Antagonistic Effect of Zinc Oxide Nanoparticles Dietary Supplementation Against Chronic Copper Waterborne Exposure on Growth, Behavioral, Biochemical, and Gene Expression Alterations of African Catfish, Clarias gariepinus (Burchell, 1822).

IF 3.4 3区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Biological Trace Element Research Pub Date : 2024-12-01 Epub Date: 2024-02-28 DOI:10.1007/s12011-024-04115-6
Shaimaa A A Ahmed, Rowida E Ibrahim, Elsayed M Younis, Abdelwahab A Abdelwarith, Khaled Yehia Faroh, Samar A El Gamal, Shereen Badr, Tarek Khamis, Abdallah Tageldein Mansour, Simon J Davies, Mohamed ElHady
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Abstract

The harmful impact of waterborne copper (Cu) as a common abiotic stressor in aquatic environments has gained much more interest. The present study aimed to investigate the utilization of zinc oxide nanoparticles (ZnONPs) dietary supplementation to mitigate the chronic toxicity of Cu in African catfish (Clarias gariepinus). Two hundred and forty fish (92.94 ± 0.13 g) were assigned into six groups for 60 days. Control (C), ZnONPs20, and ZnONPs30 groups were fed on basal diets fortified with 0, 20, and 30 mg kg-1 ZnONPs without Cu exposure. Cu, Cu + ZnONPs20, and Cu + ZnONPs30 groups were exposed to Cu at a dose of 10 mg L-1 and fed on basal diets fortified with 0, 20, and 30 mg kg-1 ZnONPs, respectively. The results revealed that the Cu-exposed fish experienced abnormal clinical signs and behavioral changes. The growth indices and acetylcholine esterase activity were significantly decreased (P < 0.05) in the Cu group. Meanwhile, hepatorenal and serum stress indices (P < 0.05) were significantly elevated with chronic Cu exposure. In addition, a higher expression of stress (P < 0.05) (heat shock protein 60 and hypoxia-inducible factor-1 alpha) and apoptotic-related genes (C/EBP homologous protein, caspase-3, and Bcl-2 Associated X-protein) with down-regulation (P < 0.05) of the anti-apoptotic-related genes (B-cell lymphoma 2 and proliferating cell nuclear antigen) was noticed in the Cu-exposed fish. Histopathological alterations in the gills, liver, kidney, and spleen were markedly reported in the Cu-exposed group. The dietary supplementation with ZnONPs significantly alleviated the negative impacts of chronic waterborne-Cu exposure on growth performance, physiological changes, gene expression, and tissue architecture, especially at 30 mg kg-1 diet level. In particular, the inclusion of ZnONPs at the 30 mg kg-1 diet level produced better outcomes than the 20 mg kg-1 diet. Overall, ZnONPs could be added as a feed supplement in the C. gariepinus diet to boost the fish's health and productivity and alleviate the stress condition brought on by Cu exposure.

纳米氧化锌膳食补充剂对非洲鲶鱼(Clarias gariepinus (Burchell, 1822))生长、行为、生化和基因表达改变的拮抗作用。
水载铜(Cu)是水生环境中常见的非生物压力源,其有害影响已引起越来越多的关注。本研究旨在探讨利用纳米氧化锌(ZnONPs)膳食补充剂来减轻铜对非洲鲶(Clarias gariepinus)的慢性毒性。将 240 尾鱼(92.94 ± 0.13 克)分成 6 组,每组 60 天。对照组(C)、ZnONPs20 组和 ZnONPs30 组在不接触铜的情况下喂食添加了 0、20 和 30 mg kg-1 ZnONPs 的基础日粮。Cu、Cu + ZnONPs20 和 Cu + ZnONPs30 组暴露于 10 mg L-1 剂量的铜,并分别饲喂添加了 0、20 和 30 mg kg-1 ZnONPs 的基础日粮。结果显示,暴露于铜的鱼出现了异常的临床症状和行为变化。生长指数和乙酰胆碱酯酶活性显著下降(P -1 日粮水平)。特别是,在 30 毫克/千克的日粮水平上添加 ZnONPs 比在 20 毫克/千克的日粮水平上添加 ZnONPs 产生的效果更好。总之,在嘉鱼的日粮中添加 ZnONPs 作为饲料添加剂,可提高鱼类的健康水平和生产率,缓解铜暴露带来的应激状况。
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来源期刊
Biological Trace Element Research
Biological Trace Element Research 生物-内分泌学与代谢
CiteScore
8.70
自引率
10.30%
发文量
459
审稿时长
2 months
期刊介绍: Biological Trace Element Research provides a much-needed central forum for the emergent, interdisciplinary field of research on the biological, environmental, and biomedical roles of trace elements. Rather than confine itself to biochemistry, the journal emphasizes the integrative aspects of trace metal research in all appropriate fields, publishing human and animal nutritional studies devoted to the fundamental chemistry and biochemistry at issue as well as to the elucidation of the relevant aspects of preventive medicine, epidemiology, clinical chemistry, agriculture, endocrinology, animal science, pharmacology, microbiology, toxicology, virology, marine biology, sensory physiology, developmental biology, and related fields.
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