Marilyn Strong-Townsend , Niora Fabian , Gerry Skinner , Rachel Murphy , Evan Hegarty , Sarah Peterson , Michael Coyne
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引用次数: 0
Abstract
Background
Chronic kidney disease (CKD) is common in older rabbits. The renal biomarkers blood urea and creatinine can be insensitive as indicators of renal disease in rabbits. Symmetric dimethylarginine (SDMA) has been shown to be an earlier and more sensitive biomarker for the assessment of glomerular filtration rate and the evaluation of CKD in several animal species. An immunoassay for SDMA has been validated in a number of species. The purpose of this study was to validate the SDMA immunoassay for rabbit serum and plasma and to determine the normal rabbit reference interval (RI) for SDMA.
Methods
Clinically healthy rabbits (n = 167) between 5 months and 8 years of age, of any breed or sex, participated in the study. Heparinized plasma or serum was collected for clinical chemistry and used for assay validation. Analytical validation of the SDMA immunoassay for rabbit serum and plasma was performed and included assessment of assay accuracy, precision, analytical sensitivity, linearity, interference testing, and plasma/serum bias. The SDMA reference interval (RI) was determined.
Results
The SDMA immunoassay met all set quality requirements assessed in analytical validation. The RI for SDMA was determined to be between 4–18 µg/dL.
Conclusions and clinical relevance
The SDMA immunoassay demonstrated acceptable performance in both rabbit serum and heparinized plasma as determined by the validation criteria. The utility of SDMA as a biomarker of renal disease in rabbits has not been determined yet.
期刊介绍：
The Journal of Exotic Pet Medicine provides clinicians with a convenient, comprehensive, "must have" resource to enhance and elevate their expertise with exotic pet medicine. Each issue contains wide ranging peer-reviewed articles that cover many of the current and novel topics important to clinicians caring for exotic pets. Diagnostic challenges, consensus articles and selected review articles are also included to help keep veterinarians up to date on issues affecting their practice. In addition, the Journal of Exotic Pet Medicine serves as the official publication of both the Association of Exotic Mammal Veterinarians (AEMV) and the European Association of Avian Veterinarians (EAAV). The Journal of Exotic Pet Medicine is the most complete resource for practitioners who treat exotic pets.
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