A novel strategy to study apomixis, automixis, and autogamy in plants

IF 2.9 4区 生物学 Q2 PLANT SCIENCES
Petra Šarhanová, Ľuboš Majeský, Michal Sochor
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Key message

The combination of a flow cytometric seed screen and genotyping of each single seed offers a cost-effective approach to detecting complex reproductive pathways in flowering plants.

Abstract

Reproduction may be seen as one of the driving forces of evolution. Flow cytometric seed screen and genotyping of parents and progeny are commonly employed techniques to discern various modes of reproduction in flowering plants. Nevertheless, both methods possess limitations constraining their individual capacity to investigate reproductive modes thoroughly. We implemented both methods in a novel manner to analyse reproduction pathways using a carefully selected material of parental individuals and their seed progeny. The significant advantage of this approach lies in its ability to apply both methods to a single seed. The introduced methodology provides valuable insights into discerning the levels of apomixis, sexuality, and selfing in complex Rubus taxa. The results may be explained by the occurrence of automixis in Rubus, which warrants further investigation. The approach showcased its effectiveness in a different apomictic system, specifically in Taraxacum. Our study presents a comprehensive methodological approach for determining the mode of reproduction where flow cytometry loses its potential. It provides a reliable and cost-effective method with significant potential in biosystematics, population genetics, and crop breeding.

Abstract Image

研究植物无花果混交、自动混交和自交的新策略
关键信息流式细胞种子筛选与每粒种子的基因分型相结合,为检测开花植物的复杂繁殖途径提供了一种经济有效的方法。 摘要繁殖可被视为进化的驱动力之一。流式细胞种子筛选和亲本与后代基因分型是鉴别有花植物各种繁殖模式的常用技术。然而,这两种方法都有一定的局限性,限制了它们各自深入研究繁殖模式的能力。我们以一种新颖的方式实施了这两种方法,利用精心挑选的亲本个体及其种子后代材料来分析繁殖途径。这种方法的显著优势在于它能将这两种方法应用于一粒种子。引入的方法为辨别复杂茜草类群的无性繁殖、有性生殖和自交水平提供了宝贵的见解。这些结果可能与茜草中出现的自交现象有关,值得进一步研究。该方法在不同的无性生殖系统中,特别是在蒲公英中显示了其有效性。我们的研究提出了一种全面的方法,可用于确定流式细胞仪无法发挥其潜力的繁殖模式。它提供了一种可靠、经济有效的方法,在生物系统学、群体遗传学和作物育种方面具有巨大潜力。
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来源期刊
Plant Reproduction
Plant Reproduction PLANT SCIENCES-REPRODUCTIVE BIOLOGY
CiteScore
6.30
自引率
2.90%
发文量
19
期刊介绍: Plant Reproduction (formerly known as Sexual Plant Reproduction) is a journal devoted to publishing high-quality research in the field of reproductive processes in plants. Article formats include original research papers, expert reviews, methods reports and opinion papers. Articles are selected based on significance for the field of plant reproduction, spanning from the induction of flowering to fruit development. Topics incl … show all
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