{"title":"A novel strategy to study apomixis, automixis, and autogamy in plants","authors":"Petra Šarhanová, Ľuboš Majeský, Michal Sochor","doi":"10.1007/s00497-024-00499-6","DOIUrl":null,"url":null,"abstract":"<h3 data-test=\"abstract-sub-heading\">Key message</h3><p>The combination of a flow cytometric seed screen and genotyping of each single seed offers a cost-effective approach to detecting complex reproductive pathways in flowering plants.</p><h3 data-test=\"abstract-sub-heading\">Abstract</h3><p>Reproduction may be seen as one of the driving forces of evolution. Flow cytometric seed screen and genotyping of parents and progeny are commonly employed techniques to discern various modes of reproduction in flowering plants. Nevertheless, both methods possess limitations constraining their individual capacity to investigate reproductive modes thoroughly. We implemented both methods in a novel manner to analyse reproduction pathways using a carefully selected material of parental individuals and their seed progeny. The significant advantage of this approach lies in its ability to apply both methods to a single seed. The introduced methodology provides valuable insights into discerning the levels of apomixis, sexuality, and selfing in complex <i>Rubus</i> taxa. The results may be explained by the occurrence of automixis in <i>Rubus</i>, which warrants further investigation. The approach showcased its effectiveness in a different apomictic system, specifically in <i>Taraxacum</i>. Our study presents a comprehensive methodological approach for determining the mode of reproduction where flow cytometry loses its potential. It provides a reliable and cost-effective method with significant potential in biosystematics, population genetics, and crop breeding.</p>","PeriodicalId":51297,"journal":{"name":"Plant Reproduction","volume":"183 1","pages":""},"PeriodicalIF":2.9000,"publicationDate":"2024-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Plant Reproduction","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s00497-024-00499-6","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0
Key message
The combination of a flow cytometric seed screen and genotyping of each single seed offers a cost-effective approach to detecting complex reproductive pathways in flowering plants.
Abstract
Reproduction may be seen as one of the driving forces of evolution. Flow cytometric seed screen and genotyping of parents and progeny are commonly employed techniques to discern various modes of reproduction in flowering plants. Nevertheless, both methods possess limitations constraining their individual capacity to investigate reproductive modes thoroughly. We implemented both methods in a novel manner to analyse reproduction pathways using a carefully selected material of parental individuals and their seed progeny. The significant advantage of this approach lies in its ability to apply both methods to a single seed. The introduced methodology provides valuable insights into discerning the levels of apomixis, sexuality, and selfing in complex Rubus taxa. The results may be explained by the occurrence of automixis in Rubus, which warrants further investigation. The approach showcased its effectiveness in a different apomictic system, specifically in Taraxacum. Our study presents a comprehensive methodological approach for determining the mode of reproduction where flow cytometry loses its potential. It provides a reliable and cost-effective method with significant potential in biosystematics, population genetics, and crop breeding.
期刊介绍:
Plant Reproduction (formerly known as Sexual Plant Reproduction) is a journal devoted to publishing high-quality research in the field of reproductive processes in plants. Article formats include original research papers, expert reviews, methods reports and opinion papers. Articles are selected based on significance for the field of plant reproduction, spanning from the induction of flowering to fruit development. Topics incl … show all