Elevated MIF identified by multiple cytokine analyses facilitates macrophage M2 polarization contributing to postoperative recurrence in chronic rhinosinusitis with nasal polyps.

IF 4.8 2区医学 Q1 OTORHINOLARYNGOLOGY

Rhinology Pub Date : 2024-08-01 DOI:10.4193/Rhin23.412

S Xie, Z Tong, J Zhang, C Yang, W Jiang, H Zhang

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引用次数: 0

Abstract

Background: Chronic rhinosinusitis with nasal polyps (CRSwNP) is characterized by tissue heterogeneity and high postoperative recurrence risk. This study aims to employ cytokine analyses to identify serum biomarkers associated with postoperative CRSwNP recurrence and elucidate underlying recurrent mechanisms.

Methods: A prospective cohort study was conducted on CRSwNP patients undergoing functional endoscopic sinus surgery. Serum and tissue samples were collected and analyzed for multiple cytokines. Participants were followed for 3 years and categorized into recurrent and non-recurrent groups. Cytokine profiles were compared, and potential markers for recurrence were further assessed. Macrophage migration inhibitory factor (MIF) expression in macrophages was modulated, and their polarization and cytokine secretion were assessed.

Results: In the discovery cohort (21 recurrent and 40 non-recurrent patients), circulating cytokine profiles differed significantly, with 8 cytokines showing differential expression between the two groups. Among them, serum eotaxin, MIF, RANTES, and TRAIL exhibited promise in predicting recurrence. In the validation cohort (24 recurrent and 44 non-recurrent patients), serum eotaxin, MIF, and TRAIL levels were higher in recurrent cases. Tissue MIF was elevated in recurrent cases and had a strong predictive value for recurrence. Moreover, tissue MIF was co-expressed with CD206 in recurrent cases. Mechanistically, MIF overexpression promoted macrophage M2 polarization and TGF-β, CCL-24, and MIF secretion, and MIF recombinant protein facilitated M2 polarization, and TGF-β1 and CCL-24 production, contributing to CRSwNP recurrence.

Conclusions: Serum-specific cytokine signatures were associated with postoperative recurrence risk in CRSwNP. Elevated MIF enhanced macrophage M2 polarization and cytokine secretion, contributing to the recurrent mechanisms of CRSwNP.

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通过多种细胞因子分析确定的 MIF 升高促进了巨噬细胞 M2 极化，导致伴有鼻息肉的慢性鼻窦炎患者术后复发。

背景：慢性鼻炎伴鼻息肉（CRSwNP）的特点是组织异质性和术后复发风险高。本研究旨在利用细胞因子分析确定与 CRSwNP 术后复发相关的血清生物标志物，并阐明潜在的复发机制：方法：对接受功能性内窥镜鼻窦手术的CRSwNP患者进行前瞻性队列研究。收集血清和组织样本并分析多种细胞因子。研究人员对参与者进行了为期 3 年的随访，并将其分为复发组和非复发组。对细胞因子谱进行比较，并进一步评估复发的潜在标志物。对巨噬细胞中巨噬细胞迁移抑制因子（MIF）的表达进行了调节，并对其极化和细胞因子分泌情况进行了评估：结果：在发现队列（21 名复发患者和 40 名非复发患者）中，循环细胞因子谱存在显著差异，其中 8 种细胞因子在两组患者中的表达存在差异。其中，血清 eotaxin、MIF、RANTES 和 TRAIL 具有预测复发的前景。在验证队列（24 名复发患者和 44 名非复发患者）中，复发病例的血清 eotaxin、MIF 和 TRAIL 水平较高。组织 MIF 在复发病例中升高，对复发有很强的预测价值。此外，在复发病例中，组织 MIF 与 CD206 共同表达。从机理上讲，MIF过表达促进巨噬细胞M2极化和TGF-β1、CCL-24及MIF分泌，MIF重组蛋白促进M2极化、TGF-β1和CCL-24分泌，从而导致CRSwNP复发：结论：血清特异性细胞因子特征与CRSwNP术后复发风险有关。MIF的升高增强了巨噬细胞M2极化和细胞因子分泌，有助于CRSwNP的复发机制。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Rhinology 医学-耳鼻喉科学

CiteScore

15.80

自引率

9.70%

发文量

135

审稿时长

6-12 weeks

期刊介绍： Rhinology serves as the official Journal of the International Rhinologic Society and is recognized as one of the journals of the European Rhinologic Society. It offers a prominent platform for disseminating rhinologic research, reviews, position papers, task force reports, and guidelines to an international scientific audience. The journal also boasts the prestigious European Position Paper in Rhinosinusitis (EPOS), a highly influential publication first released in 2005 and subsequently updated in 2007, 2012, and most recently in 2020. Employing a double-blind peer review system, Rhinology welcomes original articles, review articles, and letters to the editor.