Identification of plb1 mutation that extends longevity via activating Sty1 MAPK in Schizosaccharomyces pombe.

IF 2.3 3区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Yasukichi Maekawa, Kotaro Matsui, Keisuke Okamoto, Takafumi Shimasaki, Hokuto Ohtsuka, Motohiro Tani, Kunio Ihara, Hirofumi Aiba
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Abstract

To understand the lifespan of higher organisms, including humans, it is important to understand lifespan at the cellular level as a prerequisite. So, fission yeast is a good model organism for the study of lifespan. To identify the novel factors involved in longevity, we are conducting a large-scale screening of long-lived mutant strains that extend chronological lifespan (cell survival in the stationary phase) using fission yeast. One of the newly acquired long-lived mutant strains (No.98 mutant) was selected for analysis and found that the long-lived phenotype was due to a missense mutation (92Phe → Ile) in the plb1+ gene. plb1+ gene in fission yeast is a nonessential gene encoding a homolog of phospholipase B, but its functions under normal growth conditions, as well as phospholipase B activity, remain unresolved. Our analysis of the No.98 mutant revealed that the plb1 mutation reduces the integrity of the cellular membrane and cell wall and activates Sty1 via phosphorylation.

鉴定可通过激活 Sty1 MAPK 延长鼠李酵母寿命的 plb1 突变。
要了解包括人类在内的高等生物的寿命,必须以了解细胞水平的寿命为前提。因此,裂殖酵母是研究寿命的良好模式生物。为了找出参与长寿的新因素,我们正在利用裂殖酵母大规模筛选延长计时寿命(细胞在静止期存活)的长寿突变株。我们选择了其中一个新获得的长寿突变株(No.98 突变株)进行分析,发现其长寿表型是由 plb1+ 基因中的一个错义突变(92Phe → Ile)引起的。裂殖酵母中的 plb1+ 基因是一个编码磷脂酶 B 同源物的非必要基因,但其在正常生长条件下的功能以及磷脂酶 B 的活性仍未得到解决。我们对 No.98 突变体的分析表明,plb1 突变会降低细胞膜和细胞壁的完整性,并通过磷酸化激活 Sty1。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Molecular Genetics and Genomics
Molecular Genetics and Genomics 生物-生化与分子生物学
CiteScore
5.10
自引率
3.20%
发文量
134
审稿时长
1 months
期刊介绍: Molecular Genetics and Genomics (MGG) publishes peer-reviewed articles covering all areas of genetics and genomics. Any approach to the study of genes and genomes is considered, be it experimental, theoretical or synthetic. MGG publishes research on all organisms that is of broad interest to those working in the fields of genetics, genomics, biology, medicine and biotechnology. The journal investigates a broad range of topics, including these from recent issues: mechanisms for extending longevity in a variety of organisms; screening of yeast metal homeostasis genes involved in mitochondrial functions; molecular mapping of cultivar-specific avirulence genes in the rice blast fungus and more.
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