Development of cross-linked polyvinyl alcohol/sodium caseinate/silver nanoparticle electrospun mats for antibacterial wound dressing

IF 3.8 4区 工程技术 Q2 CHEMISTRY, APPLIED
Sema Samatya Yılmaz, Ayse Aytac
{"title":"Development of cross-linked polyvinyl alcohol/sodium caseinate/silver nanoparticle electrospun mats for antibacterial wound dressing","authors":"Sema Samatya Yılmaz,&nbsp;Ayse Aytac","doi":"10.1002/vnl.22094","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <p>In this study, nanofibers were produced by the electrospinning method by adding 1%, 3%, 5%, and 7% silver nanoparticles (Ag NPs) into the polyvinyl alcohol/sodium caseinate (60/40, w/w) mixture. Electrospun materials were cross-linked by immersion in a glutaraldehyde bath. Thus, antibacterial wound dressings that could be used on exuding wounds were developed. According to the scanning electron microscopy images of nanofibers, the cross-linking process by immersion method did not cause negative effects such as breakage or adhesion on the surface of fibers. The cross-linking of nanofibers was demonstrated by the presence of acetal linkages by Fourier-transform infrared spectroscopy analysis. The cross-linking process significantly improved the thermal properties of the nanofibers. The lowest crystallinity calculated in the differential scanning calorimeter was observed in the 3% Ag NP-added nanofiber with a value of 1.83%. It exhibited the lowest total soluble matter content with a value of 11.98% owing to its high cross-linking density. In addition, the 3% Ag NP-added nanofiber, which had the highest toughness and ductility, displayed the highest tensile stress with 3.5 MPa and the highest tensile strain with 7.53%. Moreover, it showed 78.11% cell viability on the L929 fibroblast cell line at the end of the 24th hour. It was indicated that although the electrospun mats maintained 100% antibacterial effectiveness, they could not be used as a wound dressing for 48 h. It was reported that only 3% Ag NP-added nanofiber could be used as a 100% effective antibacterial wound dressing for both <i>Escherichia coli</i> and <i>Staphylococcus aureus</i> provided that it was renewed every 24 h. Consequently, it was notified that cross-linking by the immersion method can be used in biomedical applications.</p>\n </section>\n \n <section>\n \n <h3> Highlights</h3>\n \n <div>\n <ul>\n \n <li>The antibacterial effects of Ag NP-doped PVA/NaCAS electrospun mats were significant.</li>\n \n <li>The cross-linking process did not induce toxic effects some of the nanofibers.</li>\n \n <li>High cross-linking density was observed in the nanofibers.</li>\n \n <li>The swelling property of nanofibers is a good feature required in an ideal wound dressing.</li>\n </ul>\n </div>\n </section>\n </div>","PeriodicalId":17662,"journal":{"name":"Journal of Vinyl & Additive Technology","volume":"30 4","pages":"911-928"},"PeriodicalIF":3.8000,"publicationDate":"2024-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Vinyl & Additive Technology","FirstCategoryId":"88","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/vnl.22094","RegionNum":4,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, APPLIED","Score":null,"Total":0}
引用次数: 0

Abstract

In this study, nanofibers were produced by the electrospinning method by adding 1%, 3%, 5%, and 7% silver nanoparticles (Ag NPs) into the polyvinyl alcohol/sodium caseinate (60/40, w/w) mixture. Electrospun materials were cross-linked by immersion in a glutaraldehyde bath. Thus, antibacterial wound dressings that could be used on exuding wounds were developed. According to the scanning electron microscopy images of nanofibers, the cross-linking process by immersion method did not cause negative effects such as breakage or adhesion on the surface of fibers. The cross-linking of nanofibers was demonstrated by the presence of acetal linkages by Fourier-transform infrared spectroscopy analysis. The cross-linking process significantly improved the thermal properties of the nanofibers. The lowest crystallinity calculated in the differential scanning calorimeter was observed in the 3% Ag NP-added nanofiber with a value of 1.83%. It exhibited the lowest total soluble matter content with a value of 11.98% owing to its high cross-linking density. In addition, the 3% Ag NP-added nanofiber, which had the highest toughness and ductility, displayed the highest tensile stress with 3.5 MPa and the highest tensile strain with 7.53%. Moreover, it showed 78.11% cell viability on the L929 fibroblast cell line at the end of the 24th hour. It was indicated that although the electrospun mats maintained 100% antibacterial effectiveness, they could not be used as a wound dressing for 48 h. It was reported that only 3% Ag NP-added nanofiber could be used as a 100% effective antibacterial wound dressing for both Escherichia coli and Staphylococcus aureus provided that it was renewed every 24 h. Consequently, it was notified that cross-linking by the immersion method can be used in biomedical applications.

Highlights

  • The antibacterial effects of Ag NP-doped PVA/NaCAS electrospun mats were significant.
  • The cross-linking process did not induce toxic effects some of the nanofibers.
  • High cross-linking density was observed in the nanofibers.
  • The swelling property of nanofibers is a good feature required in an ideal wound dressing.

Abstract Image

开发用于抗菌伤口敷料的交联聚乙烯醇/酪蛋白酸钠/纳米银电纺垫
本研究采用电纺丝法,在聚乙烯醇/酪蛋白酸钠(60/40,w/w)混合物中分别加入 1%、3%、5% 和 7% 的银纳米粒子(Ag NPs)制备纳米纤维。将电纺材料浸入戊二醛浴中进行交联。这样,可用于渗出伤口的抗菌伤口敷料就研制成功了。根据纳米纤维的扫描电子显微镜图像,浸泡法交联过程不会造成纤维断裂或表面粘连等负面影响。通过傅里叶变换红外光谱分析,纳米纤维的交联表现为乙缩醛连接的存在。交联过程大大改善了纳米纤维的热性能。在差示扫描量热计中计算出的结晶度最低的是添加了 3% Ag NP 的纳米纤维,为 1.83%。由于交联密度高,纳米纤维的总可溶物含量最低,为 11.98%。此外,添加了 3% Ag NP 的纳米纤维具有最高的韧性和延展性,其拉伸应力最大,为 3.5 兆帕,拉伸应变最大,为 7.53%。此外,在第 24 小时结束时,L929 成纤维细胞系的细胞存活率为 78.11%。据报道,只有添加了 3% Ag NP 的纳米纤维可用作对大肠杆菌和金黄色葡萄球菌100%有效的抗菌伤口敷料,条件是每24小时更新一次。亮点 掺 Ag NP 的 PVA/NaCAS 电纺垫抗菌效果显著。交联过程不会对部分纳米纤维产生毒性作用。纳米纤维的交联密度很高。纳米纤维的膨胀特性是理想伤口敷料所需的良好特性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Journal of Vinyl & Additive Technology
Journal of Vinyl & Additive Technology 工程技术-材料科学:纺织
CiteScore
5.40
自引率
14.80%
发文量
73
审稿时长
>12 weeks
期刊介绍: Journal of Vinyl and Additive Technology is a peer-reviewed technical publication for new work in the fields of polymer modifiers and additives, vinyl polymers and selected review papers. Over half of all papers in JVAT are based on technology of additives and modifiers for all classes of polymers: thermoset polymers and both condensation and addition thermoplastics. Papers on vinyl technology include PVC additives.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信