Aquaporin-5 Protein Is Selectively Reduced in Rat Parotid Glands under Conditions of Fasting or a Liquid Diet

IF 1.6 4区生物学 Q4 CELL BIOLOGY

Acta Histochemica Et Cytochemica Pub Date : 2024-02-23 DOI:10.1267/ahc.24-00003

Yoshie Fujita, Akie Taniguchi, Hanako Yamamoto, Hideru Obinata, Hiroshi Kogo, Akiko Iizuka-Kogo, Maiko Ikezawa, Yukiko Tajika, Satoshi Yokoo, Toshiyuki Matsuzaki

{"title":"Aquaporin-5 Protein Is Selectively Reduced in Rat Parotid Glands under Conditions of Fasting or a Liquid Diet","authors":"Yoshie Fujita, Akie Taniguchi, Hanako Yamamoto, Hideru Obinata, Hiroshi Kogo, Akiko Iizuka-Kogo, Maiko Ikezawa, Yukiko Tajika, Satoshi Yokoo, Toshiyuki Matsuzaki","doi":"10.1267/ahc.24-00003","DOIUrl":null,"url":null,"abstract":"Aquaporin-5 (AQP5) water channel, transmembrane protein 16A (TMEM16A) Ca2+-activated Cl− channel, and Na+-K+-2Cl− cotransporter (NKCC1) are membrane proteins on salivary gland acinar cells that function in watery saliva secretion. We examined their expression changes in rat parotid glands under reduced mastication. Rats were either fed regular chow as a control group, fasted for 48 hr or fed a liquid diet for 48 hr or 1 week to reduce mastication. The parotid glands were then resected to analyze the protein and mRNA levels by immunofluorescence, immunoblotting, and reverse-transcription quantitative PCR (RT-qPCR). AQP5 protein was significantly decreased in both liquid diet groups and the fasting group but its mRNA levels showed no apparent changes compared with the control group. The protein and mRNA levels of TMEM16A and NKCC1 showed no significant changes between any of the groups other than an increase in NKCC1 mRNA in the 1-week liquid diet group. These results suggest that reduced mastication may increase the AQP5 protein degradation, but not that of other membrane proteins necessary for saliva secretion.\n","PeriodicalId":6888,"journal":{"name":"Acta Histochemica Et Cytochemica","volume":"45 1","pages":""},"PeriodicalIF":1.6000,"publicationDate":"2024-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta Histochemica Et Cytochemica","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1267/ahc.24-00003","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"CELL BIOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

Aquaporin-5 (AQP5) water channel, transmembrane protein 16A (TMEM16A) Ca²⁺-activated Cl⁻ channel, and Na⁺-K⁺-2Cl⁻ cotransporter (NKCC1) are membrane proteins on salivary gland acinar cells that function in watery saliva secretion. We examined their expression changes in rat parotid glands under reduced mastication. Rats were either fed regular chow as a control group, fasted for 48 hr or fed a liquid diet for 48 hr or 1 week to reduce mastication. The parotid glands were then resected to analyze the protein and mRNA levels by immunofluorescence, immunoblotting, and reverse-transcription quantitative PCR (RT-qPCR). AQP5 protein was significantly decreased in both liquid diet groups and the fasting group but its mRNA levels showed no apparent changes compared with the control group. The protein and mRNA levels of TMEM16A and NKCC1 showed no significant changes between any of the groups other than an increase in NKCC1 mRNA in the 1-week liquid diet group. These results suggest that reduced mastication may increase the AQP5 protein degradation, but not that of other membrane proteins necessary for saliva secretion.

查看原文本刊更多论文

禁食或流质饮食条件下大鼠腮腺中的水蒸发蛋白-5 蛋白选择性减少

水蒸发素-5（AQP5）水通道、跨膜蛋白16A（TMEM16A）Ca2+激活的Cl-通道和Na+-K+-2Cl-共转运体（NKCC1）是唾液腺尖腺细胞上的膜蛋白，它们在唾液分泌中起着重要作用。我们研究了它们在咀嚼功能减弱的大鼠腮腺中的表达变化。将大鼠作为对照组，喂食普通饲料、禁食 48 小时或喂食流质食物 48 小时或 1 周以减少咀嚼。然后切除腮腺，通过免疫荧光、免疫印迹和反转录定量 PCR（RT-qPCR）分析蛋白质和 mRNA 水平。与对照组相比，流食组和禁食组的 AQP5 蛋白水平均明显下降，但其 mRNA 水平无明显变化。TMEM16A和NKCC1的蛋白和mRNA水平在各组间均无明显变化，只有一周流食组的NKCC1 mRNA水平有所上升。这些结果表明，咀嚼减少可能会增加 AQP5 蛋白的降解，但不会增加唾液分泌所需的其他膜蛋白的降解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Acta Histochemica Et Cytochemica 生物-细胞生物学

CiteScore

3.50

自引率

8.30%

发文量

审稿时长

>12 weeks

期刊介绍： Acta Histochemica et Cytochemica is the official online journal of the Japan Society of Histochemistry and Cytochemistry. It is intended primarily for rapid publication of concise, original articles in the fields of histochemistry and cytochemistry. Manuscripts oriented towards methodological subjects that contain significant technical advances in these fields are also welcome. Manuscripts in English are accepted from investigators in any country, whether or not they are members of the Japan Society of Histochemistry and Cytochemistry. Manuscripts should be original work that has not been previously published and is not being considered for publication elsewhere, with the exception of abstracts. Manuscripts with essentially the same content as a paper that has been published or accepted, or is under consideration for publication, will not be considered. All submitted papers will be peer-reviewed by at least two referees selected by an appropriate Associate Editor. Acceptance is based on scientific significance, originality, and clarity. When required, a revised manuscript should be submitted within 3 months, otherwise it will be considered to be a new submission. The Editor-in-Chief will make all final decisions regarding acceptance.