Vitamin D status alters genes involved in ovarian steroidogenesis in muskrat granulosa cells

IF 3.9 2区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Biochimica et biophysica acta. Molecular and cell biology of lipids Pub Date : 2024-02-23 DOI:10.1016/j.bbalip.2024.159469

Wenjing Lu, Yuan Chen, María Daniela Artigas Ramírez, Yuning Liu, Haolin Zhang, Zhengrong Yuan, Yingying Han, Qiang Weng

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引用次数: 0

Abstract

This study aims to explore the relationship between altered vitamin D (VitD₃) status and ovarian steroidogenesis in muskrats during the breeding and non-breeding seasons. During the breeding season, the ovaries of muskrats were observably enlarged and increased in weight, accompanied by elevated serum and ovarian VitD₃ status. Vitamin D receptor (VDR), VitD₃ metabolic molecules (CYP2R1, CYP27B1, and CYP24A1), and steroidogenic enzymes were immunolocalized in the ovarian cells of muskrats. The mRNA levels of VDR, CYP2R1, CYP27B1, and steroidogenic enzymes were considerably higher during the breeding season compared to the non-breeding season. RNA-seq analysis revealed a prominent enrichment of vitamin-related and ovarian steroidogenesis pathways. Furthermore, the addition of 1,25(OH)₂D₃ to the muskrat granulosa cells in vitro increased VDR and steroidogenic enzymes mRNA levels and enhanced the 17β-estradiol level. Overall, these findings supported that VitD₃ promotes the secretion of steroid hormones, thereby affecting seasonal changes in ovarian function in the muskrats.

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维生素 D 状态会改变麝鼠颗粒细胞中参与卵巢类固醇生成的基因

本研究旨在探讨麝鼠在繁殖期和非繁殖期维生素D（VitD3）状态的改变与卵巢类固醇生成之间的关系。在繁殖季节，麝鼠的卵巢明显增大，重量增加，同时血清和卵巢中的维生素 D3 状态升高。在麝鼠卵巢细胞中免疫定位了维生素 D 受体（VDR）、VitD3 代谢分子（CYP2R1、CYP27B1 和 CYP24A1）和类固醇生成酶。与非繁殖期相比，繁殖期麝鼠卵巢细胞中的VDR、CYP2R1、CYP27B1和类固醇生成酶的mRNA水平要高得多。RNA-seq分析表明，维生素相关途径和卵巢类固醇生成途径显著丰富。此外，体外向麝鼠颗粒细胞中添加1,25（OH）2D3可提高VDR和类固醇生成酶的mRNA水平，并提高17β-雌二醇水平。总之，这些研究结果表明，VitD3能促进类固醇激素的分泌，从而影响麝鼠卵巢功能的季节性变化。

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来源期刊

Biochimica et biophysica acta. Molecular and cell biology of lipids 生物-生化与分子生物学

CiteScore

11.00

自引率

2.10%

发文量

109

审稿时长

53 days

期刊介绍： BBA Molecular and Cell Biology of Lipids publishes papers on original research dealing with novel aspects of molecular genetics related to the lipidome, the biosynthesis of lipids, the role of lipids in cells and whole organisms, the regulation of lipid metabolism and function, and lipidomics in all organisms. Manuscripts should significantly advance the understanding of the molecular mechanisms underlying biological processes in which lipids are involved. Papers detailing novel methodology must report significant biochemical, molecular, or functional insight in the area of lipids.