Adrenal venous sampling criteria for chemiluminescent enzyme immunoassay as a preferable alternative to radioimmunoassay in primary aldosteronism

IF 1.3 4区 医学 Q4 ENDOCRINOLOGY & METABOLISM
Kazuki Nakai, Yuya Tsurutani, Koki Irie, Kyoko Teruyama, Sachiko Suematsu, Seishi Matsui, Kohzoh Makita, Jun Saito, Masao Omura, Tetsuo Nishikawa
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Abstract

Plasma aldosterone concentration (PAC) was routinely measured using radioimmunoassay (RIA); however, the RIA kit was discontinued in March 2021 in Japan. This study examined PAC conversion in adrenal venous sampling (AVS) and AVS criteria when measured using chemiluminescent enzyme immunoassay (CLEIA). PAC of 415 adrenal venous blood samples from AVS (including segmental AVS) of 63 patients with primary aldosteronism was measured using RIA (Spac-S aldosterone kit; Fujirebio Inc.) and CLEIA (Lumipulse Presto Aldosterone; Fujirebio Inc.). PAC of 70 AVS samples was also measured using liquid chromatography-mass spectrometry (LC-MS/MS, ASKA Pharma Medical Co., Ltd.). PAC conversion formulas were determined for each AVS sample assay. PAC measured using CLEIA was significantly correlated with that measured using RIA (correlation coefficient = 0.971). The PAC conversion formula was PAC (CLEIA) = PAC (RIA) × 0.772 – 1,199 pg/mL. The PAC of 14,000 pg/mL in RIA was equivalent to 9,613 pg/mL in CLEIA. PAC measured using CLEIA was also correlated with that measured using LC-MS/MS, and the PAC conversion formula was PAC (CLEIA, pg/mL) = 0.97 × PAC (LC-MS/MS, pg/mL) + 211. The inter-assay coefficient of variability (CV) was 1.1–1.3% and intra-assay CV was 1.0–1.7%, measured using CLEIA. The PAC conversion formula for AVS samples was obtained using CLEIA and RIA, and the conversion formula was different from that for peripheral blood. PAC values measured by CLEIA showed preferable accuracy and high concordance with those measured by LC-MS/MS, even in AVS samples. The study outcomes are useful for interpreting AVS results using non-RIA measurement methods.

原发性醛固酮增多症中化学发光酶免疫测定优于放射免疫测定的肾上腺静脉取样标准
血浆醛固酮浓度 (PAC) 的常规测量方法是放射免疫分析法 (RIA);然而,RIA 试剂盒已于 2021 年 3 月在日本停产。本研究考察了肾上腺静脉采样(AVS)中 PAC 的转换情况以及使用化学发光酶免疫分析法(CLEIA)测量的 AVS 标准。研究使用 RIA(Spac-S 醛固酮试剂盒;Fujirebio Inc.)和 CLEIA(Lumipulse Presto Aldosterone;Fujirebio Inc.)测定了 63 名原发性醛固酮增多症患者的 415 份肾上腺静脉血样本(包括节段性 AVS)的 PAC。此外,还使用液相色谱-质谱法(LC-MS/MS,ASKA Pharma Medical Co.)为每种 AVS 样品测定确定了 PAC 换算公式。使用 CLEIA 测得的 PAC 与使用 RIA 测得的 PAC 显著相关(相关系数 = 0.971)。PAC 换算公式为 PAC (CLEIA) = PAC (RIA) × 0.772 - 1,199 pg/mL。RIA 中 14,000 pg/mL 的 PAC 相当于 CLEIA 中的 9,613 pg/mL。使用 CLEIA 测得的 PAC 也与使用 LC-MS/MS 测得的 PAC 相关,PAC 换算公式为 PAC(CLEIA,pg/mL)= 0.97 × PAC(LC-MS/MS,pg/mL)+ 211。使用 CLEIA 测得的测定间变异系数 (CV) 为 1.1-1.3%,测定内变异系数为 1.0-1.7%。使用 CLEIA 和 RIA 获得了 AVS 样品的 PAC 换算公式,其换算公式与外周血不同。通过 CLEIA 测得的 PAC 值与通过 LC-MS/MS 测得的 PAC 值具有更高的准确性和一致性,即使在 AVS 样品中也是如此。研究结果有助于解释使用非 RIA 测量方法得出的 AVS 结果。
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来源期刊
Endocrine journal
Endocrine journal 医学-内分泌学与代谢
CiteScore
4.30
自引率
5.00%
发文量
224
审稿时长
1.5 months
期刊介绍: Endocrine Journal is an open access, peer-reviewed online journal with a long history. This journal publishes peer-reviewed research articles in multifaceted fields of basic, translational and clinical endocrinology. Endocrine Journal provides a chance to exchange your ideas, concepts and scientific observations in any area of recent endocrinology. Manuscripts may be submitted as Original Articles, Notes, Rapid Communications or Review Articles. We have a rapid reviewing and editorial decision system and pay a special attention to our quick, truly scientific and frequently-citable publication. Please go through the link for author guideline.
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