OfBFT genes play an essential role in the proliferate flower formation of Osmanthus fragrans

IF 5.7 2区生物学 Q1 PLANT SCIENCES

Plant Physiology and Biochemistry Pub Date : 2024-03-01 DOI:10.1016/j.plaphy.2024.108463

Qianqian Wang , Xin Chen , Xiaohan Liu , Ge Gao , Bin Dong , Yiguang Wang , Shiwei Zhong , Jinping Deng , Qiu Fang , Hongbo Zhao

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Abstract

Floral organ development is one of the most vital events in flowering plants and is closely related to ornamental properties. The proliferate flower (a new branch or flower occurring in the centre of a flower) in plants is an interesting type, while the specific molecular mechanism remains largely unknown. Osmanthus fragrans 'Tianxiang Taige' has two different flower morphologies: normal flower and proliferate flower. Phenotypic observation suggested that a normal flower was composed of calyx, petal, stamen and pistil (reduced to leaf-like carpel). While in proliferate flower, the leaf-like carpel continued to grow and was replaced by a new branch. Paraffin section indicated that the re-growth of leaf carpels might be the main reason for proliferate flower formation. Transcriptome sequencing of normal and proliferate flower was performed, and the expression levels of related genes were analysed. Among the differentially expressed genes, OfBFT-a and OfBFT-b had differential expression during the proliferate flower formation process. The expression patterns revealed that both OfBFT-a and OfBFT-b were highly accumulated in carpels, and were significantly downregulated during the proliferate flower development process. Subcellular localization indicated that OfBFT-a and OfBFT-b proteins were located in the nucleus. Functional studies in 'Tianxiang Taige' and Arabidopsis showed that OfBFT-a and OfBFT-b had important roles in floral organ development, especially the proliferate flower formation process by downregulating the accumulation of AG and SEP3 homologous genes. These results may shed new light on the study of proliferate flower formation and flower morphology breeding in flowering plants.

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OfBFT 基因在桂花的增殖花形成过程中发挥重要作用

花器官发育是开花植物中最重要的事件之一，与观赏性密切相关。植物的增殖花（在花的中心出现新的枝条或花朵）是一种有趣的类型，但其具体的分子机制仍不清楚。天香台阁'有两种不同的花形态：正常花和增生花。表型观察表明，正常花由花萼、花瓣、雄蕊和雌蕊（退化为叶状心皮）组成。而在增殖花中，叶状心皮继续生长，并被新的分枝取代。石蜡切片显示，叶状心皮的重新生长可能是增殖花形成的主要原因。对正常花和增殖花进行了转录组测序，并分析了相关基因的表达水平。在差异表达基因中，和在增殖花形成过程中具有差异表达。表达模式显示，和在心皮中高度积累，在催花过程中则显著下调。亚细胞定位表明，OfBFT-a 和 OfBFT-b 蛋白位于细胞核中。对'天香台阁'和的功能研究表明，和通过下调和同源基因的积累，在花器官发育，特别是催花形成过程中具有重要作用。这些结果可能会对有花植物的增殖花形成和花形态育种研究带来新的启示。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Plant Physiology and Biochemistry 生物-植物科学

CiteScore

11.10

自引率

3.10%

发文量

410

审稿时长

33 days

期刊介绍： Plant Physiology and Biochemistry publishes original theoretical, experimental and technical contributions in the various fields of plant physiology (biochemistry, physiology, structure, genetics, plant-microbe interactions, etc.) at diverse levels of integration (molecular, subcellular, cellular, organ, whole plant, environmental). Opinions expressed in the journal are the sole responsibility of the authors and publication does not imply the editors'' agreement. Manuscripts describing molecular-genetic and/or gene expression data that are not integrated with biochemical analysis and/or actual measurements of plant physiological processes are not suitable for PPB. Also "Omics" studies (transcriptomics, proteomics, metabolomics, etc.) reporting descriptive analysis without an element of functional validation assays, will not be considered. Similarly, applied agronomic or phytochemical studies that generate no new, fundamental insights in plant physiological and/or biochemical processes are not suitable for publication in PPB. Plant Physiology and Biochemistry publishes several types of articles: Reviews, Papers and Short Papers. Articles for Reviews are either invited by the editor or proposed by the authors for the editor''s prior agreement. Reviews should not exceed 40 typewritten pages and Short Papers no more than approximately 8 typewritten pages. The fundamental character of Plant Physiology and Biochemistry remains that of a journal for original results.