Mechanism underlying the rapid growth of Phalaenopsis equestris induced by 60Co-γ-ray irradiation.

IF 2.3 3区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Yang Meng, Wei Li, Yunxiao Guan, Zihan Song, Guoren He, Donghui Peng, Feng Ming
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引用次数: 0

Abstract

Gamma (γ)-ray irradiation is one of the important modern breeding methods. Gamma-ray irradiation can affect the growth rate and other characteristics of plants. Plant growth rate is crucial for plants. In horticultural crops, the growth rate of plants is closely related to the growth of leaves and flowering time, both of which have important ornamental value. In this study, 60Co-γ-ray was used to treat P. equestris plants. After irradiation, the plant's leaf growth rate increased, and sugar content and antioxidant enzyme activity increased. Therefore, we used RNA-seq technology to analyze the differential gene expression and pathways of control leaves and irradiated leaves. Through transcriptome analysis, we investigated the reasons for the rapid growth of P. equestris leaves after irradiation. In the analysis, genes related to cell wall relaxation and glucose metabolism showed differential expression. In addition, the expression level of genes encoding ROS scavenging enzyme synthesis regulatory genes increased after irradiation. We identified two genes related to P. equestris leaf growth using VIGS technology: PeNGA and PeEXPA10. The expression of PeEXPA10, a gene related to cell wall expansion, was down-regulated, cell wall expansion ability decreased, cell size decreased, and leaf growth rate slowed down. The TCP-NGATHA (NGA) molecular regulatory module plays a crucial role in cell proliferation. When the expression of the PeNGA gene decreases, the leaf growth rate increases, and the number of cells increases. After irradiation, PeNGA and PeEXPA10 affect the growth of P. equestris leaves by influencing cell proliferation and cell expansion, respectively. In addition, many genes in the plant hormone signaling pathway show differential expression after irradiation, indicating the crucial role of plant hormones in plant leaf growth. This provides a theoretical basis for future research on leaf development and biological breeding.

Abstract Image

60Co-γ 射线辐照诱导蝴蝶兰快速生长的机制
伽马(γ)射线辐照是重要的现代育种方法之一。伽马射线辐照会影响植物的生长速度和其他特性。植物的生长速度对植物至关重要。在园艺作物中,植物的生长速度与叶片的生长和开花时间密切相关,两者都具有重要的观赏价值。本研究使用 60Co-γ 射线处理马齿苋植株。辐照后,植物的叶片生长速度加快,糖含量和抗氧化酶活性提高。因此,我们利用 RNA-seq 技术分析了对照叶片和辐照叶片的不同基因表达和通路。通过转录组分析,我们研究了辐照后马尾松叶片快速生长的原因。在分析中,与细胞壁松弛和葡萄糖代谢相关的基因出现了差异表达。此外,编码 ROS 清除酶合成调控基因的表达水平在辐照后有所增加。我们利用 VIGS 技术发现了两个与马齿苋叶片生长相关的基因:PeNGA 和 PeEXPA10。与细胞壁扩张相关的基因 PeEXPA10 表达下调,细胞壁扩张能力下降,细胞体积缩小,叶片生长速度减慢。TCP-NGATHA(NGA)分子调控模块在细胞增殖中起着至关重要的作用。当 PeNGA 基因表达量减少时,叶片生长速度加快,细胞数量增加。辐照后,PeNGA 和 PeEXPA10 分别通过影响细胞增殖和细胞扩增来影响马尾藻叶片的生长。此外,植物激素信号通路中的许多基因在辐照后出现差异表达,表明植物激素在植物叶片生长中起着至关重要的作用。这为今后的叶片发育和生物育种研究提供了理论依据。
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来源期刊
Molecular Genetics and Genomics
Molecular Genetics and Genomics 生物-生化与分子生物学
CiteScore
5.10
自引率
3.20%
发文量
134
审稿时长
1 months
期刊介绍: Molecular Genetics and Genomics (MGG) publishes peer-reviewed articles covering all areas of genetics and genomics. Any approach to the study of genes and genomes is considered, be it experimental, theoretical or synthetic. MGG publishes research on all organisms that is of broad interest to those working in the fields of genetics, genomics, biology, medicine and biotechnology. The journal investigates a broad range of topics, including these from recent issues: mechanisms for extending longevity in a variety of organisms; screening of yeast metal homeostasis genes involved in mitochondrial functions; molecular mapping of cultivar-specific avirulence genes in the rice blast fungus and more.
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