Identification and validation of LINC01322 as a potential prognostic biomarker and oncogene promoting tumor progression in lung adenocarcinoma

IF 3.4 3区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY
Lizhong Zeng , Ke Mi , Lei Peng , Haimei Wen , Ting Jiao , Xin Lyu , Tong Jiao , Yang Chen , Jingyan Yuan , Long Zhang , Shuanying Yang
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引用次数: 0

Abstract

Our study identified a novel long noncoding RNA, LINC01322, that acts as an oncogene in lung adenocarcinoma progression. Cytoplasmic and nuclear RNA purification assays indicated that LINC01322 was localized in the cytoplasm and nucleus. Gene set enrichment analysis revealed the involvement of LINC01322 in the regulation of cell proliferation, migration, and the Janus kinase/signal transducer and activator of transcription (JAK/STAT) signaling pathway. LINC01322 may promote lung adenocarcinoma proliferation and migration through the Janus kinase/signal transducer and activator of transcription signaling pathway. In vitro experiments demonstrated that the knockdown of LINC01322 significantly suppressed lung adenocarcinoma cell proliferation, migration, and activation of the Janus kinase 2/signal transducer and activator of transcription 3 signaling pathway, whereas overexpression had the opposite effects. Inhibition of the Janus kinase 2/signal transducer and activator of transcription 3 pathway activity partially reversed the enhancement of cell proliferation and migration caused by LINC01322 overexpression. In vivo experiments further verified the oncogene role of LINC01322. Altogether, our findings suggest that LINC01322 promotes lung adenocarcinoma progression by activating the Janus kinase 2/signal transducer and activator of transcription 3 signaling pathway and that it could be a therapeutic target.

鉴定和验证 LINC01322 是肺腺癌潜在的预后生物标记物和促进肿瘤进展的癌基因。
我们的研究发现了一种新型长非编码RNA--LINC01322,它是肺腺癌进展过程中的致癌基因。细胞质和细胞核 RNA 纯化试验表明,LINC01322 定位于细胞质和细胞核中。基因组富集分析显示,LINC01322参与了细胞增殖、迁移和Janus激酶/信号转导和激活转录(JAK/STAT)信号通路的调控。LINC01322可能通过Janus激酶/信号转导和激活转录信号通路促进肺腺癌的增殖和迁移。体外实验表明,敲除 LINC01322 能显著抑制肺腺癌细胞的增殖、迁移和 Janus 激酶 2/信号转导和转录激活因子 3 信号通路的激活,而过表达则有相反的效果。抑制Janus激酶2/信号转导子和转录激活子3通路的活性可部分逆转LINC01322过表达对细胞增殖和迁移的促进作用。体内实验进一步验证了LINC01322的癌基因作用。总之,我们的研究结果表明,LINC01322通过激活Janus激酶2/信号转导和激活剂转录3信号通路促进肺腺癌的进展,它可能是一个治疗靶点。
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来源期刊
CiteScore
8.10
自引率
0.00%
发文量
124
审稿时长
19 days
期刊介绍: IJBCB publishes original research articles, invited reviews and in-focus articles in all areas of cell and molecular biology and biomedical research. Topics of interest include, but are not limited to: -Mechanistic studies of cells, cell organelles, sub-cellular molecular pathways and metabolism -Novel insights into disease pathogenesis -Nanotechnology with implication to biological and medical processes -Genomics and bioinformatics
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