Major crossmatch compatibility of rabbit blood with rabbit, canine, and feline blood

IF 1.1 3区农林科学 Q3 VETERINARY SCIENCES

Journal of veterinary emergency and critical care Pub Date : 2024-02-26 DOI:10.1111/vec.13362

Nicholas G. Dannemiller DVM, Sarah M. Ozawa DVM, DACZM, Olivia A. Petritz DVM, DACZM, Sarah E. Musulin DVM, DACVECC

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引用次数: 0

Abstract

Objective

To evaluate the major crossmatch compatibility between rabbit recipients, rabbit donors, and the major canine and feline blood types.

Design

Prospective in vitro study in December 2021.

Setting

Academic veterinary teaching hospital.

Animals

Whole blood samples were collected from 11 healthy New Zealand White rabbits (Oryctolagus cuniculus) with no previous transfusion history. Three pigtail segments were acquired from dog erythrocyte antigen (DEA)-1-positive, DEA-1-negative, and feline type A blood units. Whole blood was collected from a healthy type B blood donor cat.

Interventions

Blood from each rabbit recipient underwent a major crossmatch using standard tube crossmatch methodology with itself and the following donor blood types: rabbit, DEA-1-positive, DEA-1-negative, feline type A, and feline type B.

Measurements and Main Results

Self-crossmatches and crossmatches between rabbit recipients and conspecific donors were negative for hemolysis and agglutination. Crossmatches between rabbit recipients and canine and feline donors yielded no hemolysis but produced varying degrees of macroscopic and microscopic agglutination. Rabbit recipients had 1.4 (95% confidence interval: 1.1–1.8) times the risk of macroscopic agglutination when major crossmatched with canine blood compared to feline blood. No significant difference in agglutination was found between DEA-1-positive and DEA-1-negative or feline type A and type B donors.

Conclusions

These findings support allogeneic blood transfusions between rabbits being highly compatible and suggest rabbits have naturally occurring alloantibodies against both canine and feline red blood cells. However, feline red blood cells had a lower rate of in vitro incompatibility on major crossmatch, suggesting potentially higher in vivo compatibility if an emergency xenotransfusion is needed. Further prospective research is needed to determine if xenotransfusion is associated with a higher incidence of acute and delayed transfusion reactions in rabbits than allogeneic transfusions.

查看原文本刊更多论文

兔血与兔血、犬血和猫血的主要交叉配血相容性。

目的评估兔受体、兔供体与主要犬科和猫科动物血型之间的主要交叉配血相容性：设计：2021 年 12 月进行的前瞻性体外研究：学术兽医教学医院：从 11 只无输血史的健康新西兰白兔（Oryctolagus cuniculus）身上采集全血样本。从狗红细胞抗原（DEA）-1 阳性、DEA-1 阴性和猫 A 型血单位采集了三个尾段。从健康的 B 型供血猫身上采集全血：干预措施：采用标准试管交叉配血方法，对每只兔子受血者的血液与自身及以下供血者血型进行主要交叉配血：兔子、DEA-1 阳性、DEA-1 阴性、猫科动物 A 型和猫科动物 B 型：自交配血和兔受血者与同种供血者之间的交叉配血的溶血和凝集反应均为阴性。兔受体与犬科和猫科供体之间的交叉配血无溶血现象，但会产生不同程度的宏观和微观凝集。与猫科动物血液相比，兔子受体与犬科动物血液交叉配血时发生大体凝集的风险是猫科动物血液的 1.4 倍（95% 置信区间：1.1-1.8）。DEA-1阳性和DEA-1阴性或猫科动物A型和B型献血者之间的凝集没有明显差异：这些研究结果表明，兔子之间的同种异体输血具有高度相容性，并表明兔子对犬科和猫科动物的红细胞具有天然异体抗体。然而，猫科动物红细胞在体外主要交叉配血中的不相容性较低，这表明如果需要紧急异种输血，体内相容性可能更高。需要进一步开展前瞻性研究，以确定异种输血与同种异体输血相比，是否会导致兔子急性和延迟输血反应的发生率更高。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of veterinary emergency and critical care 农林科学-兽医学

CiteScore

2.50

自引率

15.40%

发文量

121

审稿时长

18-36 weeks

期刊介绍： The Journal of Veterinary Emergency and Critical Care’s primary aim is to advance the international clinical standard of care for emergency/critical care patients of all species. The journal’s content is relevant to specialist and non-specialist veterinarians practicing emergency/critical care medicine. The journal achieves it aims by publishing descriptions of unique presentation or management; retrospective and prospective evaluations of prognosis, novel diagnosis, or therapy; translational basic science studies with clinical relevance; in depth reviews of pertinent topics; topical news and letters; and regular themed issues. The journal is the official publication of the Veterinary Emergency and Critical Care Society, the American College of Veterinary Emergency and Critical Care, the European Veterinary Emergency and Critical Care Society, and the European College of Veterinary Emergency and Critical Care. It is a bimonthly publication with international impact and adheres to currently accepted ethical standards.