Application of noninvasive sampling technique in mitochondrial genome intraspecific phylogeny of the endangered butterfly, Teinopalpus aureus (Lepidoptera: Papilionidae).
{"title":"Application of noninvasive sampling technique in mitochondrial genome intraspecific phylogeny of the endangered butterfly, Teinopalpus aureus (Lepidoptera: Papilionidae).","authors":"Wen-Jing Yang, Gui-Qiang He, Chao-Bin Huang, Shan-Yi Zhou, Feng-Hai Jia, Ju-Ping Zeng","doi":"10.1093/jisesa/ieae008","DOIUrl":null,"url":null,"abstract":"<p><p>The butterfly genus of Teinopalpus, endemic to Asia, embodies a distinct species of mountain-dwelling butterflies with specific habitat requirements. These species are rare in the wild and hold high conservation and research value. Similar to other protected species, the genetic analysis of the rare Teinopalpus aureus poses challenges due to the complexity of sampling. In this study, we successfully extracted DNA and amplified mitochondrial genomic DNA from various noninvasive sources such as larval feces, larval exuviae, larval head capsules, pupal exuviaes, and filamentous gland secretions, all integral parts of butterfly metamorphosis. This was conducted as part of a research initiative focused on the artificial conservation of T. aureus population in Jinggang Shan Nature Reserve. Our findings illustrated the successful extraction of DNA from multiple noninvasive sources, achieved through modified DNA extraction methodologies. Although the DNA concentration obtained from noninvasive samples was lower than that from muscle tissues of newly dead larvae during rearing, all samples met the requirements for PCR amplification and sequencing, yielding complete circular sequences. These sequences are pivotal for both interspecific and intraspecific genetic relationship analysis. Our methods can be extended to other insects, especially scarce species.</p>","PeriodicalId":16156,"journal":{"name":"Journal of Insect Science","volume":null,"pages":null},"PeriodicalIF":2.1000,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10898791/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Insect Science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1093/jisesa/ieae008","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENTOMOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
The butterfly genus of Teinopalpus, endemic to Asia, embodies a distinct species of mountain-dwelling butterflies with specific habitat requirements. These species are rare in the wild and hold high conservation and research value. Similar to other protected species, the genetic analysis of the rare Teinopalpus aureus poses challenges due to the complexity of sampling. In this study, we successfully extracted DNA and amplified mitochondrial genomic DNA from various noninvasive sources such as larval feces, larval exuviae, larval head capsules, pupal exuviaes, and filamentous gland secretions, all integral parts of butterfly metamorphosis. This was conducted as part of a research initiative focused on the artificial conservation of T. aureus population in Jinggang Shan Nature Reserve. Our findings illustrated the successful extraction of DNA from multiple noninvasive sources, achieved through modified DNA extraction methodologies. Although the DNA concentration obtained from noninvasive samples was lower than that from muscle tissues of newly dead larvae during rearing, all samples met the requirements for PCR amplification and sequencing, yielding complete circular sequences. These sequences are pivotal for both interspecific and intraspecific genetic relationship analysis. Our methods can be extended to other insects, especially scarce species.
蝶属(Teinopalpus)是亚洲特有的蝴蝶属,拥有独特的山栖蝴蝶物种,对栖息地有特殊要求。这些物种在野外十分罕见,具有很高的保护和研究价值。与其他受保护的物种类似,由于取样的复杂性,对稀有的金龟子蝶进行遗传分析也是一项挑战。在这项研究中,我们成功地从幼虫粪便、幼虫卵壳、幼虫头囊、蛹的卵壳和丝状腺分泌物等各种非侵入性来源中提取了DNA,并扩增了线粒体基因组DNA,这些都是蝴蝶变态过程中不可或缺的部分。这项研究是井冈山自然保护区蝶类种群人工保护研究的一部分。我们的研究结果表明,通过改进 DNA 提取方法,我们成功地从多个非侵入性来源提取了 DNA。虽然非入侵样本的DNA浓度低于饲养过程中新死亡幼虫肌肉组织的DNA浓度,但所有样本都符合PCR扩增和测序的要求,获得了完整的环状序列。这些序列对于分析种间和种内遗传关系至关重要。我们的方法可以推广到其他昆虫,尤其是稀缺物种。
期刊介绍:
The Journal of Insect Science was founded with support from the University of Arizona library in 2001 by Dr. Henry Hagedorn, who served as editor-in-chief until his death in January 2014. The Entomological Society of America was very pleased to add the Journal of Insect Science to its publishing portfolio in 2014. The fully open access journal publishes papers in all aspects of the biology of insects and other arthropods from the molecular to the ecological, and their agricultural and medical impact.