The inhibitory effect of apatinib on different small cell lung cancer cells and in lung cancer-bearing mice and patients

IF 1.9 4区医学 Q3 RESPIRATORY SYSTEM

Clinical Respiratory Journal Pub Date : 2024-02-25 DOI:10.1111/crj.13738

Mingtao Liu, Hui Li, Ranran Guo, Xia Yu, Yu Li

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引用次数: 0

Abstract

Purpose

To observe the inhibitory effect of the small molecule tyrosine kinase inhibitor apatinib on small cell lung cancer in vitro and vivo.

Material and methods

Three small lung cancer cells were selected CCK-8 and monoclonal assay was used to determine the effect of apatinib on proliferation. The effects on cell cycle and apoptosis were detected by flow cytometry and TUNEL. We observed the inhibitory effect of different doses of apatinib on xenograft tumor. The efficacy and safety of apatinib in 20 patients with advanced small cell lung cancer were observed.

Results

For small cell lung cancer with high expression of VEGFR2, apatinib has a significant inhibitory effect both in vitro and in vivo. It can play an inhibitory role by promoting apoptosis and cell cycle arrest pathways. For small cell lung cancer with low expression of VEGFR, the inhibitory effect on cells in vitro was not significant. It has certain inhibitory effect on nude mouse transplanted tumor and small cell lung cancer patients, but the effect is weaker than that of animal models and patients with small cell lung cancer cells with high expression of VEGFR2.

Conclusion

Apatinib has a significant inhibitory effect on small cell lung cancer with high expression of VEGFR2 and may be a treatment for small cell lung cancer patients.

Abstract Image

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阿帕替尼对不同小细胞肺癌细胞以及肺癌小鼠和患者的抑制作用。

目的：观察小分子酪氨酸激酶抑制剂阿帕替尼在体外和体内对小细胞肺癌的抑制作用：材料和方法：选取3个小细胞肺癌细胞，用CCK-8和单克隆检测法测定阿帕替尼对细胞增殖的影响。流式细胞术和 TUNEL 检测了阿帕替尼对细胞周期和凋亡的影响。我们观察了不同剂量的阿帕替尼对异种移植瘤的抑制作用。观察了阿帕替尼在20例晚期小细胞肺癌患者中的疗效和安全性：结果：对于VEGFR2高表达的小细胞肺癌，阿帕替尼在体外和体内均有显著的抑制作用。它可以通过促进细胞凋亡和细胞周期停滞途径发挥抑制作用。对于 VEGFR 低表达的小细胞肺癌，阿帕替尼在体外对细胞的抑制作用不明显。对裸鼠移植瘤和小细胞肺癌患者有一定的抑制作用，但效果弱于动物模型和VEGFR2高表达的小细胞肺癌细胞：阿帕替尼对血管内皮生长因子受体2高表达的小细胞肺癌有明显的抑制作用，可作为小细胞肺癌患者的治疗药物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Clinical Respiratory Journal 医学-呼吸系统

CiteScore

3.70

自引率

0.00%

发文量

104

审稿时长

>12 weeks

期刊介绍： Overview Effective with the 2016 volume, this journal will be published in an online-only format. Aims and Scope The Clinical Respiratory Journal (CRJ) provides a forum for clinical research in all areas of respiratory medicine from clinical lung disease to basic research relevant to the clinic. We publish original research, review articles, case studies, editorials and book reviews in all areas of clinical lung disease including: Asthma Allergy COPD Non-invasive ventilation Sleep related breathing disorders Interstitial lung diseases Lung cancer Clinical genetics Rhinitis Airway and lung infection Epidemiology Pediatrics CRJ provides a fast-track service for selected Phase II and Phase III trial studies. Keywords Clinical Respiratory Journal, respiratory, pulmonary, medicine, clinical, lung disease, Abstracting and Indexing Information Academic Search (EBSCO Publishing) Academic Search Alumni Edition (EBSCO Publishing) Embase (Elsevier) Health & Medical Collection (ProQuest) Health Research Premium Collection (ProQuest) HEED: Health Economic Evaluations Database (Wiley-Blackwell) Hospital Premium Collection (ProQuest) Journal Citation Reports/Science Edition (Clarivate Analytics) MEDLINE/PubMed (NLM) ProQuest Central (ProQuest) Science Citation Index Expanded (Clarivate Analytics) SCOPUS (Elsevier)