Time elapsed between ovulation and insemination determines the quality of fertilized rat oocytes

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2024-02-26 DOI:10.1262/jrd.2023-067

Naomi NAKAGATA, Satohiro NAKAO, Nobuyuki MIKODA, Katsuma YAMAGA, Toru TAKEO

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Abstract

Genetically modified rats are valuable models in human disease research. We recently developed an improved system for rat sperm cryopreservation and in vitro fertilization (IVF) that facilitates the efficient production and preservation of genetically modified rats. In the IVF procedure performed using frozen–thawed rat sperm, the IVF schedule is fixed to ensure timely hormone administration and oocyte collection. To enhance the flexibility of the IVF schedule, possible periods of postovulated rat oocytes with normal fertility and developmental abilities should be determined. Therefore, in this study, we examined the fertilization and developmental ability of incubated oocytes 1–13 h after oocyte collection at 9:00 AM. The fertilization rate decreased 7 h after oocyte collection, and abnormally fertilized oocytes appeared 10 h after oocyte collection. The developmental rate also decreased 7 h after oocyte collection; however, live pups were obtained from oocytes 12 h after oocyte collection. In summary, ovulated rat oocytes exhibited a high developmental ability after IVF for up to 4 h after oocyte collection.

Graphical Abstract Fullsize Image

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排卵和授精之间的时间间隔决定大鼠受精卵细胞的质量

转基因大鼠是人类疾病研究的重要模型。我们最近开发了一种改进的大鼠精子冷冻保存和体外受精（IVF）系统，有助于高效生产和保存转基因大鼠。在使用冷冻解冻的大鼠精子进行体外受精的过程中，体外受精的时间安排是固定的，以确保及时使用激素和采集卵母细胞。为了提高体外受精时间安排的灵活性，应确定具有正常生育能力和发育能力的大鼠卵母细胞可能的排卵期。因此，在本研究中，我们检测了上午 9:00 采集卵母细胞后 1-13 小时孵化卵母细胞的受精率和发育能力。受精率在卵母细胞采集 7 小时后下降，异常受精卵在卵母细胞采集 10 小时后出现。发育率在卵母细胞采集 7 小时后也有所下降；但在卵母细胞采集 12 小时后，仍能从卵母细胞中获得活幼鼠。总之，体外受精后的大鼠排卵卵母细胞在卵母细胞采集后 4 小时内表现出较高的发育能力。图文摘要全尺寸图片

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.