Embryonic Amoxicillin Exposure Has Limited Impact on Liver Development but Increases Susceptibility to NAFLD in Zebrafish Larvae

IF 4.9 2区生物学

International Journal of Molecular Sciences Pub Date : 2024-02-27 DOI:10.3390/ijms25052744

Peng Gao, Cheng Chang, Jieling Liang, Fen Du, Ruilin Zhang

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引用次数: 0

Abstract

Amoxicillin is commonly used in clinical settings to target bacterial infection and is frequently prescribed during pregnancy. Investigations into its developmental toxicity and effects on disease susceptibility are not comprehensive. Our present study examined the effects of embryonic amoxicillin exposure on liver development and function, especially the effects on susceptibility to non-alcoholic fatty liver disease (NAFLD) using zebrafish as an animal model. We discovered that embryonic amoxicillin exposure did not compromise liver development, nor did it induce liver toxicity. However, co-treatment of amoxicillin and clavulanic acid diminished BESP expression, caused bile stasis and induced liver toxicity. Embryonic amoxicillin exposure resulted in elevated expression of lipid synthesis genes and exacerbated hepatic steatosis in a fructose-induced NAFLD model, indicating embryonic amoxicillin exposure increased susceptibility to NAFLD in zebrafish larvae. In summary, this research broadens our understanding of the risks of amoxicillin usage during pregnancy and provides evidence for the impact of embryonic amoxicillin exposure on disease susceptibility in offspring.

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胚胎期暴露于阿莫西林对斑马鱼幼体的肝脏发育影响有限，但会增加非酒精性脂肪肝的易感性

阿莫西林是临床上常用的治疗细菌感染的药物，也是孕期常用的处方药。有关其发育毒性和对疾病易感性影响的研究并不全面。本研究以斑马鱼为动物模型，研究了胚胎期接触阿莫西林对肝脏发育和功能的影响，尤其是对非酒精性脂肪肝易感性的影响。我们发现，胚胎期接触阿莫西林不会影响肝脏发育，也不会诱发肝脏毒性。然而，阿莫西林和克拉维酸联合治疗会降低BESP的表达，导致胆汁淤积并诱发肝脏毒性。在果糖诱导的非酒精性脂肪肝模型中，胚胎期暴露于阿莫西林会导致脂质合成基因表达升高，加重肝脏脂肪变性，这表明胚胎期暴露于阿莫西林会增加斑马鱼幼体对非酒精性脂肪肝的易感性。总之，这项研究拓宽了我们对孕期使用阿莫西林风险的认识，并为胚胎期接触阿莫西林对后代疾病易感性的影响提供了证据。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

International Journal of Molecular Sciences 化学-化学综合

自引率

10.70%

发文量

13472

审稿时长

1.7 months

期刊介绍： The International Journal of Molecular Sciences (ISSN 1422-0067) provides an advanced forum for chemistry, molecular physics (chemical physics and physical chemistry) and molecular biology. It publishes research articles, reviews, communications and short notes. Our aim is to encourage scientists to publish their theoretical and experimental results in as much detail as possible. Therefore, there is no restriction on the length of the papers or the number of electronics supplementary files. For articles with computational results, the full experimental details must be provided so that the results can be reproduced. Electronic files regarding the full details of the calculation and experimental procedure, if unable to be published in a normal way, can be deposited as supplementary material (including animated pictures, videos, interactive Excel sheets, software executables and others).