions, mechanics of biomaterials, implant retrieval and analysis, tissue-biomaterial surface interactions, wound healing, infection, drug delivery, standards and regulation of devices, animal and pre-clinical studies of biomaterials and medical devices, and tissue-biopolymer-material combination products. Manuscripts are published in one of six formats: • original research reports • short research and development reports • scientific reviews • current concepts articles • special reports • editorials Journal of Biomedical Materials Research – Part B: Applied Biomaterials is an official journal of the Society for Biomaterials, Japanese Society for Biomaterials, the Australasian Society for Biomaterials, and the Korean Society for Biomaterials. Manuscripts from all countries are invited but must be in English. Authors are not required to be members of the affiliated Societies, but members of these societies are encouraged to submit their work to the journal for consideration.