The GaKAN2, a KANADI transcription factor, modulates stem trichomes in Gossypium arboreum.

IF 2.3 3区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Xujiao Ren, Luying Yang, Yasir Muhammad, Yuxing Xie, Xinyi Lin, Li Yu, Yuefen Cao, Mingquan Ding, Yurong Jiang, Junkang Rong
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引用次数: 0

Abstract

Key message: GaKAN2, a member of the KANADI family, was found to be widely expressed in the cotton tissues and regulates trichome development through complex pathways. Cotton trichomes are believed to be the defense barrier against insect pests. Cotton fiber and trichomes are single-cell epidermal extensions with shared regulatory mechanisms. Despite several studies underlying mechanism of trichome development remains elusive. The KANADI is one of the key transcription factors (TFs) family, regulating Arabidopsis trichomes growth. However, the function of KANADI genes in cotton remains unknown. In the current study genome-wide scanning, transcriptomic analysis, gene silencing, subcellular localization, and yeast two-hybrid techniques were employed to decipher the function of KANADI TFs family genes in cotton crop. A total of 7 GaKAN genes were found in the Gossypium arboreum. Transcriptomic data revealed that these genes were significantly expressed in stem and root. Moreover, GaKAN2 was widely expressed in other tissues also. Subsequently, we selected GaKAN2 to validate the function of KANADI genes. Silencing of GaKAN2 resulted in a 24.99% decrease in single-cell trichomes and an 11.33% reduction in internodal distance, indicating its potential role in regulating trichomes and plant growth. RNA-Seq analysis elucidated that GaSuS and GaERS were the downstream genes of GaKAN2. The transcriptional activation and similarity in silencing phenotype between GaKAN2 and GaERS suggested that GaKAN2 regulates trichomes development through GaERS. Moreover, KEGG analysis revealed that a significant number of genes were enriched in the biosynthesis of secondary metabolites and plant hormone signal transduction pathways, thereby suggesting that GaKAN2 regulates the stem trichomes and plant growth. The GFP subcellular localization and yeast transcriptional activation analysis elucidated that GaKAN2 was located in the nucleus and capable of regulating the transcription of downstream genes. This study elucidated the function and characteristics of the KANADI gene family in cotton, providing a fundamental basis for further research on GaKAN2 gene in cotton plant trichomes and plant developmental processes.

Abstract Image

KANADI转录因子GaKAN2调节旱生麻的茎毛。
关键信息研究发现,KANADI 家族成员 GaKAN2 在棉花组织中广泛表达,并通过复杂的途径调控毛状体的发育。棉花毛状体被认为是抵御害虫的屏障。棉纤维和毛状体是单细胞表皮延伸,具有共同的调控机制。尽管进行了多项研究,但毛状体发育的基本机制仍然难以捉摸。KANADI 是拟南芥毛状体生长的关键转录因子(TFs)家族之一。然而,KANADI基因在棉花中的功能仍然未知。本研究采用全基因组扫描、转录组分析、基因沉默、亚细胞定位和酵母双杂交等技术,对棉花作物中KANADI TFs家族基因的功能进行了解读。研究发现,棉花中共有 7 个 GaKAN 基因。转录组数据显示,这些基因在茎和根中有显著表达。此外,GaKAN2 也在其他组织中广泛表达。随后,我们选择了 GaKAN2 来验证 KANADI 基因的功能。沉默GaKAN2后,单细胞毛状体减少了24.99%,节间距离减少了11.33%,这表明它在调控毛状体和植物生长中的潜在作用。RNA-Seq分析表明,GaSuS和GaERS是GaKAN2的下游基因。GaKAN2和GaERS的转录激活和沉默表型相似,表明GaKAN2通过GaERS调控毛状体的发育。此外,KEGG分析显示,大量基因富集在次生代谢产物的生物合成和植物激素信号转导途径中,从而表明GaKAN2调控茎毛状体和植物生长。GFP亚细胞定位和酵母转录激活分析阐明,GaKAN2位于细胞核内,能够调控下游基因的转录。该研究阐明了棉花中KANADI基因家族的功能和特点,为进一步研究GaKAN2基因在棉花植物毛状体和植物发育过程中的作用提供了基础。
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来源期刊
Molecular Genetics and Genomics
Molecular Genetics and Genomics 生物-生化与分子生物学
CiteScore
5.10
自引率
3.20%
发文量
134
审稿时长
1 months
期刊介绍: Molecular Genetics and Genomics (MGG) publishes peer-reviewed articles covering all areas of genetics and genomics. Any approach to the study of genes and genomes is considered, be it experimental, theoretical or synthetic. MGG publishes research on all organisms that is of broad interest to those working in the fields of genetics, genomics, biology, medicine and biotechnology. The journal investigates a broad range of topics, including these from recent issues: mechanisms for extending longevity in a variety of organisms; screening of yeast metal homeostasis genes involved in mitochondrial functions; molecular mapping of cultivar-specific avirulence genes in the rice blast fungus and more.
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