High efficacy fluconazole loaded ZnO-poly (vinyl alcohol) nanocomposite: Interpretive breakpoints for biological applications

IF 3.8 4区工程技术 Q2 CHEMISTRY, APPLIED

Journal of Vinyl & Additive Technology Pub Date : 2024-02-27 DOI:10.1002/vnl.22098

Abinash Das, Togam Ringu, Sampad Ghosh, Nabakumar Pramanik

{"title":"High efficacy fluconazole loaded ZnO-poly (vinyl alcohol) nanocomposite: Interpretive breakpoints for biological applications","authors":"Abinash Das, Togam Ringu, Sampad Ghosh, Nabakumar Pramanik","doi":"10.1002/vnl.22098","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <p>Nanotechnology, a versatile field, holds promise in diverse applications, such as advanced pharmaceutical techniques and innovative chemical compound fabrication. Recently, the World Health Organization (WHO) has identified sepsis as a global health priority, attributing most sepsis-related deaths to the underlying infection. Sepsis is a complex disease that manifests in various ways, depending on factors, such as pathogen involved, mode of transmission, and the patient's immune competence. This study focuses on synthesizing zinc oxide (ZnO) through an in-situ precipitation method and employing a solution-based technique to coat the inorganic ZnO nanomaterial with the antimicrobial drug fluconazole (FLZ), resulting in a FLZ-ZnO composite. Further enhancement is achieved by modifying the composite with poly(vinyl alcohol) (PVA) to improve mechanical strength, physicochemical characteristics, and the interfacial network between ZnO and FLZ. Characterization through X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), field emission scanning electron microscopy (FESEM), particle size distribution (PSD), and thermo gravimetric analysis (TGA) confirms the synthesized compounds are stoichiometric in nature. The FLZ-ZnO-PVA nanocomposite demonstrates significant antifungal activity against <i>C. albicans</i> and <i>A. niger</i>, as well as enhanced antibacterial activity against <i>E. coli</i> and <i>S. aureus</i> evaluated through well diffusion technique. In vitro cellular compatibility assessment using the MTT assay with NIH-3T3 cells reveals exceptional viability (above 75%) and negligible cytotoxicity at a concentration of 1.56 μg/mL, indicating high biosafety. The FLZ-ZnO-PVA nanocomposite exhibits outstanding biological performance, making it a promising candidate for clinical applications in preventing sepsis and prospective infections.</p>\n </section>\n \n <section>\n \n <h3> Highlights</h3>\n \n <div>\n <ul>\n \n <li>Developments and preparation of FLZ-ZnO-PVA nanocomposite.</li>\n \n <li>FLZ-ZnO-PVA nanocomposite shows optimum antimicrobial activity.</li>\n \n <li>FLZ-ZnO-PVA shows cytotoxicity against the mouse embryonic fibroblast cell line.</li>\n \n <li>FLZ-ZnO-PVA could be used as a suitable material for treatment of sepsis.</li>\n </ul>\n </div>\n </section>\n </div>","PeriodicalId":17662,"journal":{"name":"Journal of Vinyl & Additive Technology","volume":"30 4","pages":"969-982"},"PeriodicalIF":3.8000,"publicationDate":"2024-02-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Vinyl & Additive Technology","FirstCategoryId":"88","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/vnl.22098","RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, APPLIED","Score":null,"Total":0}

引用次数: 0

Abstract

Nanotechnology, a versatile field, holds promise in diverse applications, such as advanced pharmaceutical techniques and innovative chemical compound fabrication. Recently, the World Health Organization (WHO) has identified sepsis as a global health priority, attributing most sepsis-related deaths to the underlying infection. Sepsis is a complex disease that manifests in various ways, depending on factors, such as pathogen involved, mode of transmission, and the patient's immune competence. This study focuses on synthesizing zinc oxide (ZnO) through an in-situ precipitation method and employing a solution-based technique to coat the inorganic ZnO nanomaterial with the antimicrobial drug fluconazole (FLZ), resulting in a FLZ-ZnO composite. Further enhancement is achieved by modifying the composite with poly(vinyl alcohol) (PVA) to improve mechanical strength, physicochemical characteristics, and the interfacial network between ZnO and FLZ. Characterization through X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), field emission scanning electron microscopy (FESEM), particle size distribution (PSD), and thermo gravimetric analysis (TGA) confirms the synthesized compounds are stoichiometric in nature. The FLZ-ZnO-PVA nanocomposite demonstrates significant antifungal activity against C. albicans and A. niger, as well as enhanced antibacterial activity against E. coli and S. aureus evaluated through well diffusion technique. In vitro cellular compatibility assessment using the MTT assay with NIH-3T3 cells reveals exceptional viability (above 75%) and negligible cytotoxicity at a concentration of 1.56 μg/mL, indicating high biosafety. The FLZ-ZnO-PVA nanocomposite exhibits outstanding biological performance, making it a promising candidate for clinical applications in preventing sepsis and prospective infections.

Highlights

Developments and preparation of FLZ-ZnO-PVA nanocomposite.
FLZ-ZnO-PVA nanocomposite shows optimum antimicrobial activity.
FLZ-ZnO-PVA shows cytotoxicity against the mouse embryonic fibroblast cell line.
FLZ-ZnO-PVA could be used as a suitable material for treatment of sepsis.

Abstract Image

查看原文本刊更多论文

高效氟康唑负载氧化锌-聚乙烯醇纳米复合材料：生物应用的解释性断点

纳米技术是一个多才多艺的领域，在各种应用领域都大有可为，例如先进的制药技术和创新的化合物制造。最近，世界卫生组织（WHO）将败血症确定为全球健康的优先事项，并将大多数与败血症相关的死亡归因于潜在的感染。败血症是一种复杂的疾病，其表现形式多种多样，取决于所涉及的病原体、传播方式和患者的免疫能力等因素。本研究的重点是通过原位沉淀法合成氧化锌（ZnO），并采用基于溶液的技术在无机 ZnO 纳米材料上包覆抗菌药物氟康唑（FLZ），形成 FLZ-ZnO 复合材料。通过使用聚乙烯醇（PVA）对复合材料进行改性，提高了其机械强度、理化特性以及 ZnO 和 FLZ 之间的界面网络，从而进一步增强了其性能。通过 X 射线衍射 (XRD)、傅立叶变换红外光谱 (FTIR)、场发射扫描电子显微镜 (FESEM)、粒度分布 (PSD) 和热重分析 (TGA) 进行表征，证实了合成化合物的化学计量性质。通过井扩散技术评估，FLZ-ZnO-PVA 纳米复合材料对白僵菌和黑僵菌具有显著的抗真菌活性，对大肠杆菌和金黄色葡萄球菌具有更强的抗菌活性。使用 MTT 法对 NIH-3T3 细胞进行的体外细胞相容性评估显示，在 1.56 μg/mL 的浓度下，细胞存活率极高（超过 75%），细胞毒性可忽略不计，表明具有很高的生物安全性。FLZ-ZnO-PVA 纳米复合材料具有出色的生物学性能，因此有望在预防败血症和前瞻性感染方面得到临床应用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of Vinyl & Additive Technology 工程技术-材料科学：纺织

CiteScore

5.40

自引率

14.80%

发文量

审稿时长

>12 weeks

期刊介绍： Journal of Vinyl and Additive Technology is a peer-reviewed technical publication for new work in the fields of polymer modifiers and additives, vinyl polymers and selected review papers. Over half of all papers in JVAT are based on technology of additives and modifiers for all classes of polymers: thermoset polymers and both condensation and addition thermoplastics. Papers on vinyl technology include PVC additives.